Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Organoids/cytology"'
Autor:
Naomi Moris, Julia Schröder, Sabitri Ghimire, Alexander van Oudenaarden, Susanne C. van den Brink, Alfonso Martinez Arias, Tina Balayo, Kerim Anlas, Anna Alemany
Publikováno v:
Nature, 582(7812), 410-415. Nature Publishing Group
Nature
Nature
The body plan of the mammalian embryo is shaped through the process of gastrulation, an early developmental event that transforms an isotropic group of cells into an ensemble of tissues that is ordered with reference to three orthogonal axes1. Althou
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47e426d3042fc0f6f8722ca81b317059
https://doi.org/10.1038/s41586-020-2383-9
https://doi.org/10.1038/s41586-020-2383-9
Autor:
Arne Van Hoeck, Jens Puschhof, Axel Rosendahl Huber, Ruben van Boxtel, Reinier van der Linden, Fernanda L. Paganelli, K. Christopher Garcia, Stefan van der Elst, Matthew Meyerson, Hans Clevers, Guillaume Dalmasso, Marios Giannakis, Richard Bonnet, Paul B. Stege, Phil Quirke, Tomohiro Mizutani, Henry M. Wood, Edwin Cuppen, Rob J. L. Willems, Maarten H. Geurts, Carino Gurjao, Cayetano Pleguezuelos-Manzano, Joep Beumer, Freek Manders, Yi Miao, Janetta Top, Jason Nomburg
Publikováno v:
Nature
Nature, Nature Publishing Group, 2020, 580 (7802), pp.269-273. ⟨10.1038/s41586-020-2080-8⟩
Nature, 580(7802), 269-273. Nature Publishing Group
Nature, Nature Publishing Group, 2020, 580 (7802), pp.269-273. ⟨10.1038/s41586-020-2080-8⟩
Nature, 580(7802), 269-273. Nature Publishing Group
International audience; Various species of the intestinal microbiota have been associated with the development of colorectal cancer(1,2), but it has not been demonstrated that bacteria have a direct role in the occurrence of oncogenic mutations. Esch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ea03f3d630c47a9677c25c397bbf599
https://doi.org/10.1038/s41586-020-2080-8
https://doi.org/10.1038/s41586-020-2080-8
Autor:
Sjoerd J. Klaasen, My Anh Truong, Richard H. van Jaarsveld, Isabella Koprivec, Valentina Štimac, Sippe G. de Vries, Patrik Risteski, Snježana Kodba, Kruno Vukušić, Kim L. de Luca, Joana F. Marques, Elianne M. Gerrits, Bjorn Bakker, Floris Foijer, Jop Kind, Iva M. Tolić, Susanne M. A. Lens, Geert J. P. L. Kops
Publikováno v:
Nature
Nature, 607, 604-609
Nature, 607, pp. 604-609
Nature, 607(7919), 604-609. Nature Publishing Group
Nature, 607, 604-609
Nature, 607, pp. 604-609
Nature, 607(7919), 604-609. Nature Publishing Group
Chromosome segregation errors during cell divisions generate aneuploidies and micronuclei, which can undergo extensive chromosomal rearrangements such as chromothripsis [1, 2, 3, 4, 5]. Selective pressures then shape distinct aneuploidy and rearrange
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::daaaba46aeec6daf7ebdc7fd431951dd
http://fulir.irb.hr/7580/
http://fulir.irb.hr/7580/
Autor:
Pieter S. Hiemstra, Evelien Eenjes, Irwin K M Reiss, Annelies M. Slats, Robbert J. Rottier, Sander van Riet, Hans Clevers, Dennis K. Ninaber, André A. Kroon, P. Padmini S.J. Khedoe, Marjon J Buscop-van Kempen, Anne Boerema-de Munck
Publikováno v:
American journal of physiology. Lung cellular and molecular physiology, 321(4), L775-L786. American Physiological Society
American Journal of Physiology-Lung Cellular and Molecular Physiology, 321(4), L775-L786. American Physiological Society
American Journal of Physiology-Lung Cellular and Molecular Physiology, 321(4), L775-L786. AMER PHYSIOLOGICAL SOC
