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Protocol for base resolution mapping of ac4C using RedaC:T-seq

Autor: David Sturgill, Daniel Arango, Shalini Oberdoerffer

Publikováno v: STAR Protocols, Vol 3, Iss 4, Pp 101858- (2022)

Summary: N4-acetylcytidine (ac4C) is an mRNA modification catalyzed by the enzyme N-acetyltransferase 10 (NAT10), with position-dependent effects on mRNA translation. This protocol details a procedure to map ac4C at base resolution using NaBH4-induce

Externí odkaz: https://doaj.org/article/aa1dc54276834a5db290ae18e848c889

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Epigenetic control of Topoisomerase 1 activity presents a cancer vulnerability.

Autor: Lee TH; Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287., Qiao CX; Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287.; Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21287., Kuzin V; Department of Cell and Molecular Biology, Karolinska Institutet, 17177 Stockholm, Sweden., Shi Y; Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287.; Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21287., Ramanaranayan V; Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287.; Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21287., Wu T; Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287.; Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21287.; Present address: Department of Cell Biology, University of Pittsburgh, PA 15261., Zhou X; Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287.; Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21287.; Present address: Department of Biochemistry, St Anne's College, Oxford, UK., Corujo D; Program of Myeloid Neoplasms, Program of Applied Epigenetics, Josep Carreras Leukaemia Research Institute (IJC), 08916 Badalona, Barcelona, Spain., Buschbeck M; Program of Myeloid Neoplasms, Program of Applied Epigenetics, Josep Carreras Leukaemia Research Institute (IJC), 08916 Badalona, Barcelona, Spain.; Germans Trias i Pujol Research Institute (IGTP), Badalona, 08916 Barcelona, Spain., Baranello L; Department of Cell and Molecular Biology, Karolinska Institutet, 17177 Stockholm, Sweden., Oberdoerffer P; Department of Radiation Oncology and Molecular Radiation Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21287.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 Oct 25. Date of Electronic Publication: 2024 Oct 25.