Vliv sekvencí intronů na efektivitu sestřihu v Saccharomyces cerevisiae.

Autor: Oplová, Michaela

Pre-mRNA splicing is a highly regulated cellular process. The tight cooperation of spliceosome and other splicing factors that enable pre-mRNA cis-elements interpretation results in precise pre-mRNA splicing regulation. Short conserved splicing seque

Externí odkaz: http://www.nusl.cz/ntk/nusl-331225

Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport

Autor: Oborská-Oplová, Michaela, Fischer, Ute, Altvater, Martin, Panse, Vikram Govind

Publikováno v: Ribosome Biogenesis ISBN: 9781071625002
Methods Mol Biol

The process of eukaryotic ribosome assembly stretches across the nucleolus, the nucleoplasm and the cytoplasm, and therefore relies on efficient nucleocytoplasmic transport. In yeast, the import machinery delivers ~140,000 ribosomal proteins every mi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25a86bb3bd96651db07902c742dad8d3
https://doi.org/10.1007/978-1-0716-2501-9_7

Puf6 primes 60S pre-ribosome nuclear export at low temperature

Autor: Gerhardy, Stefan, Oborská-Oplová, Michaela, Gillet, Ludovic, Börner, Richard, van Nues, Rob, Leitner, Alexander, Michel, Erich, Petkowski, Janusz J., Granneman, Sander, Sigel, Roland K. O., Aebersold, Ruedi, Panse, Vikram Govind

Publikováno v: Gerhardy, S, Oborská-Oplová, M, Gillet, L, Börner, R, Van Nues, R, Leitner, A, Michel, E, Petkowski, J J, Granneman, S, Sigel, R K O, Aebersold, R & Panse, V G 2021, ' Puf6 primes 60S pre-ribosome nuclear export at low temperature ', Nature Communications, vol. 12, 4696 . https://doi.org/10.1038/s41467-021-24964-2
Nature Communications, 12 (1)
Nature Communications, Vol 12, Iss 1, Pp 1-16 (2021)
Nature Communications

Productive ribosomal RNA (rRNA) compaction during ribosome assembly necessitates establishing correct tertiary contacts between distant secondary structure elements. Here, we quantify the response of the yeast proteome to low temperature (LT), a cond

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5811aff3d0f227c38f85dfa8c9479c34
https://hdl.handle.net/20.500.11820/1e7b2b1f-4135-45bd-bd18-de14206f5054

The ribotoxin α-sarcin can cleave the sarcin/ricin loop on late 60S pre-ribosomes

Autor: Olombrada, Miriam, Peña, Cohue, Rodríguez-Galán, Olga, Klingauf-Nerurkar, Purnima, Portugal-Calisto, Daniela, Oborská-Oplová, Michaela, Altvater, Martin, Gavilanes, José G, Martínez-del-Pozo, Álvaro, de la Cruz, Jesús, García-Ortega, Lucía, Panse, Vikram Govind

Publikováno v: Digital.CSIC. Repositorio Institucional del CSIC
instname
E-Prints Complutense. Archivo Institucional de la UCM
E-Prints Complutense: Archivo Institucional de la UCM
Universidad Complutense de Madrid
Nucleic Acids Research
Nucleic Acids Research, 48 (11)

The ribotoxin α-sarcin belongs to a family of ribonucleases that cleave the sarcin/ricin loop (SRL), a critical functional rRNA element within the large ribosomal subunit (60S), thereby abolishing translation. Whether α-sarcin targets the SRL only

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::97f26ebc2009ae6b5367bbce6d8b2d58
https://doi.org/10.5167/uzh-190530

Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2

Autor: Schütz, Sabina, Michel, Erich, Damberger, Fred F, Oplová, Michaela, Peña, Cohue, Leitner, Alexander, Aebersold, Ruedi, Allain, Frederic H-T, Panse, Vikram Govind

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-14 (2018)
Nature Communications

Disordered extensions at the termini and short internal insertions distinguish eukaryotic ribosomal proteins (r-proteins) from their anucleated archaeal counterparts. Here, we report an NMR structure of such a eukaryotic-specific segment (ESS) in the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::7a7c3194b73c84de9fd5f44ac084e569
http://link.springer.com/article/10.1038/s41467-018-06160-x