American Journal of Physiology-Lung Cellular and Molecular Physiology, 321(4), L775-L786. American Physiological Society
American Journal of Physiology-Lung Cellular and Molecular Physiology, 321(4), L775-L786. AMER PHYSIOLOGICAL SOC
Air-liquid interface (ALI) cultures are frequently used in lung research but require substantial cell numbers that cannot readily be obtained from patients. We explored whether organoid expansion [three-dimensional (3D)] can be used to establish ALI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a14168f61ae2b61d818774ddd643a0aa
https://pure.knaw.nl/portal/en/publications/aaa00eea-135c-4217-a77c-7fb4306b005a
https://pure.knaw.nl/portal/en/publications/aaa00eea-135c-4217-a77c-7fb4306b005a
Autor:
D. Proverbio, T. Kralj, Madelon M. Maurice, Piet Gros, Sarah Stryeck, M. Goldflam, C. Ullman, G. Hermans, T. Aastrup, R.C. van Scherpenzeel, T.Z. Bass, Ingrid Jordens, Nicola Fenderico
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications
Nature Communications, 10(1), 365. Nature Publishing Group
'Nature Communications ', vol: 10, pages: 365-1-365-13 (2019)
Nature Communications
Nature Communications, 10(1), 365. Nature Publishing Group
'Nature Communications ', vol: 10, pages: 365-1-365-13 (2019)
Wnt-induced β-catenin-mediated transcription is a driving force for stem cell self-renewal during adult tissue homeostasis. Enhanced Wnt receptor expression due to mutational inactivation of the ubiquitin ligases RNF43/ZNRF3 recently emerged as a le
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76861a7482ab43d1a66ddb22e588929a
https://doaj.org/article/5a4f5bbea0514b429431ef3636e2ef04
https://doaj.org/article/5a4f5bbea0514b429431ef3636e2ef04
Autor:
Pirjo Nummela, Colin Nixon, William C. Clark, Ella Gilchrist, Simon J. Leedham, Michael C. Hodder, Nalle Pentinmikko, Arafath Kaja Najumudeen, Nathalie Sphyris, E. Yvonne Jones, Jean-Paul Vincent, Hans Clevers, Nicky J. Willis, Alexander Raven, Kristina Kirschner, Dustin J. Flanagan, Paul V. Fish, Emma Minnee, Pekka Katajisto, Ville Hietakangas, Owen J. Sansom, Christine Perret, Lynn McGarry, Nadia Nasreddin, Rachel A. Ridgway, Ann C. Williams, Kathryn Gilroy, Kalle Luopajärvi, Sandra Scharaw, Béatrice Romagnolo, Marianne Lähde, Ari Ristimäki, Johanna Englund, Anna Taylor Webb, Kari Alitalo, Ann Hedley
Publikováno v:
Nature, 594(7863), 430-435. Nature Publishing Group
Flanagan, D J, Williams, A C, Katajisto, P K, Sampson, O J & al., E 2021, ' NOTUM from Apc-mutant cells biases clonal competition to initiate cancer ', Nature, vol. 594, pp. 430–435 (2021) . https://doi.org/10.1038/s41586-021-03525-z
Flanagan, D J, Williams, A C, Katajisto, P K, Sampson, O J & al., E 2021, ' NOTUM from Apc-mutant cells biases clonal competition to initiate cancer ', Nature, vol. 594, pp. 430–435 (2021) . https://doi.org/10.1038/s41586-021-03525-z
Funding Information: Acknowledgements We thank the Core Services and Advanced Technologies at the Cancer Research UK Beatson Institute (C596/A17196 and A31287), and particularly the Biological Services Unit, Histology Service and Molecular Technologi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5733edf6d5fc44fd62aea7978eb694e
https://pure.knaw.nl/portal/en/publications/69a3582f-fc29-4f40-bc5f-17ba7825a11a
https://pure.knaw.nl/portal/en/publications/69a3582f-fc29-4f40-bc5f-17ba7825a11a
Autor:
Barbara Korzeniowska, Krzysztof Wrzesinski, Chrisna Gouws, Stephen J. Fey, Carlemi Calitz, Helle Sedighi Frandsen
Publikováno v:
Methods in Molecular Biology ISBN: 9781071612453
