One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections.

Autor: Janire Mingo, Asier Erramuzpe, Sandra Luna, Olaia Aurtenetxe, Laura Amo, Ibai Diez, Jan T G Schepens, Wiljan J A J Hendriks, Jesús M Cortés, Rafael Pulido

Publikováno v: PLoS ONE, Vol 11, Iss 8, p e0160972 (2016)

Site-directed mutagenesis (SDM) is a powerful tool to create defined collections of protein variants for experimental and clinical purposes, but effectiveness is compromised when a large number of mutations is required. We present here a one-tube-onl

Externí odkaz: https://doaj.org/article/04d9e549bcc447738dfc7af4f1ca7945

Dual-Specificity Phosphatases in Neuroblastoma Cell Growth and Differentiation

Autor: Olaia Aurtenetxe, Caroline E. Nunes-Xavier, José I. López, Ricardo López-Almaraz, Laura Zaldumbide, Rafael Pulido

Publikováno v: International Journal of Molecular Sciences
Addi. Archivo Digital para la Docencia y la Investigación
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International Journal of Molecular Sciences, Vol 20, Iss 5, p 1170 (2019)

Dual-specificity phosphatases (DUSPs) are important regulators of neuronal cell growth and differentiation by targeting proteins essential to neuronal survival in signaling pathways, among which the MAP kinases (MAPKs) stand out. DUSPs include the MA

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a5aa0c35faae1be9e00451c0b606ef4
http://hdl.handle.net/10810/32894

Tailor-Made Protein Tyrosine Phosphatases: In Vitro Site-Directed Mutagenesis of PTEN and PTPRZ-B

Autor: Sandra, Luna, Janire, Mingo, Olaia, Aurtenetxe, Lorena, Blanco, Laura, Amo, Jan, Schepens, Wiljan J, Hendriks, Rafael, Pulido

Publikováno v: Methods in molecular biology (Clifton, N.J.). 1447

In vitro site-directed mutagenesis (SDM) of protein tyrosine phosphatases (PTPs) is a commonly used approach to experimentally analyze PTP functions at the molecular and cellular level and to establish functional correlations with PTP alterations fou

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::8cfdde6c841359ffa524ac275d75bd79
https://pubmed.ncbi.nlm.nih.gov/27514801

One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections

Autor: Sandra Luna, Ibai Diez, Asier Erramuzpe, Wiljan Hendriks, Jesus M. Cortes, Olaia Aurtenetxe, Laura Amo, Janire Mingo, Jan Schepens, Rafael Pulido

Publikováno v: PLoS One, 11
PLoS ONE, Vol 11, Iss 8, p e0160972 (2016)
PLoS One, 11, 8
PLoS ONE

Contains fulltext : 171086.PDF (Publisher’s version ) (Open Access) Site-directed mutagenesis (SDM) is a powerful tool to create defined collections of protein variants for experimental and clinical purposes, but effectiveness is compromised when a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bda103f69dfee52a9e43bd4a367ab64
http://hdl.handle.net/2066/171086

A prospective biological study in relation to a family with Li-Fraumeni syndrome

Autor: Aurora Navajas, Pedro Perez, Begoña Calvo, Ana Fernández-Teijeiro, Olaia Aurtenetxe Sáez

Publikováno v: Clinical and Translational Oncology. 14:396-398

The Li-Fraumeni syndrome (LFS) is an autosomal dominant hereditary disorder associated with different tumor types in childhood and young adults. Approximately 70% of LFS cases contain germline mutations in the TP53 gene. We report a case of a family

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ab77f2174d7ef0d6901ac61e8bb0b443
https://doi.org/10.1007/s12094-012-0815-1