A novel therapeutic with two SNAP-25 inactivating proteases shows long-lasting anti-hyperalgesic activity in a rat model of neuropathic pain

Autor: Om Prakash Edupuganti, Orla Moriarty, Jianghui Meng, John Nealon, Jiafu Wang, Laura Casals-Diaz, Oliver James Dolly, Tomas H. Zurawski

Publikováno v: Neuropharmacology. 118:223-232

A pressing need exists for long-acting, non-addictive medicines to treat chronic pain, a major societal burden. Botulinum neurotoxin type A (BoNT/A) complex – a potent, specific and prolonged inhibitor of neuro-exocytosis – gives some relief in s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2ee784f01a5b5f0599b6799aac142ff7
https://doi.org/10.1016/j.neuropharm.2017.03.026

Biological and synthetic binders for immunoassay and sensor-based detection: generation and characterisation of an anti-AFB2 single-chain variable fragment (scFv)

Autor: Richard O'Kennedy, Om Prakash Edupuganti, Soujanya Ratna Edupuganti

Publikováno v: World Mycotoxin Journal. 6:273-280

Aflatoxins are highly carcinogenic in nature and occur in a variety of food and feed samples. To ensure food is free from aflatoxins and safe for human consumption, methods to detect these toxins are necessary. Both aflatoxin B1 and aflatoxin B2 (AFB

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c573216f01e9ef7d4d7e1a13e00c145a
https://doi.org/10.3920/wmj2012.1523

Use of T-2 toxin-immobilized amine-activated beads as an efficient affinity purification matrix for the isolation of specific IgY

Autor: Stéphanie Boullanger, Om Prakash Edupuganti, Richard O'Kennedy, Eric Defrancq, Soujanya Ratna Edupuganti

Publikováno v: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2013, pp.98-101
Europe PubMed Central

An affinity purification method that isolates T-2 toxin-specific IgY utilizing a T-2-toxin-immobilized column was developed. The T-2 toxin was covalently coupled via a carbonyldiimidazole-activated hydroxyl functional group to amine-activated sepharo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c063dd5e7ce92dde328c36445220a64c
https://hal.archives-ouvertes.fr/hal-00825418

A highly stable, sensitive, regenerable and rapid immunoassay for detecting aflatoxin B1 in corn incorporating covalent AFB1 immobilization and a recombinant Fab antibody

Autor: Stephen Hearty, Om Prakash Edupuganti, Richard O'Kennedy, Soujanya Ratna Edupuganti

Publikováno v: Talanta. 115

A highly robust immunoassay applicable for the detection of aflatoxin B1 (AFB1) using a Fab antibody fragment was developed. A key factor was the use of covalently immobilized AFB1 which allowed an almost three fold increase in sensitivity, reduced a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bece5a1799ba48455ee3546ffbf93179
https://pubmed.ncbi.nlm.nih.gov/24054599

Covalent bonding of vancomycin to Ti6Al4V alloy pins provides long-term inhibition of Staphylococcus aureus colonization

Autor: Noreen J. Hickok, Samuel B. King, Valentin Antoci, Binoy Jose, Javad Parvizi, Eric Wickstrom, Om Prakash Edupuganti, Irving M. Shapiro, Allen R. Zeiger, Christopher S. Adams

Publikováno v: Bioorganicmedicinal chemistry letters. 17(10)

Self-protecting Ti6Al4V alloy pins were prepared by covalent bonding of bis(ethylene glycol) linkers, then vancomycin to the oxidized, aminopropylated Ti6Al4V alloy surface. Fluorescence modification-enabled estimation of yields of free amines on the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a8073fd499051234f2ea0e817164267
https://pubmed.ncbi.nlm.nih.gov/17369042