Wrzesinski, K, Frandsen, H S, Calitz, C, Gouws, C, Korzeniowska, B & Fey, S J 2021, Clinostat 3D Cell Culture : Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids . in T A L Brevini, A Fazeli & K Turksen (eds), Next Generation Culture Platforms for Reliable In Vitro Models : Methods and Protocols . Humana Press, Methods in Molecular Biology, vol. 2273, pp. 17-62 . https://doi.org/10.1007/978-1-0716-1246-0_2
Wrzesinski, K, Frandsen, H S, Calitz, C, Gouws, C, Korzeniowska, B & Fey, S J 2021, Clinostat 3D Cell Culture : Protocols for the Preparation and Functional Analysis of Highly Reproducible, Large, Uniform Spheroids and Organoids . in T A L Brevini, A Fazeli & K Turksen (eds), Next Generation Culture Platforms for Reliable In Vitro Models : Methods and Protocols . Humana Press, Methods in Molecular Biology, vol. 2273, pp. 17-62 . https://doi.org/10.1007/978-1-0716-1246-0_2
Growing cells as 3D structures need not be difficult. Often, it is not necessary to change cell type, additives or growth media used. All that needs to be changed is the geometry: cells (whether primary, induced pluripotent, transformed or immortal)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c0ab0bdffa4401be20952ab1b3b16ef
https://doi.org/10.1007/978-1-0716-1246-0_2
https://doi.org/10.1007/978-1-0716-1246-0_2
Autor:
Susana M. Chuva de Sousa Lopes, Sander J. Tans, Benedetta Artegiani, Xuan Zheng, Delilah F. G. Hendriks, Joep Beumer, Jeroen S. van Zon, Indi Joore, Rutger N.U. Kok, Hans Clevers
Publikováno v:
Nature Cell Biology, 22(3), 321-331. Nature Publishing Group
Nature Cell Biology, 22, 321-331. NATURE PUBLISHING GROUP
Nature Cell Biology, 22, 321-331. NATURE PUBLISHING GROUP
CRISPR-Cas9 technology has revolutionized genome editing and is applicable to the organoid field. However, precise integration of exogenous DNA sequences into human organoids is lacking robust knock-in approaches. Here, we describe CRISPR-Cas9-mediat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30c9a86ff1a9e9ce9fa4fbc7ba8103d8
https://hdl.handle.net/1887/3181947
https://hdl.handle.net/1887/3181947
Autor:
Susanne C. van den Brink, Judith Vivié, Alfonso Martinez Arias, Peter Baillie-Johnson, Anna Alemany, Katharina F. Sonnen, Marloes Blotenburg, Naomi Moris, Alexander van Oudenaarden, Jennifer Nichols, Vincent van Batenburg
Publikováno v:
Nature
Nature, 582(7812), 405-409. Nature Publishing Group
Nature, 582(7812), 405-409. Nature Publishing Group
Gastruloids are three-dimensional aggregates of embryonic stem cells that display key features of mammalian development after implantation, including germ-layer specification and axial organization1-3. To date, the expression pattern of only a small
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cb52f9df6d53d91a570baffcda59f670
Autor:
Edwin Cuppen, Nicolle Besselink, Valentina Sasselli, Roel Janssen, Sander Boymans, Hans Clevers, Myrthe Jager, Francis Blokzijl, Ruben van Boxtel
Publikováno v:
Nature Protocols, 13(1), 59-78. Nature Publishing Group
Nature Protocols, 13(1), 59. Nature Publishing Group
Nature Protocols, 13(1), 59. Nature Publishing Group
Characterization of mutational processes in adult stem cells (ASCs) will improve our understanding of aging-related diseases, such as cancer and organ failure, and may ultimately help prevent the development of these diseases. Here, we present a meth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5b95737006b922a6b98b66d8160152a3
https://doi.org/10.1038/nprot.2017.111
https://doi.org/10.1038/nprot.2017.111