A modified BCG with depletion of enzymes associated with peptidoglycan amidation induces enhanced protection against tuberculosis in mice.

Autor: Shaku MT; DST/NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, University of the Witwatersrand, National Health Laboratory Service, Johannesburg, South Africa.; Center for Tuberculosis Research, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, United States., Um PK; Center for Tuberculosis Research, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, United States., Ocius KL; Department of Chemistry, University of Virginia, Charlottesville, United States., Apostolos AJ; Department of Chemistry, University of Virginia, Charlottesville, United States., Pires MM; Department of Chemistry, University of Virginia, Charlottesville, United States., Bishai WR; Center for Tuberculosis Research, Department of Medicine, Johns Hopkins School of Medicine, Baltimore, United States., Kana BD; DST/NRF Centre of Excellence for Biomedical TB Research, Faculty of Health Sciences, University of the Witwatersrand, National Health Laboratory Service, Johannesburg, South Africa.

Publikováno v: ELife [Elife] 2024 Apr 19; Vol. 13. Date of Electronic Publication: 2024 Apr 19.

Identification of a new family of peptidoglycan transpeptidases reveals atypical crosslinking is essential for viability in Clostridioides difficile .

Autor: Bollinger KW; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA., Müh U; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA., Ocius KL; Department of Chemistry, University of Virginia, Charlottesville, VA, USA., Apostolos AJ; Department of Chemistry, University of Virginia, Charlottesville, VA, USA.; Present address: Haleon, 1211 Sherwood Ave, Richmond, VA 23220., Pires MM; Department of Chemistry, University of Virginia, Charlottesville, VA, USA., Helm RF; Department of Biochemistry, Virginia Tech, Blacksburg, VA, USA., Popham DL; Department of Biological Sciences, Virginia Tech, Blacksburg, VA, USA., Weiss DS; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.; Graduate Program in Genetics, University of Iowa, Iowa City, IA USA., Ellermeier CD; Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA.; Graduate Program in Genetics, University of Iowa, Iowa City, IA USA.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Mar 14. Date of Electronic Publication: 2024 Mar 14.

Amidation of glutamate residues in mycobacterial peptidoglycan is essential for cell wall cross-linking.

Autor: Shaku MT; DSI/NRF Centre of Excellence for Biomedical Tuberculosis (TB) Research, Faculty of Health Sciences, University of the Witwatersrand, National Health Laboratory Service, Johannesburg, South Africa., Ocius KL; Department of Chemistry, University of Virginia, Charlottesville, VA, United States., Apostolos AJ; Department of Chemistry, University of Virginia, Charlottesville, VA, United States., Pires MM; Department of Chemistry, University of Virginia, Charlottesville, VA, United States., VanNieuwenhze MS; Department of Chemistry, Indiana University Bloomington, Bloomington, IN, United States., Dhar N; Global Health Institute, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland., Kana BD; DSI/NRF Centre of Excellence for Biomedical Tuberculosis (TB) Research, Faculty of Health Sciences, University of the Witwatersrand, National Health Laboratory Service, Johannesburg, South Africa.

Publikováno v: Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2023 Aug 24; Vol. 13, pp. 1205829. Date of Electronic Publication: 2023 Aug 24 (Print Publication: 2023).

Metabolic Processing of Selenium-Based Bioisosteres of meso -Diaminopimelic Acid in Live Bacteria.

Autor: Apostolos AJ; Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, United States., Ocius KL; Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, United States., Koyasseril-Yehiya TM; Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003-9298, United States., Santamaria C; Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts 01003-9298, United States.; Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003-9298, United States., Silva JRA; Laboratório de Planejamento e Desenvolvimento de Fármacos, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, Pará 66075-110, Brazil., Lameira J; Laboratório de Planejamento e Desenvolvimento de Fármacos, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, Pará 66075-110, Brazil., Alves CN; Laboratório de Planejamento e Desenvolvimento de Fármacos, Instituto de Ciências Exatas e Naturais, Universidade Federal do Pará, Belém, Pará 66075-110, Brazil., Siegrist MS; Molecular and Cellular Biology Graduate Program, University of Massachusetts, Amherst, Massachusetts 01003-9298, United States.; Department of Microbiology, University of Massachusetts, Amherst, Massachusetts 01003-9298, United States., Pires MM; Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, United States.

Publikováno v: Biochemistry [Biochemistry] 2022 Jul 05; Vol. 61 (13), pp. 1404-1414. Date of Electronic Publication: 2022 Jun 10.