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Imaging and proteomics toolkits for studying organelle contact sites

Autor: Rico Gamuyao, Chi-Lun Chang

Publikováno v: Frontiers in Cell and Developmental Biology, Vol 12 (2024)

Organelle contact sites are regions where two heterologous membranes are juxtaposed by molecular tethering complexes. These contact sites are important in inter-organelle communication and cellular functional integration. However, visualizing these m

Externí odkaz: https://doaj.org/article/c70e97d7d79342dd802eb38775f83ddc

Contact-ID, a tool for profiling organelle contact sites, reveals regulatory proteins of mitochondrial-associated membrane formation

Autor: Kwak, Chulhwan, Shin, Sanghee, Park, Jong-Seok, Jung, Minkyo, Nhung, Truong Thi My, Kang, yeong-Gyun, Lee, Chaiheon, Kwon, Tae-Hyuk, Park, Sang Ki, Mun, Ji Young, Kim, Jong-Seo, Rhee, Hyun-Woo

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America, 2020 Jun 01. 117(22), 12109-12120.

Externí odkaz: https://www.jstor.org/stable/26931255

Complementation Assay Using Fusion of Split-GFP and TurboID (CsFiND) Enables Simultaneous Visualization and Proximity Labeling of Organelle Contact Sites in Yeast.

Autor: Fujimoto, Shintaro¹ (AUTHOR), Tashiro, Shinya¹ (AUTHOR), Tamura, Yasushi¹ (AUTHOR) tamura@sci.kj.yamagata-u.ac.jp

Publikováno v: Contact (25152564). 2/6/2023, p1-10. 10p.

The molecular mechanism of on-demand sterol biosynthesis at organelle contact sites.

Autor: Zung N; Department of Molecular Genetics, Weizmann Institute of Science, Israel., Aravindan N; Interfaculty Institute of Biochemistry, University of Tuebingen, Germany., Boshnakovska A; Department of Cellular Biochemistry, University Medical Center Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), University of Göttingen, Germany.; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Translational Neuroinflammation and Automated Microscopy, Germany.; Max Planck Institute for Multidisciplinary Sciences, D-37077, Germany., Valenti R; Department of Molecular Genetics, Weizmann Institute of Science, Israel., Preminger N; Department of Molecular Genetics, Weizmann Institute of Science, Israel., Jonas F; Department of Molecular Genetics, Weizmann Institute of Science, Israel., Yaakov G; Department of Molecular Genetics, Weizmann Institute of Science, Israel., Willoughby MM; School of Chemistry and Biochemistry, Georgia Institute of Technology, USA.; Biochemistry and Molecular Biology Department, University of Nebraska Medical Center, USA., Homberg B; Department of Cellular Biochemistry, University Medical Center Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), University of Göttingen, Germany.; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Translational Neuroinflammation and Automated Microscopy, Germany.; Max Planck Institute for Multidisciplinary Sciences, D-37077, Germany., Keller J; University Medical Center Göttingen, Institute for Neuropathology, 37077, Germany.; Collaborative Research Center 1190 'Compartmental Gates and Contact Sites in Cells', University of Göttingen, Germany., Kupervaser M; The De Botton Protein Profiling institute of the Nancy and Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Israel., Dezorella N; Electron Microscopy Unit, Chemical Research Support, Weizmann Institute of Science, Israel., Dadosh T; Electron Microscopy Unit, Chemical Research Support, Weizmann Institute of Science, Israel., Wolf SG; Electron Microscopy Unit, Chemical Research Support, Weizmann Institute of Science, Israel., Itkin M; Life Sciences Core Facilities, Weizmann Institute of Science, Israel., Malitsky S; Life Sciences Core Facilities, Weizmann Institute of Science, Israel., Brandis A; Life Sciences Core Facilities, Weizmann Institute of Science, Israel., Barkai N; Department of Molecular Genetics, Weizmann Institute of Science, Israel., Fernández-Busnadiego R; University Medical Center Göttingen, Institute for Neuropathology, 37077, Germany.; Collaborative Research Center 1190 'Compartmental Gates and Contact Sites in Cells', University of Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), University of Göttingen, 37077, Germany.; Faculty of Physics, University of Göttingen, 37077, Germany., Reddi AR; School of Chemistry and Biochemistry, Georgia Institute of Technology, USA., Rehling P; Department of Cellular Biochemistry, University Medical Center Göttingen, Germany.; Cluster of Excellence 'Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells' (MBExC), University of Göttingen, Germany.; Fraunhofer Institute for Translational Medicine and Pharmacology ITMP, Translational Neuroinflammation and Automated Microscopy, Germany.; Max Planck Institute for Multidisciplinary Sciences, D-37077, Germany., Rapaport D; Interfaculty Institute of Biochemistry, University of Tuebingen, Germany., Schuldiner M; Department of Molecular Genetics, Weizmann Institute of Science, Israel.

Publikováno v: BioRxiv : the preprint server for biology [bioRxiv] 2024 May 12. Date of Electronic Publication: 2024 May 12.