Zobrazeno 1 - 10
of 46
pro vyhledávání: '"Oksana V. Nekrasova"'
Autor:
Nikita A. Orlov, Elena V. Kryukova, Anastasia V. Efremenko, Sergey A. Yakimov, Victoria A. Toporova, Mikhail P. Kirpichnikov, Oksana V. Nekrasova, Alexey V. Feofanov
Publikováno v:
Membranes, Vol 13, Iss 7, p 645 (2023)
The voltage-gated potassium channel Kv1.1, which is abundant in the CNS and peripheral nervous system, controls neuronal excitability and neuromuscular transmission and mediates a number of physiological functions in non-excitable cells. The developm
Externí odkaz:
https://doaj.org/article/cfb44605bfc640008fec3610b2320174
Autor:
Alexandra L. Primak, Nikita A. Orlov, Steve Peigneur, Jan Tytgat, Anastasia A. Ignatova, Kristina R. Denisova, Sergey A. Yakimov, Mikhail P. Kirpichnikov, Oksana V. Nekrasova, Alexey V. Feofanov
Publikováno v:
Toxins, Vol 15, Iss 3, p 229 (2023)
The growing interest in potassium channels as pharmacological targets has stimulated the development of their fluorescent ligands (including genetically encoded peptide toxins fused with fluorescent proteins) for analytical and imaging applications.
Externí odkaz:
https://doaj.org/article/98a6a2fd89be41559e83da7da2cff6be
Autor:
Nikita A. Orlov, Anastasia A. Ignatova, Elena V. Kryukova, Sergey A. Yakimov, Mikhail P. Kirpichnikov, Oksana V. Nekrasova, Alexey V. Feofanov
Publikováno v:
Toxins, Vol 14, Iss 12, p 858 (2022)
The voltage-gated potassium Kv1.3 channel is an essential component of vital cellular processes which is also involved in the pathogenesis of some autoimmune, neuroinflammatory and oncological diseases. Pore blockers of the Kv1.3 channel are consider
Externí odkaz:
https://doaj.org/article/2905dd7e5aa7467a9870eac99fe0995e
Autor:
Kristina R. Denisova, Nikita A. Orlov, Sergey A. Yakimov, Mikhail P. Kirpichnikov, Alexey V. Feofanov, Oksana V. Nekrasova
Publikováno v:
Bioengineering, Vol 9, Iss 7, p 295 (2022)
Fluorescently labeled peptide blockers of ion channels are useful probes in studying the localization and functioning of the channels and in the performance of a search for new channel ligands with bioengineering screening systems. Here, we report on
Externí odkaz:
https://doaj.org/article/fe12d2e85cd942369de5dcec0d938c27
GFP–Margatoxin, a Genetically Encoded Fluorescent Ligand to Probe Affinity of Kv1.3 Channel Blockers
Autor:
Kristina R. Denisova, Nikita A. Orlov, Sergey A. Yakimov, Elena A. Kryukova, Dmitry A. Dolgikh, Mikhail P. Kirpichnikov, Alexey V. Feofanov, Oksana V. Nekrasova
Publikováno v:
International Journal of Molecular Sciences, Vol 23, Iss 3, p 1724 (2022)
Peptide pore blockers and their fluorescent derivatives are useful molecular probes to study the structure and functions of the voltage-gated potassium Kv1.3 channel, which is considered as a pharmacological target in the treatment of autoimmune and
Externí odkaz:
https://doaj.org/article/d4dbe515d42645eabc0ed9e54453a53f
Autor:
George V. Sharonov, Oksana V. Nekrasova, Ksenia S. Kudryashova, Mikhail P. Kirpichnikov, Alexey V. Feofanov
Publikováno v:
Bioengineering, Vol 8, Iss 11, p 187 (2021)
Screening drug candidates for their affinity and selectivity for a certain binding site is a crucial step in developing targeted therapy. Here, we created a screening assay for receptor binding that can be easily scaled up and automated for the high
Externí odkaz:
https://doaj.org/article/d16d354f7ef944c1a6ecd7667f7027b7
Autor:
Oksana V. Nekrasova, Alexandra L. Primak, Anastasia A. Ignatova, Valery N. Novoseletsky, Olga V. Geras’kina, Ksenia S. Kudryashova, Sergey A. Yakimov, Mikhail P. Kirpichnikov, Alexander S. Arseniev, Alexey V. Feofanov
Publikováno v:
Toxins, Vol 12, Iss 12, p 802 (2020)
Recently developed fluorescent protein-scorpion toxin chimeras (FP-Tx) show blocking activities for potassium voltage-gated channels of Kv1 family and retain almost fully pharmacological profiles of the parental peptide toxins (Kuzmenkov et al., Sci
Externí odkaz:
https://doaj.org/article/6c6e8f2d8c2143f7a9303d4be758b138
Autor:
Feofanov, Nikita A. Orlov, Elena V. Kryukova, Anastasia V. Efremenko, Sergey A. Yakimov, Victoria A. Toporova, Mikhail P. Kirpichnikov, Oksana V. Nekrasova, Alexey V.
Publikováno v:
Membranes; Volume 13; Issue 7; Pages: 645
The voltage-gated potassium channel Kv1.1, which is abundant in the CNS and peripheral nervous system, controls neuronal excitability and neuromuscular transmission and mediates a number of physiological functions in non-excitable cells. The developm
Autor:
M. A. Yakovleva, Mikhail A. Ostrovsky, Oksana V. Nekrasova, Victor A. Nadtochenko, Dmitry A. Cherepanov, Fedor E. Gostev, Ivan V. Shelaev, Irina B. Kolchugina, Lada E. Petrovskaya, T. B. Feldman, Dmitry A. Dolgikh, O. A. Smitienko, Mikhail P. Kirpichnikov
Publikováno v:
The Journal of Physical Chemistry B. 125:995-1008
The primary stages of the Exiguobacterium sibiricum rhodopsin (ESR) photocycle were investigated by femtosecond absorption laser spectroscopy in the spectral range of 400-900 nm with a time resolution of 25 fs. The dynamics of the ESR photoreaction w
Autor:
Alexey V. Feofanov, A. S. Arseniev, Mikhail P. Kirpichnikov, Oksana V. Nekrasova, M. A. Skutel, A. L. Primak
Publikováno v:
Russian Journal of Bioorganic Chemistry. 46:1011-1017
Recombinant chimeras of hongotoxin 1 (HgTx1) fused through the N - or C-terminus with the red fluorescent protein TagRFP, RFP-HgTx1 and HgTx1-RFP were obtained. The properties of these fluorescent chimeras as ligands of potassium voltage-gated Kv1 ch