Výsledky vyhledávání - "Ondřej Mandula"

Alternation of inverse problem approach and deep learning for lens-free microscopy image reconstruction

Autor: Olivier Cioni, Mathilde Menneteau, Ondřej Mandula, Cédric Allier, Sophie Morales, Dorothee C. Kraemer, Lionel Hervé

Publikováno v: Scientific Reports
Scientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)

A lens-free microscope is a simple imaging device performing in-line holographic measurements. In the absence of focusing optics, a reconstruction algorithm is used to retrieve the sample image by solving the inverse problem. This is usually performe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e35343f13d8eff06e6a2eb0653c79bbc
https://doi.org/10.1038/s41598-020-76411-9

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Phase and fluorescence imaging with a surprisingly simple microscope based on chromatic aberration

Autor: Ondřej Mandula, Jean-Philippe Kleman, Cédric Allier, Françoise Lacroix, Sophie Morales, Pierre Blandin, Daniel Fiole, Dorothee C. Kraemer, Lionel Hervé

Publikováno v: Optics Express
Optics Express, 2020, 28 (2), pp.2079. ⟨10.1364/OE.28.002079⟩
Optics Express, Optical Society of America-OSA Publishing, 2020, 28 (2), pp.2079. ⟨10.1364/OE.28.002079⟩

We propose a simple and compact microscope combining phase imaging with multi-color fluorescence using a standard bright-field objective. The phase image of the sample is reconstructed from a single, approximately 100 μm out-of-focus image taken und

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::89ead49fa0c99a68a8977b31eb42fe9e
https://hal.univ-grenoble-alpes.fr/hal-02443413

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PyNX.Ptycho: a computing library for X-ray coherent diffraction imaging of nanostructures

Autor: Vincent Favre-Nicolin, Manfred Burghammer, Joël Eymery, Marta Elzo Aizarna, Ondřej Mandula

Publikováno v: Journal of Applied Crystallography
Journal of Applied Crystallography, International Union of Crystallography, 2016, 49 (5), pp.1842-1848. ⟨10.1107/s1600576716012279⟩
Journal of Applied Crystallography, 2016, 49 (5), pp.1842-1848. ⟨10.1107/s1600576716012279⟩

International audience; X-ray imaging techniques have undergone a remarkable development during the past decade, taking advantage of coherent X-ray sources. Among these techniques, ptychography allows reconstruction of the specimen and the illuminati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72b79cc1d650ce47a29e20c4e5019425
https://doi.org/10.1107/s1600576716012279

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Quantitative phase imaging of adherent mammalian cells: a comparative study

Autor: Pierre Blandin, Sophie Morales, Julien Savatier, Lionel Hervé, Yves Usson, Cédric Allier, Ondřej Mandula, Serge Monneret

Publikováno v: Biomedical optics express
Biomedical optics express, Optical Society of America-OSA Publishing, 2019, 10 (6), pp.2768. ⟨10.1364/BOE.10.002768⟩
Biomedical optics express, 2019, 10 (6), pp.2768. ⟨10.1364/BOE.10.002768⟩

International audience; The quantitative phase imaging methods have several advantages when it comes to monitoring cultures of adherent mammalian cells. Because of low photo-toxicity and no need for staining, we can follow cells in a minimally invasi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e84d91b8d6e197ba521054c68c34449a
https://doi.org/10.1364/boe.10.002768

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t-SNARE Protein Conformations Patterned by the Lipid Microenvironment*

Autor: Rory R. Duncan, Silvio O. Rizzoli, Ondřej Mandula, David Moulton, Colin Rickman, Nagaraj D. Halemani, Claire N. Medine, Luke H. Chamberlain, Alison R. Dun

Publikováno v: The Journal of Biological Chemistry
Rickman, C, Medine, C N, Dun, A R, Moulton, D J, Mandula, O, Halemani, N D, Rizzoli, S O, Chamberlain, L H & Duncan, R R 2010, ' t-SNARE Protein Conformations Patterned by the Lipid Microenvironment ', Journal of Biological Chemistry, vol. 285, no. 18, pp. 13535-13541 . https://doi.org/10.1074/jbc.M109.091058

The spatial distribution of the target (t-) SNARE proteins (syntaxin and SNAP-25) on the plasma membrane has been extensively characterized. However, the protein conformations and interactions of the two t-SNAREs in situ remain poorly defined. By usi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16dc9b5e2b18973a9475b7c2ac26327b
http://europepmc.org/articles/PMC2859514

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Localisation microscopy with quantum dots using non-negative matrix factorisation

Autor: Ivana Šumanovac Šestak, Rainer Heintzmann, Ondřej Mandula, Christopher Williams

Publikováno v: Mandula, O, Sestak, I S, Heintzmann, R & Williams, C K I 2014, ' Localisation microscopy with quantum dots using non-negative matrix factorisation ', Optics Express, vol. 22, no. 20, pp. 24594-24605 . https://doi.org/10.1364/OE.22.024594

We propose non-negative matrix factorisation with iterative restarts (iNMF) to model a noisy dataset of highly overlapping fluorophores with intermittent intensities. We can recover high-resolution images of individual sources from the optimised mode

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab5655b2606550ac9d315d9afb5de688
https://pubmed.ncbi.nlm.nih.gov/25322035

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Akademický článek

CNN-Based Cell Analysis: From Image to Quantitative Representation

Autor: Cédric Allier, Lionel Hervé, Chiara Paviolo, Ondrej Mandula, Olivier Cioni, William Pierré, Francesca Andriani, Kiran Padmanabhan, Sophie Morales

Publikováno v: Frontiers in Physics, Vol 9 (2022)

We present a novel deep learning-based quantification pipeline for the analysis of cell culture images acquired by lens-free microscopy. The image reconstruction part of the pipeline features a convolutional neural network performing phase unwrapping

Externí odkaz: https://doaj.org/article/07bb527288714231b881379abe15d3a5

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Akademický článek

Ultrafast and Multiplexed Bacteriophage Susceptibility Testing by Surface Plasmon Resonance and Phase Imaging of Immobilized Phage Microarrays

Autor: Larry O'Connell, Ondrej Mandula, Loïc Leroy, Axelle Aubert, Pierre R. Marcoux, Yoann Roupioz

Publikováno v: Chemosensors, Vol 10, Iss 5, p 192 (2022)

In the context of bacteriophage (phage) therapy, there is an urgent need for a method permitting multiplexed, parallel phage susceptibility testing (PST) prior to the formulation of personalized phage cocktails for administration to patients sufferin

Externí odkaz: https://doaj.org/article/43f6dec932e44092bb74ae90bd31dbb6

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