Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Olivier Borkowski"'
Publikováno v:
Computational and Structural Biotechnology Journal, Vol 21, Iss , Pp 3173-3182 (2023)
Because they mimic cells while offering an accessible and controllable environment, lysate-based cell-free systems (CFS) have emerged as valuable biotechnology tools for synthetic biology. Historically used to uncover fundamental mechanisms of life,
Externí odkaz:
https://doaj.org/article/0df8859590514277b78993368160c7db
Autor:
Olivier Borkowski, Mathilde Koch, Agnès Zettor, Amir Pandi, Angelo Cardoso Batista, Paul Soudier, Jean-Loup Faulon
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
Cell-free lysates are a major platform for in vitro protein production but batch-to-batch variation makes production difficult to predict. Here the authors develop an active learning approach to optimising buffer conditions to bring homemade lysates
Externí odkaz:
https://doaj.org/article/94a3b8e52cf24f769347e3c91609b690
Autor:
Olivier Borkowski, Carlos Bricio, Michela Murgiano, Brooke Rothschild-Mancinelli, Guy-Bart Stan, Tom Ellis
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
The translation of heterologous proteins places a burden on host cell resources, affecting growth and productivity. Here the authors develop a cell-free assay to measure resource consumption and predict in vivo burden.
Externí odkaz:
https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
Autor:
Olivier Borkowski, Anne Goelzer, Marc Schaffer, Magali Calabre, Ulrike Mäder, Stéphane Aymerich, Matthieu Jules, Vincent Fromion
Publikováno v:
Molecular Systems Biology, Vol 12, Iss 5, Pp 1-14 (2016)
Abstract Complex regulatory programs control cell adaptation to environmental changes by setting condition‐specific proteomes. In balanced growth, bacterial protein abundances depend on the dilution rate, transcript abundances and transcript‐spec
Externí odkaz:
https://doaj.org/article/d59fef70fd714eb08d535f88d57ba3c0
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 6 (2018)
Cell-free TX-TL is an increasingly mature and useful platform for prototyping, testing, and engineering biological parts and systems. However, to fully accomplish the promises of synthetic biology, mathematical models are required to facilitate the d
Externí odkaz:
https://doaj.org/article/7b5ec44a128f4291914b5b4fb9b2f874
Autor:
Ali Samy Abdelaal, David Adam, Hiyam Adil Altaii, Zhong-Hu Bai, Upasana Basu, Gargi Bhattacharjee, Jorge Bolívar, Olivier Borkowski, Siqin Cai, Pablo Carbonell, François Coutte, Matthieu Da Costa, Teresa de Diego Puente, Debarun Dhali, Manuel Cánovas Díaz, Christoph Diehl, Carrie A. Eckert, Diego E. Egoburo, Noura El-Ahmady El-Naggar, Francisco J. Enguita, Emily F. Freed, Julia Gallego-Jara, Nisarg Gohil, Ragaa A. Hamouda, Philippe Jacques, M. Julia Pettinari, Pannaga P. Jutur, Mukul S. Kareya, Valérie Leclère, Ana Lúcia Leitão, Ye Li, Liya Liang, Rongming Liu, Xiu-Xia Liu, Zengxin Ma, Catherine Madzak, Rosa Alba Sola Martínez, Ryosuke Mitsui, Xuhua Mo, Charles Moritz, Justyna Mozejko-Ciesielska, Asha A. Nesamma, Chetan Paliwal, Amir Pandi, Subha Sankar Paul, Ahmad Bazli Ramzi, Lennart Schada von Borzyskowski, Rabinder Singh, Vijai Singh, Sean Stettner, Yuman Sun, Srividhya Sundaram, Gema Lozano Terol, Heykel Trabelsi, Antonio Valle, Mengying Wang, Han Xiao, Xinhui Xing, Ryosuke Yamada, Song Yang, Yazen Yaseen, Syed Shams Yazdani, Guihong Yu, Changtai Zhang, Hui Zhang
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::bdc76169f6b2c065d506daf89abde7a6
https://doi.org/10.1016/b978-0-12-821477-0.09995-5
https://doi.org/10.1016/b978-0-12-821477-0.09995-5
Autor:
Olivier Borkowski, Charles Moritz, Amir Pandi, Christoph Diehl, David Adam, Srividhya Sundaram
Cell-free systems are in vitro platforms to perform biotransformation and to test biological pathways beyond cell boundaries. These platforms either are supplied by purified enzymes or contain proteins performing transcription and translation in vitr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4d06fa1739d21be9ccfe29a0eaa4ed86
https://doi.org/10.1016/b978-0-12-821477-0.00008-8
https://doi.org/10.1016/b978-0-12-821477-0.00008-8
Publikováno v:
Advances in Synthetic Biology
Advances in Synthetic Biology, Springer Singapore, pp. 275-288, 2020, ⟨10.1007/978-981-15-0081-7_16⟩
Advances in Synthetic Biology ISBN: 9789811500800
Advances in Synthetic Biology, Springer Singapore, pp. 275-288, 2020, ⟨10.1007/978-981-15-0081-7_16⟩
Advances in Synthetic Biology ISBN: 9789811500800
International audience; Cell-free systems are emerging as membraneless transcriptional and translational tools to study biology and for prototyping, characterization, and engineering novel biological systems. Cell-free systems allow in vitro transcri
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2b85f031e6e4a7a29fdd79e8c570891
https://hal.science/hal-03852064
https://hal.science/hal-03852064
Autor:
Guy-Bart Stan, Olivier Borkowski, Tom Ellis, Michela Murgiano, Brooke Rothschild-Mancinelli, Carlos Bricio Garberi
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-11 (2018)
Nature Communications
Nature Communications
Translating heterologous proteins places significant burden on host cells, consuming expression resources leading to slower cell growth and productivity. Yet predicting the cost of protein production for any given gene is a major challenge, as multip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::151f6217852b2c770871740d0316aa79
https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
https://doaj.org/article/30d5c258a0ca46a1b23db0978ae952b8
Autor:
Paul Soudier, Mathilde Koch, Angelo Cardoso Batista, Amir Pandi, Jean-Loup Faulon, Agnès Zettor, Olivier Borkowski
Lysate-based cell-free systems have become a major platform to study gene expression but batch-to-batch variation makes protein production difficult to predict. Here we describe an active learning approach to explore a combinatorial space of ~4,000,0
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::499e3809f5de693471823669637e2fc5
https://doi.org/10.1101/751669
https://doi.org/10.1101/751669