A safety cap protects hydrogenase from oxygen attack

Autor: Martin Winkler, Jifu Duan, Andreas Rutz, Christina Felbek, Lisa Scholtysek, Oliver Lampret, Jan Jaenecke, Ulf-Peter Apfel, Gianfranco Gilardi, Francesca Valetti, Vincent Fourmond, Eckhard Hofmann, Christophe Léger, Thomas Happe

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)

[FeFe]-hydrogenases catalyze the conversion of protons and electrons to molecular hydrogen, but upon exposure to oxygen, their catalytic cofactor is irreversibly inactivated. Here, the authors determine the crystal structure of hydrogenase CbA5H and

Externí odkaz: https://doaj.org/article/9c9745d6547b4e9c8c0781318c4c1e61

The roles of long-range proton-coupled electron transfer in the directionality and efficiency of [FeFe]-hydrogenases

Autor: Oliver Lampret, Martin Winkler, Jifu Duan, Thomas Happe, Fraser A. Armstrong, Eckhard Hofmann

Publikováno v: Proc Natl Acad Sci U S A

As paradigms for proton-coupled electron transfer in enzymes and benchmarks for a fully renewable H(2) technology, [FeFe]-hydrogenases behave as highly reversible electrocatalysts when immobilized on an electrode, operating in both catalytic directio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01bd67c6ad7b28c65ef2454960bca486
https://doi.org/10.1073/pnas.2007090117

A safety cap protects hydrogenase from oxygen attack

Autor: Lisa Scholtysek, Ulf-Peter Apfel, Vincent Fourmond, Christina Felbek, Gianfranco Gilardi, Oliver Lampret, Jan Jaenecke, Francesca Valetti, Jifu Duan, Thomas Happe, Eckhard Hofmann, Andreas Rutz, Martin Winkler, Christophe Léger

Publikováno v: Nature Communications
Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-020-20861-2⟩
'Nature Communications ', vol: 12, pages: 756-1-756-10 (2021)
Nature Communications, Nature Publishing Group, 2021, 12 (1), ⟨10.1038/s41467-020-20861-2⟩
Nature Communications, Vol 12, Iss 1, Pp 1-10 (2021)

[FeFe]-hydrogenases are efficient H2-catalysts, yet upon contact with dioxygen their catalytic cofactor (H-cluster) is irreversibly inactivated. Here, we combine X-ray crystallography, rational protein design, direct electrochemistry, and Fourier-tra

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f172a71e483de8388502ba850657f08
https://hal.science/hal-03129104

The final steps of [FeFe]-hydrogenase maturation

Autor: Rieke Haas, Julian Esselborn, Oliver Lampret, Leonie Kertess, Clare F. Megarity, Thomas Happe, Rosalind L. Booth, Martin Winkler, Fraser A. Armstrong, Andreas Rutz, Florian Wittkamp

Publikováno v: Lampret, O, Esselborn, J, Haas, R, Rutz, A, Booth, R L, Kertess, L, Wittkamp, F, Megarity, C F, Armstrong, F A, Winkler, M & Happe, T 2019, ' The final steps of [FeFe]-hydrogenase maturation ', Proceedings of the National Academy of Sciences of the United States of America, vol. 116, no. 32, pp. 15802-15810 . https://doi.org/10.1073/pnas.1908121116

The active site (H-cluster) of [FeFe]-hydrogenases is a blueprint for the design of a biologically inspired H2-producing catalyst. The maturation process describes the preassembly and uptake of the unique [2FeH] cluster into apo-hydrogenase, which is

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::719110fef4668045fef59ac169de6986
https://doi.org/10.1073/pnas.1908121116

Interplay between CN

Autor: Oliver, Lampret, Agnieszka, Adamska-Venkatesh, Hannes, Konegger, Florian, Wittkamp, Ulf-Peter, Apfel, Edward J, Reijerse, Wolfgang, Lubitz, Olaf, Rüdiger, Thomas, Happe, Martin, Winkler

Publikováno v: Journal of the American Chemical Society. 139(50)

The catalytic cofactor of [FeFe]-hydrogenses (H-cluster) is composed of a generic cubane [4Fe-4S]-cluster (4Fe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6ddb9cd64928c8776b037451e069e2a8
https://pubmed.ncbi.nlm.nih.gov/29179539