How to alleviate cardiac injury from electric shocks at the cellular level

Autor: Pamela W. Sowa, Aleksander S. Kiełbik, Andrei G. Pakhomov, Emily Gudvangen, Uma Mangalanathan, Volker Adams, Olga N. Pakhomova

Publikováno v: Frontiers in Cardiovascular Medicine, Vol 9 (2022)

Electric shocks, the only effective therapy for ventricular fibrillation, also electroporate cardiac cells and contribute to the high-mortality post-cardiac arrest syndrome. Copolymers such as Poloxamer 188 (P188) are known to preserve the membrane i

Externí odkaz: https://doaj.org/article/358634790a3b4390b8c7673860881b99

The conserved adenosine in helix 6 of Archaeoglobus fulgidus signal recognition particle RNA initiates SRP assembly

Autor: Jiaming Yin, Qiaojia Huang, Olga N. Pakhomova, Andrew P. Hinck, Christian Zwieb

Publikováno v: Archaea, Vol 1, Iss 4, Pp 269-275 (2004)

The signal recognition particle (SRP) RNA helix 6 of archaea and eukaryotes is essential for the binding of protein SRP19 and the assembly of a functional complex. The conserved adenosine at the third position of the tetraloop of helix 6 (A149) is cr

Externí odkaz: https://doaj.org/article/e6c3305a3fbc4ca193faad9184ede9a5

Cell Electrosensitization Exists Only in Certain Electroporation Buffers.

Autor: Janja Dermol, Olga N Pakhomova, Andrei G Pakhomov, Damijan Miklavčič

Publikováno v: PLoS ONE, Vol 11, Iss 7, p e0159434 (2016)

Electroporation-induced cell sensitization was described as the occurrence of a delayed hypersensitivity to electric pulses caused by pretreating cells with electric pulses. It was achieved by increasing the duration of the electroporation treatment

Externí odkaz: https://doaj.org/article/5952efe560d24f9da9089303cce0bb7c

The role of ESCRT-III and Annexin V in the repair of cell membrane permeabilization by the nanosecond pulsed electric field

Autor: Giedre Silkuniene, Claudia Muratori, Peter A. Mollica, Andrei G. Pakhomov, Olga N. Pakhomova

Publikováno v: Bioelectrochemistry (Amsterdam, Netherlands). 140

Exposure of living cells to intense nanosecond pulsed electric field (nsPEF) increases membrane permeability to small solutes, presumably by the formation of nanometer-size membrane lesions. Mechanisms responsible for the restoration of membrane inte

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cfafa9985eb0974db03cbc6d991fc2b2
https://pubmed.ncbi.nlm.nih.gov/34004548

Probing Nanoelectroporation and Resealing of the Cell Membrane by the Entry of Ca2+ and Ba2+ Ions

Autor: Olga N. Pakhomova, Maura Casciola, Uma Mangalanathan, Wenfei Bo, Shu Xiao, Mantas Silkunas, Andrei G. Pakhomov, Iurii Semenov, Vitalij Novickij

Publikováno v: International Journal of Molecular Sciences, Vol 21, Iss 3386, p 3386 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 9
International journal of molecular sciences, Basel : MDPI, 2020, vol. 21, 3386, p. 2-17

The principal bioeffect of the nanosecond pulsed electric field (nsPEF) is a lasting cell membrane permeabilization, which is often attributed to the formation of nanometer-sized pores. Such pores may be too small for detection by the uptake of fluor

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c7c15942bbd24f94957391701f428d
http://dspace.vgtu.lt/handle/1/3989