Cavin1 intrinsically disordered domains are essential for fuzzy electrostatic interactions and caveola formation

Autor: Vikas A. Tillu, James Rae, Ya Gao, Nicholas Ariotti, Matthias Floetenmeyer, Oleksiy Kovtun, Kerrie-Ann McMahon, Natasha Chaudhary, Robert G. Parton, Brett M. Collins

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021)

Caveolae are spherical nanodomains of the plasma membrane generated by assembly of caveolin and cavin proteins. Here, the authors show that fuzzy electrostatic interactions between caveolin-1 and Cavin1 proteins, combined with membrane lipid interact

Externí odkaz: https://doaj.org/article/2807bb40181f4244bd067fd1cb562ab3

FCHO controls AP2’s critical endocytic roles through a PtdIns4,5P2 membrane-dependent switch

Autor: Nathan R. Zaccai, Zuzana Kadlecova, Veronica Kane Dickson, Kseniya Korobchevskaya, Jan Kamenicky, Oleksiy Kovtun, Perunthottathu K. Umasankar, Antoni G. Wrobel, Jonathan G.G. Kaufman, Sally Gray, Kun Qu, Philip R. Evans, Marco Fritzsche, Filip Sroubek, Stefan Höning, John A.G. Briggs, Bernard T. Kelly, David J. Owen, Linton M. Traub

Clathrin-mediated endocytosis (CME) is the main mechanism by which mammalian cells control their cell surface proteome. Proper operation of the pivotal CME cargo-adaptor AP2 requires membrane-localised FCHO. Here, live-cell eTIRF-SIM shows that FCHO

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e79a12d731600e38e491e1c3decb394d
https://doi.org/10.1101/2022.04.02.486817

Single-molecule analysis reveals self assembly and nanoscale segregation of two distinct cavin subcomplexes on caveolae

Autor: Yann Gambin, Nicholas Ariotti, Kerrie-Ann McMahon, Michele Bastiani, Emma Sierecki, Oleksiy Kovtun, Mark E Polinkovsky, Astrid Magenau, WooRam Jung, Satomi Okano, Yong Zhou, Natalya Leneva, Sergey Mureev, Wayne Johnston, Katharina Gaus, John F Hancock, Brett M Collins, Kirill Alexandrov, Robert G Parton

Publikováno v: eLife, Vol 3 (2014)

In mammalian cells three closely related cavin proteins cooperate with the scaffolding protein caveolin to form membrane invaginations known as caveolae. Here we have developed a novel single-molecule fluorescence approach to directly observe interac

Externí odkaz: https://doaj.org/article/3222892030ff4bd78593681b9cee2d10

Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes

Autor: Marie-Kristin von Wrisberg, Juri Rappsilber, Olga Perisic, Roger L. Williams, Sarah L. Maslen, Dustin R. Morado, John A. G. Briggs, Kathrin Lang, Jessie Bertram, Shirley Tremel, Sean Munro, Mark Skehel, Oleksiy Kovtun, Yohei Ohashi, Zhuo A. Chen, Laura T L Brandt

Publikováno v: Nature Communications, 12
Tremel, S, Ohashi, Y, Morado, D R, Bertram, J, Perisic, O, Brandt, L T L, von Wrisberg, M K, Chen, Z A, Maslen, S L, Kovtun, O, Skehel, M, Rappsilber, J, Lang, K, Munro, S, Briggs, J A G & Williams, R L 2021, ' Structural basis for VPS34 kinase activation by Rab1 and Rab5 on membranes ', Nature Communications, vol. 12, 1564 . https://doi.org/10.1038/s41467-021-21695-2
Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)
Nature Communications

The lipid phosphatidylinositol-3-phosphate (PI3P) is a regulator of two fundamental but distinct cellular processes, endocytosis and autophagy, so its generation needs to be under precise temporal and spatial control. PI3P is generated by two complex

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f817ee0df381ae701383613316007446
https://hdl.handle.net/20.500.11850/529518

Architecture of the AP2:clathrin coat on the membranes of clathrin-coated vesicles

Autor: John A. G. Briggs, Bernard T. Kelly, Veronica Kane Dickson, Oleksiy Kovtun, David J. Owen

Publikováno v: Science Advances

Cryo-electron tomography reveals the conformational changes and clathrin interactions of AP2 during coated vesicle assembly.
Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell’s plasma membrane. Cla

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c2abc3e7c790ff33b9b65e574679be61
https://doi.org/10.1101/2020.01.28.922591