Zobrazeno 1 - 10
of 245
pro vyhledávání: '"Oheim Martin"'
Autor:
Salomon Adi, Oheim Martin
Publikováno v:
EPJ Web of Conferences, Vol 309, p 02003 (2024)
We present two distinct types of ‘smart’ surfaces designed for facilitating the quantitative exploration of dynamic processes occurring at sub-wavelength distances from interfaces, using far-field optical techniques. Based on evanescent waves in
Externí odkaz:
https://doaj.org/article/acb7b5ad879044ea8e333c9db8d7ad28
Publikováno v:
EPJ Web of Conferences, Vol 309, p 04023 (2024)
Simultaneous field- and aperture-plane (back-focal plane, BFP) imaging enriches the information content of fluorescence microscopy. In addition to the usual density and concentration maps of sample-plane images, BFP images provide information on the
Externí odkaz:
https://doaj.org/article/c930b4e3c68e4738b44ebd2c14e354dd
Publikováno v:
EPJ Web of Conferences, Vol 309, p 04013 (2024)
The crossed arrangement of excitation and collection optics is the defining feature of selective plane illumination microscopes. It results in an axial sectioning given by the thickness of the light sheet, whereas the lateral resolution depends on th
Externí odkaz:
https://doaj.org/article/73974346f87648658b34d9821f094311
Publikováno v:
EPJ Web of Conferences, Vol 309, p 02007 (2024)
Emission patterns from molecules at interfaces encode many details about their local environment and their axial position, along the microscope’s optical axis. We introduce an advanced approach that synergizes back focal plane (BFP) imaging with in
Externí odkaz:
https://doaj.org/article/92edabd09cc34f68bafa43d4eb149c26
Autor:
Arjmand, Payvand, Thodika, Samlan Chandran, Bivas, Elsa, Li, Haoyang, Oheim, Martin, Yoshida, Hiroyuki, Brasselet, Etienne, Guillon, Marc
Stimulated Emission Depletion (STED) microscopy has emerged as a powerful technique providing visualization of biological structures at the molecular level in living samples. In this technique, the diffraction limit is broken by selectively depleting
Externí odkaz:
http://arxiv.org/abs/2407.16493
Autor:
Deeg Andreas, Trigo Federico, Hazart Doriane, Delhomme Brigitte, Zar Tchyia, Naiser Thomas, Seebacher Christian, Salomon Adi, Ricard Clément, Uhl Rainer, Oheim Martin
Publikováno v:
EPJ Web of Conferences, Vol 287, p 03002 (2023)
Confocal microscopes have been the workhorses of 3-D biological imaging, but they are slow, offer limited depth penetration and collect only ballistic photons. With their inefficient use of excitation photons they expose biological samples to an ofte
Externí odkaz:
https://doaj.org/article/f740a4cba27f4db8aeb372b619f6e75e
Publikováno v:
EPJ Web of Conferences, Vol 287, p 09034 (2023)
Total internal reflection fluorescence (TIRF) has come of age, but a reliable and easy-to-use tool for calibrating evanescent-wave penetration depths is missing. We provide a test-sample for TIRF and other axial super-resolution microscopies for emit
Externí odkaz:
https://doaj.org/article/9aaaa6475bea4ebb819038c3b093ec08
Recent progress has boosted the resolving power of optical microscopies to spatial dimensions well below the diffraction limit. Yet, axial super-resolution and axial single-molecule localisation typically require more complicated implementations than
Externí odkaz:
http://arxiv.org/abs/2311.00590
Publikováno v:
In Optics Communications 15 August 2024 565
Fluorescence detection, either involving propagating or near-field emission, is widely being used in spectroscopy, sensing and microscopy. Total internal reflection fluorescence (TIRF) confines fluorescence excitation by an evanescent (near-) field a
Externí odkaz:
http://arxiv.org/abs/2001.05416