Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Oscar‐Werner Reif"'
Publikováno v:
Engineering in Life Sciences. 22:334-343
The interest in extracellular vesicles (EVs) has been increased in recent years due to their potential application in diagnosis and therapy of severe diseases. The versatile fields of application due to the numerous possible cargos and the targeted d
Autor:
Thomas Scheper, Daniela Harkensee, Robert Zeidler, Öznur Kökpinar, Cornelia Kasper, Roland Ulber, Oscar-Werner Reif
Publikováno v:
Biotechnology Progress. 22:1215-1219
To develop the most efficient strategy for the purification of proteins, two types of adsorber membrane devices with different functionalities were designed and tested: 8-strips and single spin columns. The most suitable type of membrane adsorber and
Publikováno v:
Journal of Chromatography A. 728:129-137
Membrane adsorbers (MAs) are used for protein separation in controlled mixed-mode interaction chromatography. The strong anion- and cation-exchange MAs used are made either from a synthetic copolymer or from modified cellulose membranes. The affinity
Publikováno v:
Cytotechnology. 21:205-215
The importance of mammalian cell cultures for biotechnological production processes is steadily increasing, despite the high demands of these organisms on their culture conditions. Efforts towards a more efficient bioprocess generally concentrate on
Autor:
Ruth Freitag, Oscar-Werner Reif
Publikováno v:
Journal of Chromatography A. 716:363-369
Capillary zone electrophoresis (CZE) with laser-induced fluorescence (LIF) detection is shown to constitute a unique technique for the investigation of the interaction between proteases, protease inhibitors and substrates. Under optimized analysis co
Publikováno v:
Analytical Chemistry. 66:4027-4033
Antibodies from human sera (h-IgG) were tagged with a fluorescent dye (fluorescein isothiocyanate, FITC) through the affinity reaction of FITC-labeled protein G with the Fc fragment of the antibodies. The complexes were quantified by capillary zone e
Autor:
Ruth Freitag, Oscar-Werner Reif, Uwe T. Bornscheuer, Ralf Dr. Lausch, Uldrich Menge, Thomas Scheper, Fragiskos N. Kolisis
Publikováno v:
Biochimica et Biophysica Acta (BBA) - General Subjects. 1201:55-60
Commercial lipase (triacylglycerol lipase, EC 3.1.1.3) of Pseudomonas cepacia (Amano) has been purified to homogeneity by a single chromatography on phenyl Sepharose. The eluted lipase crystallized spontaneously at 4 degrees C in the eluent, containi
Publikováno v:
Journal of Chromatography A. 664:13-25
Novel immobilized metal affinity membrane adsorbers (IMA-MA) were studied for potential use as stationary phases for protein separation. Protein adsorption on IMA-MA loaded with Cu(II), Ni(II), ZN(II) and Co(II) ions was compared as a function of the
Autor:
Thorsten Adams, Edward Balbirnie, Wolfgang Kahlert, Francis Verhoeye, Oscar-Werner Reif, Gerhard Greller, Davy de Wilde, Ute Noack
Publikováno v:
Single-Use Technology in Biopharmaceutical Manufacture
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::449d96989e378821d5fd75e12abc4f50
https://doi.org/10.1002/9780470909997.ch19
https://doi.org/10.1002/9780470909997.ch19
Autor:
Alexander Tappe, Cornelia Kasper, Thomas Scheper, Oscar-Werner Reif, Johanna-Gabriela Walter, Robert Zeidler
Publikováno v:
Cell Technology for Cell Products ISBN: 9781402054754
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5b16902fc5e7ee8bb430c338d4b56659
https://doi.org/10.1007/978-1-4020-5476-1_115
https://doi.org/10.1007/978-1-4020-5476-1_115