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A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer.

Autor: Brad Ebanks, Pola Kwiecinska, Nicoleta Moisoi, Lisa Chakrabarti

Publikováno v: PLoS ONE, Vol 18, Iss 7, p e0276147 (2023)

High-resolution respirometry methods allow for the assessment of oxygen consumption by the electron transfer systems within cells, tissue samples, and isolated mitochondrial preparations. As mitochondrial integrity is compromised by the process of cr

Externí odkaz: https://doaj.org/article/78672b1ab0a34f7e98856145752a5de2

Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer

Autor: Justin J. Crowder, Ziqian Zeng, Alissa N. Novak, Nathan J. Alves, Amelia K. Linnemann

Publikováno v: Islets. 14:128-138

Metabolic dysfunction of β-cells has been implicated as a contributor to diabetes pathogenesis, and efforts are ongoing to optimize analytical techniques that evaluate islet metabolism. High-resolution respirometry offers sensitive measurements of t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9702ef2a2b3eb3907c3ee08415abb2a9
https://doi.org/10.1080/19382014.2022.2054251

ETNK1 ChIP-Seq and Oroboros Respiratory Data

Autor: Piazza, Rocco

We generated ChIP-Seq data for the CRISPR ETNK1-N244S line using an antibody raised against the oxoG and we compared the oxoG signal with the WT one. The analysis of the oxoG signal at whole-genome level using custom bioinformatics tools revealed a s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::6e64cfa178598d128f51f7689e77d5b5

Assessing Mitochondrial Bioenergetics in Isolated Mitochondria from Mouse Heart Tissues Using Oroboros 2k-Oxygraph.

Autor: Long Q; Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. qqlong@tjh.tjmu.edu.cn., Huang L; School of Basic Medicine, Research Center of Integrative Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China., Huang K; Department of Cardiovascular Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China., Yang Q; Division of Cardiology, Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China. qyang@uab.edu.; Department of Nutrition Sciences, University of Alabama at Birmingham, Birmingham, AL, USA. qyang@uab.edu.

Publikováno v: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2019; Vol. 1966, pp. 237-246.