Výsledky vyhledávání - "O. A. Zasedateleva"

Structural Destabilization of Intramolecular Duplexes Improves the Results of DNA Hybridization Analysis

Autor: A. A. Stomakhin, O. A. Zasedateleva, Alexander S. Zasedatelev, Sergey Surzhikov, T. V. Nasedkina, A. Yu. Ikonnikova, D. O. Fesenko, V. O. Pozhitnova

Publikováno v: Biophysics. 63:880-887

This study tested a method designed to correctly identify single nucleotide polymorphisms in DNA sequences that are capable of forming a hairpin. Fragments of the angiotensin (AGT) and cytochrome (CYP2C19) genes with rs699 (T>C) and rs4986893 (G>A),

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::27419c5deeda552fb9ccab4679f11437
https://doi.org/10.1134/s000635091806012x

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Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation

Autor: A. V. Lisitsa, Edward N. Timofeev, Sergey A. Lapa, Alexander V. Chudinov, K. S. Romashova, M. A. Spitsyn, V. E. Kuznetsova, V. E. Shershov, O. A. Zasedateleva, T. O. Guseinov, Sergey P. Radko

Publikováno v: Molecular Biology. 52:854-864

A modification of the enzymatic method for the preparation of combinatorial random DNA libraries, which combines amplification in isolated microvolumes with the simultaneous incorporation of modified nucleotides and subsequent separation of DNA stran

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::750f91d808dc0aa933976b0e87e5a42c
https://doi.org/10.1134/s0026893318060110

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PCR incorporation of dUMPs modified with aromatic hydrocarbon substituents of different hydrophilicities: Synthesis of C5-modified dUTPs and PCR studies using Taq, Tth, Vent (exo-) and Deep Vent (exo-) polymerases

Autor: O. A. Zasedateleva, V. E. Kuznetsova, Sergey Surzhikov, Dmitry A. Yurasov, Alexander V. Chudinov, R. A. Miftakhov, V. E. Shershov

Publikováno v: Bioorganic chemistry. 99

Deoxyuridine triphosphate derivatives (dUTPs) modified at the C5 position of the pyrimidine ring with various aromatic hydrocarbon substituents of different hydrophilicities have been synthesized. The aromatic hydrocarbon substituents were attached t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71e7268a4b7431d54f8c56737c2295b5
https://pubmed.ncbi.nlm.nih.gov/32299018

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Generation of Active Carboxyl Groups on the Surface of a Polyethylene Terephthalate Film and Their Quantitation by Digital Fluorescence Microscopy

Autor: Edward N. Timofeev, T. O. Guseinov, Alexander V. Chudinov, V. E. Barsky, M. A. Spitsyn, Yu. P. Lysov, O. A. Zasedateleva, D. D. Mamaev, V. E. Shershov, R. A. Miftakhov, V. E. Kuznetsova, Alexander S. Zasedatelev, Sergey A. Lapa

Publikováno v: Biophysics. 63:512-518

A method to estimate the concentration of chemically accessible carboxylic groups on the surface of a polyethylene terephthalate (PET) film was developed using the fluorochrome cycanine Cy5 and digital fluorescence microscopy. A method was developed

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::8e25609beaec9c39b08b74984630f356
https://doi.org/10.1134/s0006350918040127

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Novel 5-Alkylcarboxamide-2'-Deoxyuridine-5'-Triphosphates for Enzymatic Synthesis of Highly Modified DNA

Autor: T. O. Guseinov, Alexander V. Chudinov, O. A. Zasedateleva, S. P. Radko, R. A. Miftahov, Edward N. Timofeev, A. V. Lisitsa, Sergey Surzhikov, Alexander S. Zasedatelev, Sergey A. Lapa, V. E. Kuznetsova, V. E. Shershov, M. A. Spitsyn, V. A. Vasiliskov

