Zobrazeno 1 - 10
of 574
pro vyhledávání: '"Osamu, Nureki"'
Autor:
Takaaki A. Kobayashi, Hiroto Shimada, Fumiya K. Sano, Yuzuru Itoh, Sawako Enoki, Yasushi Okada, Tsukasa Kusakizako, Osamu Nureki
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-12 (2024)
Abstract Vitamin C plays important roles as a cofactor in many enzymatic reactions and as an antioxidant against oxidative stress. As some mammals including humans cannot synthesize vitamin C de novo from glucose, its uptake from dietary sources is e
Externí odkaz:
https://doaj.org/article/f5a87d612bbc4dee96b6a0b432ab3cc5
Autor:
Toshihiro Nakane, Ryoya Nakagawa, Soh Ishiguro, Sae Okazaki, Hideto Mori, Yutaro Shuto, Keitaro Yamashita, Nozomu Yachie, Hiroshi Nishimasu, Osamu Nureki
Publikováno v:
Communications Biology, Vol 7, Iss 1, Pp 1-10 (2024)
Abstract The RNA-guided DNA endonuclease Cas9 cleaves double-stranded DNA targets complementary to an RNA guide, and is widely used as a powerful genome-editing tool. Here, we report the crystal structure of Brevibacillus laterosporus Cas9 (BlCas9, a
Externí odkaz:
https://doaj.org/article/783bfe326ea64e06babe63f8818ac743
Autor:
Sundaram Acharya, Asgar Hussain Ansari, Prosad Kumar Das, Seiichi Hirano, Meghali Aich, Riya Rauthan, Sudipta Mahato, Savitri Maddileti, Sajal Sarkar, Manoj Kumar, Rhythm Phutela, Sneha Gulati, Abdul Rahman, Arushi Goel, C. Afzal, Deepanjan Paul, Trupti Agrawal, Vinay Kumar Pulimamidi, Subhadra Jalali, Hiroshi Nishimasu, Indumathi Mariappan, Osamu Nureki, Souvik Maiti, Debojyoti Chakraborty
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-23 (2024)
Abstract The clinical success of CRISPR therapies hinges on the safety and efficacy of Cas proteins. The Cas9 from Francisella novicida (FnCas9) is highly precise, with a negligible affinity for mismatched substrates, but its low cellular targeting e
Externí odkaz:
https://doaj.org/article/967b3513bca24917a4d3b9306cf6ba0f
Autor:
Aika Iwama, Ryoji Kise, Hiroaki Akasaka, Fumiya K. Sano, Hidetaka S. Oshima, Asuka Inoue, Wataru Shihoya, Osamu Nureki
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)
Abstract Pyroglutamylated RF-amide peptide (QRFP) is a peptide hormone with a C-terminal RF-amide motif. QRFP selectively activates a class A G-protein-coupled receptor (GPCR) GPR103 to exert various physiological functions such as energy metabolism
Externí odkaz:
https://doaj.org/article/b6422e77482146409dd13929fed4321c
Autor:
Kazuhiro Ishibashi, Satoru Sukegawa, Masaki Endo, Naho Hara, Osamu Nureki, Hiroaki Saika, Seiichi Toki
Publikováno v:
Frontiers in Plant Science, Vol 15 (2024)
Because virus vectors can spread systemically autonomously, they are powerful vehicles with which to deliver genome-editing tools into plant cells. Indeed, a vector based on a positive-strand RNA virus, potato virus X (PVX), harboring SpCas9 and its
Externí odkaz:
https://doaj.org/article/294dd67f98a94dbeaf99f1b92fd07bf5
Autor:
Shuhei Onishi, Kotone Uchiyama, Ko Sato, Chikako Okada, Shunsuke Kobayashi, Keisuke Hamada, Tomohiro Nishizawa, Osamu Nureki, Kazuhiro Ogata, Toru Sengoku
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-11 (2024)
Abstract Histone H2B monoubiquitination (at Lys120 in humans) regulates transcription elongation and DNA repair. In humans, H2B monoubiquitination is catalyzed by the heterodimeric Bre1 complex composed of Bre1A/RNF20 and Bre1B/RNF40. The Bre1 protei
Externí odkaz:
https://doaj.org/article/da2110e1d1e94ea08bb735896aa0317c
Autor:
Satoshi Watanabe, Yoshiaki Kise, Kento Yonezawa, Mariko Inoue, Nobutaka Shimizu, Osamu Nureki, Kenji Inaba
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-13 (2024)
Abstract ERGIC-53 transports certain subsets of newly synthesized secretory proteins and membrane proteins from the endoplasmic reticulum to the Golgi apparatus. Despite numerous structural and functional studies since its identification, the overall
Externí odkaz:
https://doaj.org/article/07a06575a85540998ba2c1a4f3a18c44
Autor:
Airi Jo-Watanabe, Toshiki Inaba, Takahiro Osada, Ryota Hashimoto, Tomohiro Nishizawa, Toshiaki Okuno, Sayoko Ihara, Kazushige Touhara, Nobutaka Hattori, Masatsugu Oh-Hora, Osamu Nureki, Takehiko Yokomizo
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024)
Abstract Homoeostatic regulation of the acid–base balance is essential for cellular functional integrity. However, little is known about the molecular mechanism through which the acid–base balance regulates cellular responses. Here, we report tha
Externí odkaz:
https://doaj.org/article/f95ce9a7d6854817a73fa616defc04d5
Autor:
Tamaki Izume, Ryo Kawahara, Akiharu Uwamizu, Luying Chen, Shun Yaginuma, Jumpei Omi, Hiroki Kawana, Fengjue Hou, Fumiya K. Sano, Tatsuki Tanaka, Kazuhiro Kobayashi, Hiroyuki H. Okamoto, Yoshiaki Kise, Tomohiko Ohwada, Junken Aoki, Wataru Shihoya, Osamu Nureki
Publikováno v:
Nature Communications, Vol 15, Iss 1, Pp 1-15 (2024)
Abstract GPR34 is a recently identified G-protein coupled receptor, which has an immunomodulatory role and recognizes lysophosphatidylserine (LysoPS) as a putative ligand. Here, we report cryo-electron microscopy structures of human GPR34-Gi complex
Externí odkaz:
https://doaj.org/article/bceac4aee4d24bd1b9b9d0c676409f20
PAM-flexible Cas9-mediated base editing of a hemophilia B mutation in induced pluripotent stem cells
Autor:
Takafumi Hiramoto, Yuji Kashiwakura, Morisada Hayakawa, Nemekhbayar Baatartsogt, Nobuhiko Kamoshita, Tomoyuki Abe, Hiroshi Inaba, Hiroshi Nishimasu, Hideki Uosaki, Yutaka Hanazono, Osamu Nureki, Tsukasa Ohmori
Publikováno v:
Communications Medicine, Vol 3, Iss 1, Pp 1-12 (2023)
Abstract Background Base editing via CRISPR-Cas9 has garnered attention as a method for correcting disease-specific mutations without causing double-strand breaks, thereby avoiding large deletions and translocations in the host chromosome. However, i
Externí odkaz:
https://doaj.org/article/8d87658f0b3149538b80ca60fb8ca67c