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pro vyhledávání: '"Orli Lipshitz"'
Autor:
Nava Chapnik-Cohen, Orli Lipshitz, Avigdor Levanon, Mira Fine, Edna Sakal, Violet Daniel, Arieh Gertler, Dorit Vashdi
Publikováno v:
Fish Physiology and Biochemistry. 11:353-361
Carp growth hormone (cGH) cDNA, in which Cys-123 was mutated to Ala, was prepared, transferred to the expression vector, expressed in Escherichia coli and the mutant was purified to homogeneity. The mutation only slightly improved yield of the monome
Autor:
Edna Sakal, Avigdor Levanon, Violet Daniel, Orli Lipshitz, Arieh Gertler, Dorit Vashdi, Mira Fine
Publikováno v:
General and Comparative Endocrinology. 89:51-61
Carp growth hormone (cGH) cDNA (Koren et al., 1989) was cloned under the control of lambda-phage PLOL promoter and lambda cll ribosomal binding site into pBR322 plasmid to enable its expression in Escherichia coli A1645 that produces constitutively t