Publikováno v: Russian Journal of Bioorganic Chemistry. 45:221-223

The synthesis of deoxyuridine triphosphate derivatives with alkyl groups of various chain length attached to the pyrimidine base by the trans-alkene linker was performed. The efficiency of the incorporation of modified nucleotides during the synthesi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::70f5e0d19cdde486902c3019e30aabe3
https://doi.org/10.1134/s1068162019030063

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[Preparation of Modified Combinatorial DNA Libraries via Emulsion PCR with Subsequent Strand Separation]

Autor: S A, Lapa, K S, Romashova, M A, Spitsyn, V E, Shershov, V E, Kuznetsova, T O, Guseinov, O A, Zasedateleva, S P, Radko, E N, Timofeev, A V, Lisitsa, A V, Chudinov

Publikováno v: Molekuliarnaia biologiia. 52(6)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e3c085cc8fd2bf007aa5231b35171e54
https://pubmed.ncbi.nlm.nih.gov/30633241

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The effect of chromophore charge on the incorporation efficiency of fluorescence-labeled nucleotides catalyzed by Taq DNA polymerase in matrix synthesis

Autor: Yu. P. Lysov, V. E. Barsky, Alexander S. Zasedatelev, V. E. Kuznetsova, Sergey Surzhikov, Alexander V. Chudinov, V. E. Shershov, V. A. Vasiliskov, O. A. Zasedateleva, T. O. Guseinov, M. A. Spitsyn

Publikováno v: Biophysics. 60:1013-1015

The effect of the chromophore charge on the efficiency of incorporation of fluorescent-labeled nucleotides into DNA during PCR was studied using three dUTP derivatives that contain different fluorescent labels, i.e., electroneutral, positively charge

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::13ca0d8da30343e95acdb3dc3748e020
https://doi.org/10.1134/s0006350915060238

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[The Effect of the Chromophore Charge on the Efficiency of Incorporation of Fluorescently-labeled Nucleotides in Matrix Synthesis by Taq DNA Polymerase]

Autor: V E, Shershov, V E, Kuznetsova, Yu P, Lysov, T O, Guseinov, V E, Barsky, M A, Spitsyn, O A, Zasedateleva, V A, Vasiliskov, S A, Surzhikov, A S, Zasedatelev, A V, Chudinov

Publikováno v: Biofizika. 60(6)

In order to study the effect of an electrical charge of the chromophore, on the efficiency of incorporation of fluorescently-labeled nucleotides into DNA during PCR, three fluorescently-labeled dUPT, one of which with electroneutral and other two wit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f6493366ebbcbd7799c118e347bdfb2a
https://pubmed.ncbi.nlm.nih.gov/26841520

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Sequence specificity of protein-oligonucleotide interactions and protein isolation on sorptive media containing immobilized protein specific oligonucleotides

Autor: A. Yu. Rubina, V. I. Butvilovskaya, M. V. Tsybulskaya, O. A. Zasedateleva, S. B. Popletaeva, Sergey Surzhikov, M. A. Filippova, Alexander S. Zasedatelev, A. A. Stomakhin

Publikováno v: Molecular Biology. 46:596-604

The sequence specificity of protein—oligonucleotide interactions based on the example of binase interaction with oligodeoxyribonucleotides immobilized in biochip gel elements has been studied. Constants of the preferable binding of binase to the se

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::05be7ed7e770e7b26ed14a0ea6b8cecb
https://doi.org/10.1134/s0026893312040097

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dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase

Autor: V. A. Vasiliskov, Sergey Surzhikov, O. A. Zasedateleva, Igor P. Smirnov, Timur O. Guseinov, V. E. Kuznetsova, Alexander V. Chudinov, M. A. Spitsyn, V. E. Shershov, Roman A Yurasov

Publikováno v: Nucleic Acids Research

To develop structural modifications of dNTPs that are compatible with Taq DNA polymerase activity, we synthesized eight dUTP derivatives conjugated with Cy3 or Cy5 dye analogues that differed in charge and charge distribution throughout the fluoropho

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5797e7dab6a34918530222e55dfbd323
http://europepmc.org/articles/PMC6158613

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