A conserved immunogenic and vulnerable site on the coronavirus spike protein delineated by cross-reactive monoclonal antibodies

Autor: Chunyan Wang, Rien van Haperen, Javier Gutiérrez-Álvarez, Wentao Li, Nisreen M. A. Okba, Irina Albulescu, Ivy Widjaja, Brenda van Dieren, Raul Fernandez-Delgado, Isabel Sola, Daniel L. Hurdiss, Olalekan Daramola, Frank Grosveld, Frank J. M. van Kuppeveld, Bart L. Haagmans, Luis Enjuanes, Dubravka Drabek, Berend-Jan Bosch

Publikováno v: Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021)

Here, the authors report the isolation and characterization of two human monoclonal antibodies (mAbs) from immunized mice with trimeric spike ectodomains of three human betacoronaviruses HCoV-OC43, SARS-CoV and MERSCoV, and show that while exhibiting

Externí odkaz: https://doaj.org/article/db6da7f5d9bb4e1c916d69bc00d5ab39

CHO synthetic promoters improve expression and product quality of biotherapeutic proteins

Autor: Si Nga Sou, Claire L. Harris, Rebecca Williams, Dorota Kozub, Fabio Zurlo, Yash D. Patel, Praveen Kallamvalli Illam Sankaran, Olalekan Daramola, Adam Brown, David C. James, Diane Hatton, Sarah Dunn, Suzanne J. Gibson

Publikováno v: Biotechnology Progress.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::088b3dd34516a35eafaed5112c57e28d
https://doi.org/10.1002/btpr.3348

A platform for context-specific genetic engineering of recombinant protein production by CHO cells

Autor: Claire L. Arnall, Sarah Dunn, Nicholas O. W. Barber, Ray Field, Joseph F. Cartwright, David C. James, Andrew A. Peden, Diane Hatton, Adam J. Brown, Olalekan Daramola, Clare S. Lovelady, Guglielmo Rosignoli, Claire Harris, Suzanne J. Gibson, Greg Dean, Yash D. Patel

Publikováno v: Journal of Biotechnology. 312:11-22

An increasing number of engineered therapeutic recombinant proteins with unpredictable manufacturability are currently filling industrial cell line development pipelines. These proteins can be "difficult-to-express" (DTE) in that production of a suff

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e73353cda98397f3952e3480b93b9bd
https://doi.org/10.1016/j.jbiotec.2020.02.012

Multimeric single-domain antibody complexes protect against bunyavirus infections

Autor: Frank Grosveld, Sara Rodriguez Conde, Paul J. Wichgers Schreur, Dorota Kozub, Jan Hellert, Kieran K Mistry, Erick Bermúdez-Méndez, Martin Beer, Pablo Guardado-Calvo, Félix A. Rey, Karen Brennan, Ziyan Deng, Michiel M. Harmsen, Olalekan Daramola, Sandra G. P. van de Water, Jeroen Kortekaas, Dubravka Drabek, Maiken Søndergaard Kristiansen, Andrea Aebischer, Lucien van Keulen, Kerstin Wernike

Publikováno v: eLife
eLife, eLife Sciences Publication, 2020, 9, pp.e52716. ⟨10.7554/eLife.52716⟩
eLife, 9:e52716. eLife Sciences Publications
eLife, 2020, 9, pp.e52716. ⟨10.7554/eLife.52716⟩
eLife, Vol 9 (2020)
eLife 9 (2020)
eLife, 9

International audience; The World Health Organization has included three bunyaviruses posing an increasing threat to human health on the Blueprint list of viruses likely to cause major epidemics and for which no, or insufficient countermeasures exist

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::efa19d5508677dc13a105be694ea4493
https://hal-pasteur.archives-ouvertes.fr/pasteur-03207969/file/elife-52716-v1.pdf

Polysome profiling of mAb producing CHO cell lines links translational control of cell proliferation and recombinant mRNA loading onto ribosomes with global and recombinant protein synthesis

Autor: Charlotte L, Godfrey, Emma J, Mead, Olalekan, Daramola, Sarah, Dunn, Diane, Hatton, Ray, Field, Gary, Pettman, C Mark, Smales

Publikováno v: Biotechnology journal. 12(8)

mRNA translation is a key process determining growth, proliferation and duration of a Chinese hamster ovary (CHO) cell culture and influences recombinant protein synthesis rate. During bioprocessing, CHO cells can experience stresses leading to repro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a1677dea3e02ec08da12bc8c20779c9f
https://pubmed.ncbi.nlm.nih.gov/28504349