Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Ondřej Bernatík"'
Autor:
Lucia Binó, Erika Mikulenková, Luděk Štepánek, Ondřej Bernatík, David Vysloužil, Petra Pejšková, Peter Gorilák, Martina Huranová, Vladimír Varga, Lukáš Čajánek
Publikováno v:
STAR Protocols, Vol 3, Iss 1, Pp 101199- (2022)
Summary: Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generatio
Externí odkaz:
https://doaj.org/article/82221d426ce0417bb69be54d3c6da585
Autor:
Kateřina Hanáková, Ondřej Bernatík, Marek Kravec, Miroslav Micka, Jitender Kumar, Jakub Harnoš, Petra Ovesná, Petra Paclíková, Matěj Rádsetoulal, David Potěšil, Konstantinos Tripsianes, Lukáš Čajánek, Zbyněk Zdráhal, Vítězslav Bryja
Publikováno v:
Cell Communication and Signaling, Vol 17, Iss 1, Pp 1-21 (2019)
Abstract Background Dishevelled (DVL) is an essential component of the Wnt signaling cascades. Function of DVL is controlled by phosphorylation but the molecular details are missing. DVL3 contains 131 serines and threonines whose phosphorylation gene
Externí odkaz:
https://doaj.org/article/59bcf25501ed4898813e2a7a806d2bac
Autor:
Jakub Harnoš, Maria Consuelo Alonso Cañizal, Miroslav Jurásek, Jitender Kumar, Cornelia Holler, Alexandra Schambony, Kateřina Hanáková, Ondřej Bernatík, Zbyněk Zdráhal, Kristína Gömöryová, Tomáš Gybeľ, Tomasz Witold Radaszkiewicz, Marek Kravec, Lukáš Trantírek, Jan Ryneš, Zankruti Dave, Ana Iris Fernández-Llamazares, Robert Vácha, Konstantinos Tripsianes, Carsten Hoffmann, Vítězslav Bryja
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-18 (2019)
Dishevelled (DVL) is the key component of Wnt signaling pathway that acts as a signaling hub. Here, authors study the conformational dynamics of DVL3 in vivo using their engineered FlAsH-based FRET biosensors and describe how Wnt activation opens DVL
Externí odkaz:
https://doaj.org/article/c656ec6d84244ea4ba47e39232d91995
The initiation of assembly of primary cilia, organelles with crucial functions in development and disease, is under the control of Tau-Tubulin Kinase 2 (TTBK2). Recent work has implicated TTBK2 also in the regulation of primary cilia maintenance and
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::abdbd8ea6f7ffbbf4a90e51a99771cfe
https://doi.org/10.1101/2023.02.21.529351
https://doi.org/10.1101/2023.02.21.529351
Autor:
Miroslav Micka, Matěj Rádsetoulal, Kateřina Hanáková, Petra Paclíková, Jakub Harnoš, Ondřej Bernatík, Petra Ovesná, Lukas Cajanek, Konstantinos Tripsianes, David Potěšil, Jitender Kumar, Zbyněk Zdráhal, Marek Kravec, Vítězslav Bryja
Publikováno v:
Cell Communication and Signaling : CCS
Cell Communication and Signaling, Vol 17, Iss 1, Pp 1-21 (2019)
Cell Communication and Signaling, Vol 17, Iss 1, Pp 1-21 (2019)
BackgroundDishevelled (DVL) is an essential component of the Wnt signaling cascades. Function of DVL is controlled by phosphorylation but the molecular details are missing. DVL3 contains 131 serines and threonines whose phosphorylation generates comp
Autor:
David Potěšil, Vítězslav Bryja, Igor Cervenka, Kateřina Hanáková, Matěj Rádsetoulal, Ondřej Bernatík, Zbyněk Zdráhal
Publikováno v:
Hungarian Journal of Industry and Chemistry, Vol 46, Iss 1, Pp 3-6 (2018)
The focus of this paper is the human Dishevelled 3 protein (hDvl3), an essential component of the Wnt signalling pathway that contributes to their regulation. Mass spectrometry-based analysis of hDvl3 phosphorylations induced by eight associated kina
Autor:
Alexandra Schambony, Tomasz Witold Radaszkiewicz, Maria Consuelo Alonso Cañizal, Robert Vácha, Jan Ryneš, Konstantinos Tripsianes, Jitender Kumar, Carsten Hoffmann, Zbyněk Zdráhal, Kateřina Hanáková, Zankruti Dave, Lukáš Trantírek, Miroslav Jurásek, Ana Iris Fernández-Llamazares, Jakub Harnoš, Marek Kravec, Cornelia Holler, Ondřej Bernatík, Vítězslav Bryja, Tomáš Gybeľ, Kristína Gömöryová
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-18 (2019)
Nature Communications
Nature Communications
Dishevelled (DVL) is the key component of the Wnt signaling pathway. Currently, DVL conformational dynamics under native conditions is unknown. To overcome this limitation, we develop the Fluorescein Arsenical Hairpin Binder- (FlAsH-) based FRET in v
Autor:
Kateřina Hanáková, Ondřej Bernatík, Kravec, Marek, Micka, Miroslav, Jitender Kumar, Harnoš, Jakub, Ovesná, Petra, Paclíková, Petra, Matěj Rádsetoulal, Potěšil, David, Tripsianes, Konstantinos, Lukáš Čajánek, Zbyněk Zdráhal, Vítězslav Bryja
Additional file 4: Figure S4. NMR comparison of DVL2 PDZ wild-type and phosphorylation-mimicking mutant S281E + S298E. A. 1H, 15 N HSQC spectra of PDZ wild-type (blue) and phosphorylation-mimicking mutant S281E + S298E (red). All amide frequencies ha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::783d6f433c0fbe9b78b83a79fc072b0f
Autor:
Harnos, Jakub, Cañizal, Maria Consuelo Alonso, Jurásek, Miroslav, Jitender Kumar, Holler, Cornelia, Schambony, Alexandra, Kateřina Hanáková, Ondřej Bernatík, Zbyněk Zdráhal, Gömöryová, Kristína, Tomáš Gybeľ, Radaszkiewicz, Tomasz, Kravec, Marek, Lukáš Trantírek, Ryneš, Jan, Dave, Zankruti, Fernández, Ana Iris, Vácha, Robert, Tripsianes, Konstantinos, Hoffmann, Carsten, Vítězslav Bryja
Main figures 1-9 from the manuscript.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9adcae8c970970d6430e0a795a6898a5
Publikováno v:
Molecular and Cellular Biology
Dishevelled (DVL) proteins are key mediators of the Wnt/β-catenin signaling pathway. All DVL proteins contain three conserved domains: DIX, PDZ, and DEP. There is a consensus in the field that the DIX domain is critical for Wnt/β-catenin signaling,