Zobrazeno 1 - 10
of 119
pro vyhledávání: '"O.L. Gamborg"'
Publikováno v:
Zeitschrift für Pflanzenphysiologie. 95:377-388
Summary A fast growing cell suspension culture of sorghum ( Sorghum bicolor L. Moench ) has been established. Out of 15 genetic strains, cv. GPR-168 responded most favorably to conditions for callus formation. The callus was friable and upon transfer
Publikováno v:
Radiation Botany. 14:343-346
Protoplasts were subjected to increasing doses of ultra-violet irradiation to determine the effect on nucleic acid and protein synthesis, and also on survival and cell colony forming ability. The ability of soy bean protoplasts to form cell colonies
Publikováno v:
Experimental Cell Research. 85:105-110
Cell division in soybean cells (Glycine max (L.) Merr.) grown as 100 ml batch cultures with a defined medium under continuous light has been partially synchronized by daily exchange of 2 5 of the cell suspension for an equal amount of fresh medium. O
Publikováno v:
Zeitschrift für Pflanzenphysiologie. 96:269-275
Summary Procedures have been developed for the isolation, culture and growth of protoplasts of cell cultures derived from sorghum (Sorghum bicolor L. MOENCH cv. GPR-168). Fusion between protoplasts from cell cultures of sorghum and leaf protoplasts o
Publikováno v:
Plant Science Letters. 14:225-229
A cell suspension culture was initiated from green, haploid callus of Marchantia polymorpha L. Protoplasts were isolated from cultured cells by enzymatic degradation of cell walls. The first division of the cells regenerated from protoplasts was obse
Publikováno v:
Plant Science Letters. 16:367-376
A predominantly haploid ( n = 12 ) suspension culture of Datura innoxia Mill. was used to devise methods for the isolation of auxotrophic cells strains. The method employed the filtration of cultures to produce suspensions consisting mainly of single
Publikováno v:
Plant Science Letters. 3:107-111
High yields of viable protoplasts were produced from leaf tissue of Phaseolus vulgaris L. var. Pinto. Consistent production of protoplasts depended on enzymatic liberation of the protoplasts near pH 7.0. Cultured protoplasts regenerated cell walls wi
Publikováno v:
Physiologia Plantarum. 31:217-220
Internodal segments from 6-weeks-old rape plants (Brassica napus L. cv. Zephyr) were induced to differentiate in vitro producing shoots or shoots and roots on synthetic nutrient medium under controlled conditions. Benzyladenine (BA) alone (5 × 10⁻
Publikováno v:
Plant Science Letters. 10:67-74
Callus with leafy shoots were obtained from immature embryos of sorghum (Sorghum bicolor L. Moench) collected 12–18 days after pollination. The embryos were cultured on a defined agar medium containing Murashige-Skoog (MS) mineral salts supplemente
Publikováno v:
Plant Science Letters. 2:107-113
Cassava plants were regenerated from meristems of five cultivars; Colombia No. 800, Llanera, Venezuela No. 255, Ecuador No. 133 and Mexico No. 35. Using benzyladenine (BA), gibberellic acid (GA3) and naphthaleneacetic acid (NAA) at molar concentratio