Zobrazeno 1 - 10
of 101
pro vyhledávání: '"Olivier Hyrien"'
Autor:
Bertrand Theulot, Laurent Lacroix, Jean-Michel Arbona, Gael A. Millot, Etienne Jean, Corinne Cruaud, Jade Pellet, Florence Proux, Magali Hennion, Stefan Engelen, Arnaud Lemainque, Benjamin Audit, Olivier Hyrien, Benoît Le Tallec
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
Theulot et al. introduce NanoForkSpeed, a nanopore sequencing-based method to map individual replication fork velocities on entire genomes. NFS shows that fork speed is uniform across yeast chromosomes except for a marked slowdown at pausing sites.
Externí odkaz:
https://doaj.org/article/b8e2f80d57284f0b8386e98104ca5aad
Autor:
Rachel A. DeWeerd, Eszter Németh, Ádám Póti, Nataliya Petryk, Chun-Long Chen, Olivier Hyrien, Dávid Szüts, Abby M. Green
Publikováno v:
Cell Reports, Vol 38, Iss 12, Pp 110555- (2022)
Summary: Mutational signatures defined by single base substitution (SBS) patterns in cancer have elucidated potential mutagenic processes that contribute to malignancy. Two prevalent mutational patterns in human cancers are attributed to the APOBEC3
Externí odkaz:
https://doaj.org/article/0976eac5cc3d4734bc831e3d308df896
Autor:
Magali Hennion, Jean-Michel Arbona, Laurent Lacroix, Corinne Cruaud, Bertrand Theulot, Benoît Le Tallec, Florence Proux, Xia Wu, Elizaveta Novikova, Stefan Engelen, Arnaud Lemainque, Benjamin Audit, Olivier Hyrien
Publikováno v:
Genome Biology, Vol 21, Iss 1, Pp 1-25 (2020)
Abstract Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200-nucleotide resolution. By quantifying
Externí odkaz:
https://doaj.org/article/207b69b66bc4499fb532f23fff185faf
Autor:
Nina Kirstein, Alexander Buschle, Xia Wu, Stefan Krebs, Helmut Blum, Elisabeth Kremmer, Ina M Vorberg, Wolfgang Hammerschmidt, Laurent Lacroix, Olivier Hyrien, Benjamin Audit, Aloys Schepers
Publikováno v:
eLife, Vol 10 (2021)
Eukaryotic DNA replication initiates during S phase from origins that have been licensed in the preceding G1 phase. Here, we compare ChIP-seq profiles of the licensing factors Orc2, Orc3, Mcm3, and Mcm7 with gene expression, replication timing, and f
Externí odkaz:
https://doaj.org/article/44e8901c580f40a8879fd1df94d25925
Autor:
Nataliya Petryk, Malik Kahli, Yves d'Aubenton-Carafa, Yan Jaszczyszyn, Yimin Shen, Maud Silvain, Claude Thermes, Chun-Long Chen, Olivier Hyrien
Publikováno v:
Nature Communications, Vol 7, Iss 1, Pp 1-13 (2016)
The physical origin and termination sites of DNA replication in human cells have remained elusive. Here the authors use Okazaki fragment sequencing to reveal global replication patterns and show how chromatin and transcription modulate the process.
Externí odkaz:
https://doaj.org/article/33be268cd5f4463da7c86414495060a0
Publikováno v:
eLife, Vol 7 (2018)
The time-dependent rate [Formula: see text] of origin firing per length of unreplicated DNA presents a universal bell shape in eukaryotes that has been interpreted as the result of a complex time-evolving interaction between origins and limiting firi
Externí odkaz:
https://doaj.org/article/3c251189e5da4515b9c1ff99bf76398f
Publikováno v:
Cell Reports, Vol 3, Iss 5, Pp 1629-1639 (2013)
c-Myc oncogenic activity is thought to be mediated in part by its ability to generate DNA replication stress and subsequent genomic instability when deregulated. Previous studies have demonstrated a nontranscriptional role for c-Myc in regulating DNA
Externí odkaz:
https://doaj.org/article/3a7a2ffab33f482e86f588485605ce60
Autor:
Olivier Hyrien
Publikováno v:
F1000Research, Vol 5 (2016)
DNA replication origins strikingly differ between eukaryotic species and cell types. Origins are localized and can be highly efficient in budding yeast, are randomly located in early fly and frog embryos, which do not transcribe their genomes, and ar
Externí odkaz:
https://doaj.org/article/9b87c2bce0b94eccbe5da21d1d154712
Autor:
Hélène Labit, Arach Goldar, Guillaume Guilbaud, Carine Douarche, Olivier Hyrien, Kathrin Marheineke
Publikováno v:
BioTechniques, Vol 45, Iss 6, Pp 649-658 (2008)
Molecular combing of DNA is an extremely powerful DNA fiber–stretching technique that is often used in DNA replication and genome stability studies. Optimal DNA combing results mainly depend on the quality of the silanized surfaces onto which fiber
Externí odkaz:
https://doaj.org/article/8a77db124600439a9290e93f214e0b33
Autor:
Antoine Baker, Benjamin Audit, Chun-Long Chen, Benoit Moindrot, Antoine Leleu, Guillaume Guilbaud, Aurélien Rappailles, Cédric Vaillant, Arach Goldar, Fabien Mongelard, Yves d'Aubenton-Carafa, Olivier Hyrien, Claude Thermes, Alain Arneodo
Publikováno v:
PLoS Computational Biology, Vol 8, Iss 4, p e1002443 (2012)
In higher eukaryotes, replication program specification in different cell types remains to be fully understood. We show for seven human cell lines that about half of the genome is divided in domains that display a characteristic U-shaped replication
Externí odkaz:
https://doaj.org/article/7d11a983c8a84058b54c32765c749815