The enzyme and the cDNA sequence of a thermolabile and double-strand specific DNase from Northern shrimps (Pandalus borealis).

Autor: Inge W Nilsen, Kersti Øverbø, Linda Jensen Havdalen, Morten Elde, Dag Rune Gjellesvik, Olav Lanes

Publikováno v: PLoS ONE, Vol 5, Iss 4, p e10295 (2010)

BACKGROUND: We have previously isolated a thermolabile nuclease specific for double-stranded DNA from industrial processing water of Northern shrimps (Pandalus borealis) and developed an application of the enzyme in removal of contaminating DNA in PC

Externí odkaz: https://doaj.org/article/525c647dc26041eea40a8b3fb69ceaa5

Going to extremes – a metagenomic journey into the dark matter of life

Autor: Alexandra Helleux, Håkon Dahle, Alexander Sczyrba, Karolina Kwiatkowska-Semrau, Anders Svensson, Ruoshi Zhang, Paul Terzian, Olesia Werbowy, Edda Olgudóttir, Gudmundur O. Hreggvidsson, Jonathan Vincent, Andrius Jasilionis, David Prangishvili, Thibaud Mas, Ehmke Pohl, Javier A. Linares-Pastén, Tobias Lutterman, Olafur H. Fridjonsson, Agata Jurczak-Kurek, Martin Steinegger, Arnthor Aevarsson, Justine Vanhalst, Mart Krupovic, Elisabet E. Gudmundsdottir, Sigurlaug Skirnisdottir, Milot Mirdita, Tadeusz Kaczorowski, Monika Szadkowska, Joanna Lange, Magdalena Plotka, Hildegard Watzlawick, François Enault, Magdalena Cichowicz-Cieślak, Sigmar K. Stefansson, Björn Walse, Jessica Louise Ray, Steffen A Lorentsen, Jörn Kalinowski, Slawomir Dabrowski, Josefin Ahlqvist, William Merre, Eric Olo Ndela, Lei Wang, Salam Al-Karadaghi, Sebastian Dorawa, Martin Welin, Francine Perler, Cathrine Pedersen, Tara Róbertsdóttir, Stefanie Freitag-Pohl, Anna-Karina Kaczorowska, Ruth-Anne Sandaa, Jérémy Courtin, Ilmur Jónsdóttir, Ewa Wons, Bjorn T. Adalsteinsson, Úlfur Áugúst Átlasson, Birkir Reynisson, Katrine Stange Overå, Christian Henke, Maria Håkansson, Julien Lebrat, Annika Jochheim, Mathilde Tourigny, Bas Vroling, Ying Liu, Eirin Glomsaker, Olav Lanes, Sigrídur Hjörleifdóttir, Sólveig K Pétursdóttir, Lukasz P. Kozlowski, Bernd Striberny, David Brandt, Agnieszka Morzywolek, Mickael Guérin, Julia Dusaucy, Ida Helene Steen, Clovis Galiez, Sigurd E Gundesø, J. Altenbuchner, Lilja Björk Jónsdóttir, Jørn Remi Henriksen, Katy A. S. Cornish, Tom van den Bergh, Eva Nordberg Karlsson, Johannes Söding, Hördur Gudmundsson, Hasan Arsin, Anita-Elin Fedøy, Emma J Tarrant, Samia Djeffane, Terese Solstad

Publikováno v: FEMS Microbiology Letters
FEMS Microbiology Letters, 2021, 368 (12), pp.fnab067. ⟨10.1093/femsle/fnab067⟩

The Virus-X-Viral Metagenomics for Innovation Value-project was a scientific expedition to explore and exploit uncharted territory of genetic diversity in extreme natural environments such as geothermal hot springs and deep-sea ocean ecosystems. Spec

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c5ace0dc1df1a5c289378af4e9b0cf30
https://hal-pasteur.archives-ouvertes.fr/pasteur-03698255

Optimisation of the surface electrostatics as a strategy for cold adaptation of uracil-DNA N-glycosylase (UNG) from Atlantic cod (Gadus morhua)

Autor: Olav Lanes, Ingar Leiros, Magne Olufsen, Nils Peeler Willassen, Ellen Kristin Riise, Ame O. Smalas, Elin Moe

Publikováno v: Journal of molecular biology. 343(5)

Cold-adapted enzymes are characterised by an increased catalytic efficiency and reduced temperature stability compared to their mesophilic counterparts. Lately, it has been suggested that an optimisation of the electrostatic surface potential is a st

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::15c1055facfac06ba0d6693d8c972f1f
https://pubmed.ncbi.nlm.nih.gov/15491608

Identification, cloning, and expression of uracil-DNA glycosylase from Atlantic cod (Gadus morhua): characterization and homology modeling of the cold-active catalytic domain

Autor: Olav Lanes, Nils Peder Willassen, Ingar Leiros, Arne O. Smalås

Publikováno v: Extremophiles : life under extreme conditions. 6(1)

Two distinct forms of the highly conserved uracil-DNA glycosylase (UNG) have been isolated from Atlantic cod (Gadus morhua) liver cDNA by rapid amplification of cDNA ends (RACE). From the cDNA sequences, both forms were deduced to encode an open read

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a64786487a06b6a5279f1a925afe5f7
https://pubmed.ncbi.nlm.nih.gov/11878565

Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua)

Autor: Nils Peder Willassen, Dag Rune Gjellesvik, Olav Lanes, Per Henrik Guddal

Publikováno v: Comparative biochemistry and physiology. Part B, Biochemistrymolecular biology. 127(3)

Uracil-DNA glycosylase (UDG; UNG) has been purified 17000-fold from Atlantic cod liver (Gadus morhua). The enzyme has an apparent molecular mass of 25 kDa, as determined by gel filtration, and an isoelectric point above 9.0. Atlantic cUNG is inhibite

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::46064a296a82425ca5c7a2da46f6e38a
https://pubmed.ncbi.nlm.nih.gov/11126771