Bacteriophage genome engineering with CRISPR-Cas13a

Autor: Jingwen Guan, Agnès Oromí-Bosch, Senén D. Mendoza, Shweta Karambelkar, Joel D. Berry, Joseph Bondy-Denomy

Publikováno v: Nature microbiology, vol 7, iss 12
Nat Microbiol

Jumbo phages such as Pseudomonas aeruginosa ФKZ have potential as antimicrobials and as a model for uncovering basic phage biology. Both pursuits are currently limited by a lack of genetic engineering tools due to a proteinaceous 'phage nucleus' str

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2d537746b10032539efc3406c634a479
https://escholarship.org/uc/item/2js5z6p9

For analyzing the subcellular location of phiKZ phage nucleus

Autor: Guan, Jingwen, Oromí-Bosch, Agnès, Mendoza, Senén, Karambelkar, Shweta, Berry, Joel, Bondy-Denomy, Joseph

The python code was used to generate the distribution of the subcellular location of the phage nucleus for WT phiKZ, mutant∆phuZ, and∆phuZ complemented in trans.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0ecf4170993f813ed38702c6f47bcf7d

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Rapid assessment of changes in phage bioactivity using dynamic light scattering.

Autor: Dharmaraj T; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.; Sarafan ChEM-H, Stanford University, Stanford, CA 94305, USA., Kratochvil MJ; Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305., Pourtois JD; Hopkins Marine Station, Department of Biology, Stanford University, Pacific Grove, CA 93950, USA., Chen Q; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Hajfathalian M; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Hargil A; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Lin YH; Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA., Evans Z; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Oromí-Bosch A; Felix Biotechnology, South San Francisco, CA, 94080., Berry JD; Felix Biotechnology, South San Francisco, CA, 94080., McBride R; Felix Biotechnology, South San Francisco, CA, 94080., Haddock NL; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Holman DR; Division of Gastroenterology and Hepatology, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., van Belleghem JD; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Chang TH; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA., Barr JJ; School of Biological Sciences, Monash University, Clayton, 3800, VIC, Australia., Lavigne R; Department of Biosystems, KU Leuven, Leuven, 3001, Belgium., Heilshorn SC; Department of Materials Science and Engineering, Stanford University, Stanford, CA 94305., Blankenberg FG; Division of Pediatric Radiology and Nuclear Medicine, Department of Radiology, Lucile Packard Children's Hospital, Stanford, CA 94305, USA., Bollyky PL; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2023 Jul 02. Date of Electronic Publication: 2023 Jul 02.