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Determination of anti-HBsAg IgM monoclonal antibodies in cell culture media by perfusion immunoassay
Autor:
Kristen L. Cousineau, Ananth Annapragada, Gary S. Gall, Hongqi Wang, John M. Brackett, Odin D. Cabal, W. Gerard Robey, Bruce Peterson
Publikováno v:
Journal of Immunological Methods. 200:39-46
A rapid, specific, perfusion immunoassay for active anti-HBsAg monoclonal IgM is described. The immunoassay requires less than 3.5 min per sample. The precision was found to be 3.6% at an IgM concentration of 17 μg/ml. A detection limit of 1 μg/ml