Zobrazeno 1 - 10
of 150
pro vyhledávání: '"O. Kohl"'
Publikováno v:
Clinical Neurophysiology. 148:e62
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8470fac01806828efedf34bea082bcee
https://doi.org/10.1016/j.clinph.2023.02.136
https://doi.org/10.1016/j.clinph.2023.02.136
Autor:
Thomas O. Kohl, James A. DeCaprio
Publikováno v:
Cold Spring Harbor protocols. 2020(11)
Yeast cells display cell walls that must first be broken before the addition of detergents for lysis. This method describes the use of glass beads in combination with a mechanical bead beater to disrupt cell walls of both Saccharomyces cerevisiae or
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f66b2fe3baf182dd4c8ab197e3b8492
https://pubmed.ncbi.nlm.nih.gov/33139498
https://pubmed.ncbi.nlm.nih.gov/33139498
Autor:
Thomas O. Kohl, James A. DeCaprio
Publikováno v:
Cold Spring Harbor protocols. 2020(11)
This protocol describes the freezing of yeast in liquid nitrogen (LN2) to form small “beans” that can be ground using a simple propeller-blade coffee grinder. The method is ideally suited for lysate preparations from larger yeast cultures ranging
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d8abe571ea9e6b336f36a0dd0ae95d3
https://pubmed.ncbi.nlm.nih.gov/33139499
https://pubmed.ncbi.nlm.nih.gov/33139499
Autor:
James A. DeCaprio, Thomas O. Kohl
Publikováno v:
Cold Spring Harbor protocols. 2019(2)
This protocol describes the cross-linking of antibodies to either Protein A or G agarose beads using disuccinimidyl suberate (DSS), a bifunctional cross-linker capable of directly reacting with two different amines to form stable amide bonds. Protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::47b63999eab306113f2b21acb4b4240d
https://pubmed.ncbi.nlm.nih.gov/30710026
https://pubmed.ncbi.nlm.nih.gov/30710026
Autor:
Thomas O. Kohl, James A. DeCaprio
Publikováno v:
Cold Spring Harbor Protocols. 2021:pdb.err107714
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7cb44ac388a0069d56644787b203c58
https://doi.org/10.1101/pdb.err107714
https://doi.org/10.1101/pdb.err107714
Akademický článek
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Autor:
James, DeCaprio, Thomas O, Kohl
Publikováno v:
Cold Spring Harbor Protocols. 2020:pdb.top098509
Immunoprecipitation, commonly referred to as IP, involves the binding of proteinaceous antigen in solution by an antigen-specific antibody followed by purification of the antigen–antibody complex via attachment to a solid-phase matrix such as Prote
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::769fee0104e6332f9ffdbfb052171de0
https://doi.org/10.1101/pdb.top098509
https://doi.org/10.1101/pdb.top098509
Autor:
James, DeCaprio, Thomas O, Kohl
Publikováno v:
Cold Spring Harbor Protocols. 2020:pdb.prot098665
Chromatin immunoprecipitation, commonly referred to as ChIP, is a powerful technique for the evaluation of in vivo interactions of proteins with specific regions of genomic DNA. Formaldehyde is used in this technique to cross-link proteins to DNA in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::730eb96dac958eb2663be673f172f62c
https://doi.org/10.1101/pdb.prot098665
https://doi.org/10.1101/pdb.prot098665
Autor:
Thomas O. Kohl, James A. DeCaprio
Publikováno v:
Cold Spring Harbor Protocols. 2020:pdb.prot098582
Differential detergent fractionation of cells is a rapid method for extraction of cytoplasmic and nuclear proteins in preparation of an immunoprecipitation. This method can be applied for use of adherent or suspension cells and can significantly redu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a2a06d057fb20a9aa2f88b540e80c1f
https://doi.org/10.1101/pdb.prot098582
https://doi.org/10.1101/pdb.prot098582
Autor:
Thomas O. Kohl, James A. DeCaprio
Publikováno v:
Cold Spring Harbor Protocols. 2020:pdb.prot098616
The only way to solubilize many antigens for immunoprecipitation is by denaturation. This cell lysis protocol is ideally suited for this purpose to release proteins from complex structures or reveal antibody epitopes hidden within native proteins. Sh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::403f0c2870197915a9c116f57dc5107c
https://doi.org/10.1101/pdb.prot098616
https://doi.org/10.1101/pdb.prot098616