An optimized method for plasma extracellular vesicles isolation to exclude the copresence of biological drugs and plasma proteins which impairs their biological characterization.

Autor: Onno J Arntz, Bartijn C H Pieters, Peter L E M van Lent, Marije I Koenders, Peter M van der Kraan, Fons A J van de Loo

Publikováno v: PLoS ONE, Vol 15, Iss 7, p e0236508 (2020)

Extracellular vesicles (EVs) are cell membrane-derived phospholipid bilayer nanostructures that contain bioactive proteins, enzymes, lipids and polymers of nucleotides. They play a role in intercellular communication and are present in body fluids. E

Externí odkaz: https://doaj.org/article/16f57ed371db47b689c9c415c50d4931

Commercial cow milk contains physically stable extracellular vesicles expressing immunoregulatory TGF-β.

Autor: Bartijn C H Pieters, Onno J Arntz, Miranda B Bennink, Mathijs G A Broeren, Arjan P M van Caam, Marije I Koenders, Peter L E M van Lent, Wim B van den Berg, Marieke de Vries, Peter M van der Kraan, Fons A J van de Loo

Publikováno v: PLoS ONE, Vol 10, Iss 3, p e0121123 (2015)

ScopeExtracellular vesicles, including exosomes, have been identified in all biological fluids and rediscovered as an important part of the intercellular communication. Breast milk also contains extracellular vesicles and the proposed biological func

Externí odkaz: https://doaj.org/article/f09e52a2843b43f2bebd39101869033a

Rheumatoid Arthritis Patients With Circulating Extracellular Vesicles Positive for IgM Rheumatoid Factor Have Higher Disease Activity

Autor: Onno J. Arntz, Bartijn C. H. Pieters, Rogier M. Thurlings, Mark H. Wenink, Peter L. E. M. van Lent, Marije I. Koenders, Frank H. J. van den Hoogen, Peter M. van der Kraan, Fons A. J. van de Loo

Publikováno v: Frontiers in Immunology, Vol 9 (2018)

Rheumatoid arthritis (RA) is an autoimmune inflammatory disease that mainly affects synovial joints. Validated laboratory parameters for RA diagnosis are higher blood levels of rheumatoid factor IgM (IgM-RF), anti-citrullinated protein autoantibodies

Externí odkaz: https://doaj.org/article/f63d323f91014ff290801a9c8a3e777a

Flood Control: How Milk-Derived Extracellular Vesicles Can Help to Improve the Intestinal Barrier Function and Break the Gut-Joint Axis in Rheumatoid Arthritis

Autor: Anouk L. Feitsma, Fons A. J. van de Loo, Jean Paul ten Klooster, Laura M’Rabet, Annemarie Boleij, Marije I. Koenders, R. J. Joost van Neerven, Onno J. Arntz, Bartijn C. H. Pieters, Joyce Aarts

Publikováno v: Frontiers in Immunology, 12
Frontiers in Immunology
Frontiers in Immunology 12 (2021)
Frontiers in Immunology, Vol 12 (2021)

Many studies provided compelling evidence that extracellular vesicles (EVs) are involved in the regulation of the immune response, acting as both enhancers and dampeners of the immune system, depending on the source and type of vesicle. Research, inc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0224e3b9fb615489d7125c73055d486f

Comparing Approaches to Normalize, Quantify, and Characterize Urinary Extracellular Vesicles

Autor: Omar A. Z. Tutakhel, Marian C. Clahsen-van Groningen, Cristian A. Carvajal, René J. M. Bindels, Thierry P P van den Bosch, David Severs, Juan Pablo Rigalli, Robert A. Fenton, Roger Carles-Fontana, Cathy A Cuevas, Joost G. J. Hoenderop, Charles J. Blijdorp, Ewout J. Hoorn, Martijn H van Heugten, Rob Willemsen, Fons A. J. van de Loo, Usha M. Musterd-Bhaggoe, Martin E. van Royen, Onno J. Arntz, Eric Barros, Thomas A Hartjes, Guido Jenster

Publikováno v: J Am Soc Nephrol
Blijdorp, C J, Tutakhel, O A Z, Hartjes, T A, van den Bosch, T P P, van Heugten, M H, Rigalli, J P, Willemsen, R, Musterd-Bhaggoe, U M, Barros, E R, Carles-Fontana, R, Carvajal, C A, Arntz, O J, van de Loo, F A J, Jenster, G, Clahsen-van Groningen, M C, Cuevas, C A, Severs, D, Fenton, R A, van Royen, M E, Hoenderop, J G J, Bindels, R J M & Hoorn, E J 2021, ' Comparing Approaches to Normalize, Quantify, and Characterize Urinary Extracellular Vesicles ', Journal of the American Society of Nephrology : JASN, vol. 32, no. 5, pp. 1210-1226 . https://doi.org/10.1681/ASN.2020081142
Journal of the American Society of Nephrology, 32, 1210-1226
Journal of the American Society of Nephrology, 32(5), 1210-1226. American Society of Nephrology
Journal of the American Society of Nephrology, 32, 5, pp. 1210-1226

Background Urinary extracellular vesicles (uEVs) are a promising source for biomarker discovery, but optimal approaches for normalization, quantification, and characterization in spot urines are unclear. Methods Urine samples were analyzed in a water

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1c0c64668a5a8ea0fb9f5169d71f5c4d
https://pubmed.ncbi.nlm.nih.gov/33782170