Assigning quantitative function to post-translational modifications reveals multiple sites of phosphorylation that tune yeast pheromone signaling output.

Autor: David Pincus, Christopher J Ryan, Richard D Smith, Roger Brent, Orna Resnekov

Publikováno v: PLoS ONE, Vol 8, Iss 3, p e56544 (2013)

Cell signaling systems transmit information by post-translationally modifying signaling proteins, often via phosphorylation. While thousands of sites of phosphorylation have been identified in proteomic studies, the vast majority of sites have no kno

Externí odkaz: https://doaj.org/article/71955dffe7204db7ae5a894e6264cdd0

Engineering allosteric regulation in protein kinases

Autor: David Pincus, Kimberly A. Reynolds, Jai P. Pandey, Orna Resnekov, Pau Creixell, Zoë A. Feder

Publikováno v: Science signaling. 11(555)

Phosphoregulation, in which the addition of a negatively charged phosphate group modulates protein activity, enables dynamic cellular responses. To understand how new phosphoregulation might be acquired, we mutationally scanned the surface of a proto

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::34943d2ff0896f66dfb20f5e3bf6e82f
https://pubmed.ncbi.nlm.nih.gov/30401787

Single-cell measurements of enzyme levels as a predictive tool for cellular fates during organic acid production

Autor: C. Gustavo Pesce, Laura Ruohonen, Merja Penttilä, Stefan Zdraljevic, Jussi Jäntti, Drew T. Wagner, Kevin Cheng, Orna Resnekov

Publikováno v: Zdraljevic, S, Wagner, D, Cheng, K, Ruohonen, L, Jäntti, J, Penttilä, M, Resnekov, O & Pesce, C G 2013, ' Single-cell measurements of enzyme levels as a predictive tool for cellular fates during organic acid production ', Applied and Environmental Microbiology, vol. 79, no. 24, pp. 7569-7582 . https://doi.org/10.1128/AEM.01749-13

Organic acids derived from engineered microbes can replace fossil-derived chemicals in many applications. Fungal hosts are preferred for organic acid production because they tolerate lignocellulosic hydrolysates and low pH, allowing economic producti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb9824ad8bb9abb0a2800615270f3a84
https://cris.vtt.fi/en/publications/52a9f54d-23b9-40a0-840c-2af0b4b058e7

Reagents for investigating MAPK signalling in model yeast species

Autor: David Pincus, Orna Resnekov, Annie Ening Tsong, Kirsten R. Benjamin, Ian E. Burbulis

Publikováno v: Yeast. 27:423-430

Here we present a set of resources (bacterial expression plasmids and antibodies) for the interrogation of proteins involved in yeast MAPK signalling. We constructed bacterial protein expression plasmids for 25 proteins involved in MAPK signalling in

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::851bf07a1f6dac9ec6040c4d575e7686
https://doi.org/10.1002/yea.1758

Post-transcriptional Control of Gene Expression

Autor: Orna Resnekov, Alexander von Gabain

Many important cellular processes rely on posttranscriptional control of gene expression. This book describes the mechanisms of gene expression at this level that occur in the cytoplasm of prokaryotes and eukaryotes. Several introductory chapters dis