Zobrazeno 1 - 10
of 121
pro vyhledávání: '"O W Merten"'
Autor:
O.-W. Merten
Publikováno v:
The Journal of Gene Medicine. 6:S105-S124
Retroviral vectors are still the vectors that are used in the majority of gene therapy trials for treatment of acquired or inherited diseases. In this review, the present state-of-the-art of the production of retroviral vectors and the most important
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a5b37447d9a1f9dd8b6a469a952550bc
https://doi.org/10.1002/jgm.499
https://doi.org/10.1002/jgm.499
Autor:
O.-W. Merten
Publikováno v:
Cytotechnology. 39:91-116
In contrast to contamination by microbes and mycoplasma, which can be relatively easily detected, viral contamination present a serious threat because of the difficulty in detecting some viruses and the lack of effective methods of treating infected
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c8ade753d172ecfe8f432d9aa35b9458
https://doi.org/10.1023/a:1022969101804
https://doi.org/10.1023/a:1022969101804
Publikováno v:
Gene Therapy. 8:737-745
To date, only adherent cell lines have been used for the generation of packaging cells for the production of type C retrovirus vectors. The large-scale production of high titre retrovirus vectors could benefit from the development of packaging cells
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e24ae4206e6888e6a1ed8833b7c9b713
https://doi.org/10.1038/sj.gt.3301457
https://doi.org/10.1038/sj.gt.3301457
Autor:
O. Danos, C. Bouquet, O.-W. Merten, Pedro E. Cruz, D. Gonçalves, C. Rochette, Manuel J.T. Carrondo, C. Geny-Fiamma
Publikováno v:
Biotechnology Progress. 17:326-335
Improved, human-based packaging cell lines allow the production of high-titer, RCR-free retroviral vectors. The utility of these cell lines for the production of clinical grade vectors critically depends on the definition of optimal conditions for sc
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e7da8efd15ba07d7285e110574ea9841
https://doi.org/10.1021/bp000162z
https://doi.org/10.1021/bp000162z
Autor:
O.-W. Merten
Publikováno v:
Cytotechnology. 34:175-179
The use of the ultrasonic separation deviceis a very important step in the direction forimproving animal cell bioreactor cultures. However,the normal construction of the ultrasonic separationdevice ADI 1015 has an inherent disadvantage inpumping the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::37bca7367237b0661545bac64e030628
https://doi.org/10.1023/a:1008147822625
https://doi.org/10.1023/a:1008147822625
Publikováno v:
Biotechnology and Bioengineering. 44:753-764
During long-term continuous culture of the hybridoma cell line 11317, a better-producing subclone (I1317-SF11), giving improved productivity, has been selected. The comparison of the original cell line (I1317-DC) with this subclone revealed that alth
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::771242d4cf6a174450cd7f36a56a0679
https://doi.org/10.1002/bit.260440612
https://doi.org/10.1002/bit.260440612
Publikováno v:
Cytotechnology. 14:47-59
The presence of serum in cell culture raises safety problems for the production of biologicals, thus a new serum-free medium (MDSS2) was developed. The evaluation of this medium for the growth of different cell lines (BHK-21 C13, BSR and Vero) has sh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::07420a9e180aba0ffb5c2f4695f3882e
https://doi.org/10.1007/bf00772195
https://doi.org/10.1007/bf00772195
Autor:
O W Merten, Sofia Benkhelifa-Ziyyat, Anne Galy, Sabine Charrier, M Ferrand, J Perea, M Zerbato, A. Viornery, G Précigout, Bucher-Laurent S
Publikováno v:
Gene Ther
Gene Ther, 2011, 18 (5), pp.479-87. ⟨10.1038/gt.2010.163⟩
Gene Therapy
Gene Therapy, 2011, 18 (5), pp.479-487. ⟨10.1038/gt.2010.163⟩
Gene Ther, 2011, 18 (5), pp.479-87. ⟨10.1038/gt.2010.163⟩
Gene Therapy
Gene Therapy, 2011, 18 (5), pp.479-487. ⟨10.1038/gt.2010.163⟩
International audience; Lentiviral vectors are effective tools for gene transfer and integrate variable numbers of proviral DNA copies in variable proportions of cells. The levels of transduction of a cellular population may therefore depend upon exp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::617d4ef188539153bca553f1f68bd045
https://hal.archives-ouvertes.fr/hal-02881185
https://hal.archives-ouvertes.fr/hal-02881185
Publikováno v:
Biotechnology and Bioengineering. 39:596-606
Hybridoma I.13.17 was grown in semicontinuous culture in an attempt to investigate the steady-state concentrations of key components of monoclonal antibody (MAb) synthesis (e.g., intracellular MAb, IgG messenger RNAs) at different dilution rates betw
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b79fc076e47918bcb018a50b74a4127a
https://doi.org/10.1002/bit.260390603
https://doi.org/10.1002/bit.260390603
Autor:
O.-W. Merten
Publikováno v:
Cytotechnology. 34:181-183
of the agent provoking the new form of Creutzfeldt Jakob Syndrom) shows again the urgent need for the use of safer (with respect to biological safety) production methods for vaccines (and biologicals, in general) which are applied to the whole popula
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::6eb25b6c42989eb5f6cea5db40cfa4cd
https://doi.org/10.1023/a:1008160817560
https://doi.org/10.1023/a:1008160817560