Transient Kinetic Analysis of SWR1C-Catalyzed H2A.Z Deposition Unravels the Impact of Nucleosome Dynamics and the Asymmetry of Histone Exchange

Autor: Raushan K. Singh, Jiayl Fan, Nathan Gioacchini, Shinya Watanabe, Osman Bilsel, Craig L. Peterson

Publikováno v: Cell Reports, Vol 27, Iss 2, Pp 374-386.e4 (2019)

Summary: The SWR1C chromatin remodeling enzyme catalyzes ATP-dependent replacement of nucleosomal H2A with the H2A.Z variant, regulating key DNA-mediated processes such as transcription and DNA repair. Here, we investigate the transient kinetic mecha

Externí odkaz: https://doaj.org/article/697c802901aa4c309a644156a7071cae

Metal-free ALS variants of dimeric human Cu,Zn-superoxide dismutase have enhanced populations of monomeric species.

Autor: Anna-Karin E Svensson, Osman Bilsel, Can Kayatekin, Jessica A Adefusika, Jill A Zitzewitz, C Robert Matthews

Publikováno v: PLoS ONE, Vol 5, Iss 4, p e10064 (2010)

Amino acid replacements at dozens of positions in the dimeric protein human, Cu,Zn superoxide dismutase (SOD1) can cause amyotrophic lateral sclerosis (ALS). Although it has long been hypothesized that these mutations might enhance the populations of

Externí odkaz: https://doaj.org/article/cc9477ab90c740ac907c19e1b7202efd

Creating round focused micro-jets from rectangular nozzles

Autor: Venkatesh Inguva, Blair Perot, Joachim Schulz, Rita Graceffa, Osman Bilsel

Publikováno v: Journal of Mechanical Science and Technology. 33:4281-4289

A focused jet is an axisymmetric jet of liquid surrounded by an outer coaxial gas jet. The gas jet is typically used to compress the liquid jet in the radial direction thereby focusing it. At microscales, it is difficult to manufacture micro-scale de

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b5a7694df755a4c12d7ded66c0571d6d
https://doi.org/10.1007/s12206-019-0824-x

ALS-linked PFN1 variants exhibit loss and gain of functions in the context of formin-induced actin polymerization

Autor: Daryl A. Bosco, Francesca Massi, Brittany R. Morgan, Eric J. Schmidt, Salome Funes, Antoine Jégou, Sivakumar Boopathy, Osman Bilsel, Lauren C. O’Connor, Jeanne E. McKeon

Publikováno v: Proc Natl Acad Sci U S A
Proceedings of the National Academy of Sciences
Proceedings of the National Academy of Sciences of the United States of America
Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2021, 118 (23), pp.e2024605118. ⟨10.1073/pnas.2024605118⟩

International audience; Profilin-1 (PFN1) plays important roles in modulating actin dynamics through binding both monomeric actin and proteins enriched with polyproline motifs. Mutations in PFN1 have been linked to the neurodegenerative disease amyot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::439ea8326d2e7709ba14eb274303e794
https://doi.org/10.1073/pnas.2024605118

In Situ Measurements of Polymer Micellization Kinetics with Millisecond Temporal Resolution

Autor: Ying Liu, Weifeng Shang, Pin Zhang, Paola Leon-Plata, Joseph Kalkowski, Thomas C. Irving, Chang Liu, Osman Bilsel, Magdalena Szymusiak

Publikováno v: Macromolecules

Utilizing synchrotron small-angle X-ray scattering (SAXS) integrated with a microfluidic device, micellization kinetics of a diblock co-polymer, poly(ethylene glycol)-b-poly(caprolactone), was measured in situ with millisecond temporal and micrometer

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::371a264f7b9ce514877581801d68b1d0
https://pubmed.ncbi.nlm.nih.gov/31588150