Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Olga Musharova"'
Autor:
Dmitry Sutormin, Alina Galivondzhyan, Olga Musharova, Dmitrii Travin, Anastasiia Rusanova, Kseniya Obraztsova, Sergei Borukhov, Konstantin Severinov
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-19 (2022)
In E. coli, disruption of TopoI and RNAP interaction decreases cells viability and leads to hypernegative DNA supercoiling and R loops accumulation. TopoI and DNA gyrase bind around transcription units and TopoI recognizes cleavage sites by a specifi
Externí odkaz:
https://doaj.org/article/f5466d64289f4cadad858d1bc28b877d
Autor:
Olga Musharova, Danylo Vyhovskyi, Sofia Medvedeva, Jelena Guzina, Yulia Zhitnyuk, Marko Djordjevic, Konstantin Severinov, Ekaterina Savitskaya
Publikováno v:
mBio, Vol 9, Iss 6 (2018)
ABSTRACT CRISPR DNA arrays of unique spacers separated by identical repeats ensure prokaryotic immunity through specific targeting of foreign nucleic acids complementary to spacers. New spacers are acquired into a CRISPR array in a process of CRISPR
Externí odkaz:
https://doaj.org/article/7aa6c4b730ee47aeb0dc8159091991c9
Autor:
Anna A. Shiriaeva, Konstantin Kuznedelov, Ivan Fedorov, Olga Musharova, Timofey Khvostikov, Yuliya Tsoy, Elena Kurilovich, Gerald R. Smith, Ekaterina Semenova, Konstantin Severinov
Publikováno v:
Science Advances. 8
CRISPR-Cas systems provide prokaryotes with adaptive immunity against foreign nucleic acids. In Escherichia coli , immunity is acquired upon integration of 33-bp spacers into CRISPR arrays. DNA targets complementary to spacers get degraded and serve
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::dce91136e21180c55ce4cc17327a241d
https://doi.org/10.1126/sciadv.abn8650
https://doi.org/10.1126/sciadv.abn8650
Autor:
Maksim Kazalov, Mikhail Khodorkovskii, Anatolii Arseniev, Polina Selkova, Sergey Shmakov, T. O. Artamonova, Aleksandra Vasileva, Daria Artamonova, Olga Musharova, Ignatiy Goryanin, Tatyana Zyubko, Konstantin Severinov, Iana Fedorova, Georgii Pobegalov, Marina Abramova
Publikováno v:
Nucleic Acids Research
CRISPR-Cas defense systems opened up the field of genome editing due to the ease with which effector Cas nucleases can be programmed with guide RNAs to access desirable genomic sites. Type II-A SpCas9 from Streptococcus pyogenes was the first Cas9 nu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::18c51d476fa75cbb9b79cbfc30b7453f
https://doi.org/10.1093/nar/gkaa998
https://doi.org/10.1093/nar/gkaa998
Position of Deltaproteobacteria Cas12e nuclease cleavage sites depends on spacer length of guide RNA
Autor:
Maksim Kazalov, Konstantin Severinov, Nataliia Shcheglova, Aleksandra Vasileva, Polina Selkova, T. O. Artamonova, Iana Fedorova, Tatyana Zyubko, Anatolii Arseniev, Georgii Pobegalov, Olga Musharova, Mikhail Khodorkovskii
Publikováno v:
RNA Biology
article-version (VoR) Version of Record
article-version (VoR) Version of Record
Cas12e proteins (formerly CasX) form a distinct subtype of Class II type V CRISPR-Cas effectors. Recently, it was shown that DpbCas12e from Deltaproteobacteria and PlmCas12e from Planctomycetes can introduce programmable double-stranded breaks in mam
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e91779ee9c44ab505ddd1ff75715ba13
http://europepmc.org/articles/PMC7549622
http://europepmc.org/articles/PMC7549622
Autor:
