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pro vyhledávání: '"O S, Frankfurt"'
Autor:
O S, Frankfurt
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 113
Publikováno v:
Clinical cancer research : an official journal of the American Association for Cancer Research. 3(3)
Precise quantitation of apoptotic cells in solid tumors is necessary to determine the role of apoptosis in cancer growth, prognosis, and treatment. In this study, the intensity of apoptotic death was determined in 91 breast carcinomas with a novel ce
Publikováno v:
Anticancer research. 16(4A)
We report application of a novel immunohistochemical procedure for the staining of apoptotic (AP) cells in paraffin sections using monoclonal antibody (MAb) to single-stranded DNA. MAb differentiated between apoptosis and necrosis and in contrast to
Autor:
O S, Frankfurt
Publikováno v:
Anticancer research. 14(5A)
In cultures of leukemic HL-60 and MOLT-4 cells treated with etoposide all nuclei with distinctive morphology of apoptosis (chromatin condensation at the nuclear periphery, nuclear fragmentation) were stained with monoclonal antibody F7-26 specific fo
Publikováno v:
Anticancer research. 13(4)
Aphidicolin (AP) or hydroxyurea (HU) inhibited DNA repair and enhanced cytotoxicity in human ovarian carcinoma cells A2780 treated with L-phenylalanine mustard (L-PAM) combined with cisplatin or thioTEPA, and in the cells treated with cisplatin combi
Publikováno v:
Oncology research. 5(1)
Chronic lymphocytic leukemia and lymphoma cells were treated with antitumor drugs in vitro and analyzed by flow cytometry to measure the number of apoptotic (AP) cells and DNA damage in the cells that escaped apoptotic death. AP cells were identified
Publikováno v:
Cancer research. 51(4)
The effect of L-phenylalanine mustard (L-PAM) on heterogeneous cell populations containing sensitive and resistant cells was evaluated by flow cytometric analysis of DNA damage. Cell cultures were treated with L-PAM for 1 h, fixed, and stained with a
Publikováno v:
Cancer research. 50(15)
DNA damage in the cells sensitive and resistant to alkylating agents was determined by flow cytometry analysis of cells stained with anti-DNA monoclonal antibody (MOAB) F7-26. MOAB F7-26 interacted with single-stranded regions in alkylated DNA, and t
Autor:
O S, Frankfurt
Publikováno v:
Methods in cell biology. 33
Autor:
S. A. Goncharova, O. S. Frankfurt
Publikováno v:
Cell Proliferation. 9:333-340
The influence of methotrexate (MTX) on the proliferative activity of cells in different phases of cell cycle has been studied. MTX (5 mg/kg) was injected i.p. 3 days after the inoculation of 5 × 106 leukemia cells into F1 (DBA × C57 BL) mice. It wa