A novel hyperactive variant of the Sleeping Beauty transposase facilitates non-viral genome engineering.

Autor: Ochmann MT; Research Center, Division of Hematology, Cell and Gene Therapy, Paul-Ehrlich-Institut, 63225 Langen, Germany., Miskey C; Research Center, Division of Hematology, Cell and Gene Therapy, Paul-Ehrlich-Institut, 63225 Langen, Germany., Botezatu L; Research Center, Division of Hematology, Cell and Gene Therapy, Paul-Ehrlich-Institut, 63225 Langen, Germany., Sandoval-Villegas N; Research Center, Division of Hematology, Cell and Gene Therapy, Paul-Ehrlich-Institut, 63225 Langen, Germany., Diem T; Research Center, Division of Hematology, Cell and Gene Therapy, Paul-Ehrlich-Institut, 63225 Langen, Germany., Ivics Z; Research Center, Division of Hematology, Cell and Gene Therapy, Paul-Ehrlich-Institut, 63225 Langen, Germany.; Institute of Clinical Immunology, University of Leipzig, 04103 Leipzig, Germany.; Fraunhofer Institute for Cell Therapy and Immunology, 04103 Leipzig, Germany.

Publikováno v: Molecular therapy. Nucleic acids [Mol Ther Nucleic Acids] 2024 Nov 04; Vol. 35 (4), pp. 102381. Date of Electronic Publication: 2024 Nov 04 (Print Publication: 2024).

Comprehensive Scanning Mutagenesis of Human Retrotransposon LINE-1 Identifies Motifs Essential for Function.

Autor: Adney EM; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016.; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205., Ochmann MT; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016.; Division of Medical Biotechnology, Paul Ehrlich Institute, Langen 63225, Germany., Sil S; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016., Truong DM; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016., Mita P; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016., Wang X; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016., Kahler DJ; High Throughput Biology Laboratory, NYU Langone Health, New York 10016., Fenyö D; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016., Holt LJ; Institute for Systems Genetics, NYU Langone Health, New York 10016.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016., Boeke JD; Institute for Systems Genetics, NYU Langone Health, New York 10016 Jef.Boeke@nyulangone.org.; Department of Biochemistry and Molecular Pharmacology, NYU Langone Health, New York 10016.; McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Publikováno v: Genetics [Genetics] 2019 Dec; Vol. 213 (4), pp. 1401-1414. Date of Electronic Publication: 2019 Oct 30.