Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Onno D. Broekmans"'
Publikováno v:
eLife, Vol 5 (2016)
We exploit the reduced space of C. elegans postures to develop a novel tracking algorithm which captures both simple shapes and also self-occluding coils, an important, yet unexplored, component of 2D worm behavior. We apply our algorithm to show tha
Externí odkaz:
https://doaj.org/article/6638ba3373b1405bb203b51cf4e5be83
Autor:
Erwin J.G. Peterman, Onno D. Broekmans, Andreas S. Biebricher, Iddo Heller, Gerrit Sitters, Gijs J.L. Wuite
Publikováno v:
ChemPhysChem, 15, 727-733. Wiley-VCH Verlag
Heller, I, Sitters, G, Broekmans, O D, Biebricher, A S, Wuite, G J L & Peterman, E J G 2014, ' Mobility Analysis of Super-Resolved Proteins on Optically Stretched DNA: Comparing Imaging Techniques and Parameters ', ChemPhysChem, vol. 15, pp. 727-733 . https://doi.org/10.1002/cphc.201300813
Heller, I, Sitters, G, Broekmans, O D, Biebricher, A S, Wuite, G J L & Peterman, E J G 2014, ' Mobility Analysis of Super-Resolved Proteins on Optically Stretched DNA: Comparing Imaging Techniques and Parameters ', ChemPhysChem, vol. 15, pp. 727-733 . https://doi.org/10.1002/cphc.201300813
Fluorescence microscopy in conjunction with optical tweezers is well suited to the study of protein mobility on DNA. Here, we evaluate the benefits and drawbacks of super-resolution and conventional imaging techniques for the analysis of one-dimensio
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7a229779f88c4102a15362ac6db3a63c
https://hdl.handle.net/1871.1/6169a16c-166d-43ba-bfab-8192d75d4250
https://hdl.handle.net/1871.1/6169a16c-166d-43ba-bfab-8192d75d4250
Autor:
Iddo, Heller, Niels, Laurens, Daan, Vorselen, Onno D, Broekmans, Andreas S, Biebricher, Graeme A, King, Ineke, Brouwer, Gijs J L, Wuite, Erwin J G, Peterman
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 1486
Optical manipulation techniques provide researchers the powerful ability to directly move, probe and interrogate molecular complexes. Quadruple optical trapping is an emerging method for optical manipulation and force spectroscopy that has found its
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::ae64226b9fcc19a295c834b4d2c1dc6c
https://pubmed.ncbi.nlm.nih.gov/27844431
https://pubmed.ncbi.nlm.nih.gov/27844431
Autor:
Onno D. Broekmans, Niels Laurens, Graeme A. King, Iddo Heller, Ineke Brouwer, Erwin J.G. Peterman, Andreas S. Biebricher, Daan Vorselen, Gijs J.L. Wuite
Publikováno v:
Optical Tweezers ISBN: 9781493964192
Optical manipulation techniques provide researchers the powerful ability to directly move, probe and interrogate molecular complexes. Quadruple optical trapping is an emerging method for optical manipulation and force spectroscopy that has found its
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::55fd3e85049c924fdc4f4f093c24905c
https://doi.org/10.1007/978-1-4939-6421-5_9
https://doi.org/10.1007/978-1-4939-6421-5_9
Publikováno v:
Broekmans, O D, King, G A, Stephens, G J & Wuite, G J L 2016, ' DNA Twist Stability Changes with Magnesium(2+) Concentration ', Physical Review Letters, vol. 116, no. 25, 258102 . https://doi.org/10.1103/PhysRevLett.116.258102
Physical Review Letters, 116(25):258102. American Physical Society
Physical Review Letters, 116(25):258102. American Physical Society
For an understanding of DNA elasticity at high mechanical loads (F > 30 pN), its helical nature needs to be taken into account, in the form of coupling between the twist and stretch degrees of freedom. The prevailing model for DNA elasticity, the wor
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7ea490476566b32c0fc57d39fb80e837
https://doi.org/10.1103/physrevlett.116.258102
https://doi.org/10.1103/physrevlett.116.258102
Autor:
Iddo Heller, Géraldine Farge, Gerrit Sitters, Gijs J.L. Wuite, Carolin Menges, Wolfgang Wende, Onno D. Broekmans, Erwin J.G. Peterman, Stefan W. Hell
Publikováno v:
SPIE Proceedings.
Dense coverage of DNA by proteins is a ubiquitous feature of cellular processes such as DNA organization, replication and repair. We present a single-molecule approach capable of visualizing individual DNA-binding proteins on densely covered DNA and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::ac3ca954efb05977a524c121f24a54eb
https://doi.org/10.1117/12.2062819
https://doi.org/10.1117/12.2062819
Autor:
Siet M.J.L. van den Wildenberg, Niels Laurens, Onno D. Broekmans, Géraldine Farge, Maria Falkenberg, Erwin J.G. Peterman, Gijs J.L. Wuite, Martina Gaspari, Claes M. Gustafsson, Linda C.M. Dekker
Publikováno v:
Nature Communications
Nature Communications, 3:1013. Nature Publishing Group
NATURE COMMUNICATIONS
Farge, G A, Laurens, N, Broekmans, O D, Wildenberg, S M J L, Dekker, L C M, Gaspari, M, Gustafsson, C M, Peterman, E J G, Falkenberg, M & Wuite, G J L 2012, ' Protein sliding and DNA denaturation are essential for DNA organization by human mitochondrial transcription factor A ', Nature Communications, vol. 3, 1013 . https://doi.org/10.1038/ncomms2001
NATURE COMMUNICATIONS, 3, 1013
Nature Communications, 3:1013. Nature Publishing Group
NATURE COMMUNICATIONS
Farge, G A, Laurens, N, Broekmans, O D, Wildenberg, S M J L, Dekker, L C M, Gaspari, M, Gustafsson, C M, Peterman, E J G, Falkenberg, M & Wuite, G J L 2012, ' Protein sliding and DNA denaturation are essential for DNA organization by human mitochondrial transcription factor A ', Nature Communications, vol. 3, 1013 . https://doi.org/10.1038/ncomms2001
NATURE COMMUNICATIONS, 3, 1013
Mitochondria organize their genome in proteinĝ€"DNA complexes called nucleoids. The mitochondrial transcription factor A (TFAM), a protein that regulates mitochondrial transcription, is abundant in these nucleoids. TFAM is believed to be essential
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c0030b702f001138dfec83ea68ecc04
https://hdl.handle.net/1887/61372
https://hdl.handle.net/1887/61372
Publikováno v:
Nano letters. 9(8)
Solid-state nanopores offer a promising method for rapidly probing the structural properties of biopolymers such as DNA and RNA. We have for the first time translocated RNA molecules through solid-state nanopores, comparing the signatures of transloc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ea6502229fd00242dd06034ad20d3fe3
https://pubmed.ncbi.nlm.nih.gov/19537802
https://pubmed.ncbi.nlm.nih.gov/19537802
Autor:
Gijs J.L. Wuite, Linda C.M. Dekker, Géraldine Farge, Niels Laurens, Maria Falkenberg, Onno D. Broekmans, Erwin J.G. Peterman
Publikováno v:
Biophysical Journal. 98(3):221a-222a
Mitochondria are the energy producing organelles of eukaryotic cells. Owing to their endosymbiotic evolutionary history, they contain their own genome (mtDNA) that encodes for thirteen proteins essential for ATP production.In mammalian cells, multipl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e23f68c0277607b97de40c65e9e610b5