Marina Abramova, Daria Artamonova, Ignatiy Goryanin, Olga Musharova, Mikhail Khodorkovskii, Konstantin Severinov, Sergey Shmakov, Georgii Pobegalov, Polina Selkova, T. O. Artamonova, Tatyana Zyubko, Maksim Kazalov, Aleksandra Vasileva, Anatolii Arseniev, Iana Fedorova
Publikováno v:
Nucleic Acids Research
Type II CRISPR–Cas9 RNA-guided nucleases are widely used for genome engineering. Type II-A SpCas9 protein from Streptococcus pyogenes is the most investigated and highly used enzyme of its class. Nevertheless, it has some drawbacks, including a rel
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9adf1dfe831092ce6078be606074b64
https://doi.org/10.1093/nar/gkz1225
https://doi.org/10.1093/nar/gkz1225
Autor:
Ekaterina Semenova, Konstantin Severinov, Andrey Krivoy, Kirill A. Datsenko, Ivan Fedorov, Vasily Sitnik, Marnix Vlot, Olga Musharova, Stan J. J. Brouns, Ekaterina Savitskaya
Publikováno v:
Molecular Microbiology, 111(6)
Molecular Microbiology
Molecular Microbiology 111 (2019) 6
Molecular Microbiology, 111(6), 1558-1570
Molecular Microbiology
Molecular Microbiology 111 (2019) 6
Molecular Microbiology, 111(6), 1558-1570
Summary CRISPR interference occurs when a protospacer recognized by the CRISPR RNA is destroyed by Cas effectors. In Type I CRISPR‐Cas systems, protospacer recognition can lead to «primed adaptation» – acquisition of new spacers from in cis loc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7d4d0ed9374f60bb4445ab5c2289dc5
https://doi.org/10.1111/mmi.14237
https://doi.org/10.1111/mmi.14237
Autor:
Ekaterina Savitskaya, Anna Shiriaeva, Olga Musharova, Evgeny Klimuk, Noemí M. Guzmán, Ekaterina Semenova, Sofia Medvedeva, Victor G. Zgoda, Konstantin Severinov, Daria Titova
Publikováno v:
Proceedings of the National Academy of Sciences of the United States of America
Significance Primed adaptation allows rapid acquisition of protective spacers derived from foreign mobile genetic elements into CRISPR arrays of the host. Primed adaptation requires ongoing CRISPR interference that destroys foreign genetic elements,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::42bc4633a27fe4c3715a1d9ed8c593ac
https://doi.org/10.1073/pnas.2021291118
https://doi.org/10.1073/pnas.2021291118
Autor:
Anastasia Metlitskaya, Evgeny Klimuk, Kirill A. Datsenko, Konstantin Severinov, Maria D. Logacheva, Ekaterina Semenova, Olga Musharova, Ekaterina Savitskaya
Publikováno v:
Nucleic Acids Research
During primed CRISPR adaptation spacers are preferentially selected from DNA recognized by CRISPR interference machinery, which in the case of Type I CRISPR–Cas systems consists of CRISPR RNA (crRNA) bound effector Cascade complex that locates comp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76b5a36d8e9c474dcd5142eefbcc0d99
http://europepmc.org/articles/PMC5389516
http://europepmc.org/articles/PMC5389516
Autor:
Danylo Vyhovskyi, Sofia Medvedeva, Konstantin Severinov, Ekaterina Savitskaya, Olga Musharova, Yulia Zhitnyuk, Jelena Guzina, Marko Djordjevic
Publikováno v:
mBio, Vol 9, Iss 6 (2018)
mBio, Vol 9, Iss 6, p e02169-18 (2018)
mBio
mBio, Vol 9, Iss 6, p e02169-18 (2018)
mBio
Adaptive immunity of prokaryotes depends on acquisition of foreign DNA fragments into CRISPR arrays as spacers followed by destruction of foreign DNA by CRISPR interference machinery. Different fragments are acquired into CRISPR arrays with widely di
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afec8d42051d4d40dbda7425a5569a2a
https://journals.asm.org/doi/10.1128/mBio.02169-18
https://journals.asm.org/doi/10.1128/mBio.02169-18