Heterologous Expression of Thermogutta terrifontis Endo-Xanthanase in Penicillium verruculosum, Isolation and Primary Characterization of the Enzyme

Autor: Alexandra M. Rozhkova, Ilya V. Kublanov, O. G. Korotkova, Ivan N. Zorov, M. V. Semenova, Arkady P. Sinitsyn, Alexandr G. Elcheninov, Yury A. Denisenko

Publikováno v: Biochemistry (Moscow). 86:489-495

Heterologous endo-xanthanase (EX) from the thermophilic planktomycete Thermogutta terrifontis strain was obtained using Penicillium verruculosum 537 (ΔniaD) expression system with the cellobiohydrolase 1 gene promoter. Homogeneous EX with a molecula

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::68b7ca3f6202a8f640b97636975b6739
https://doi.org/10.1134/s000629792104009x

Comparative Analysis of the Properties of Recombinant Endo-1,4-β-Glucanase II and Its Chimeric Form with a Cellular Binding Module

Autor: E. G. Kondrat’eva, M. V. Semenova, O. G. Korotkova, Alexandra M. Rozhkova, Arkady P. Sinitsyn, E. A. Rubtsova, N. M. Bibikov, O. A. Sinitsyna

Publikováno v: Moscow University Chemistry Bulletin. 74:223-228

A chimeric form of Penicillium verruculosum endo-1,4-β-glucanase II (EGII), the C-terminus of which contains a cellulose-binding module (CBM) of P. verruculosum cellobiohydrolase I, is produced by the genetic engineering method. In the native form,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::29c896823ccbe7116f2f650d97bd1a22
https://doi.org/10.3103/s0027131419050067

Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails

Autor: Francisca Contreras, Ivan N. Zorov, Mehdi D. Davari, O. G. Korotkova, Aleksandra M. Rozhkova, Ulrich Schwaneberg, Arkady P. Sinitsyn, Subrata Pramanik

Publikováno v: International Journal of Molecular Sciences, Vol 21, Iss 5, p 1589 (2020)
International Journal of Molecular Sciences
International journal of molecular sciences 21(5), 1589 (2020). doi:10.3390/ijms21051589 special issue: "Special Issue "A Commemorative Issue to Celebrate the Nobel Success of Prof. Frances H. Arnold: Directed Evolution" / Special Issue Editors: Tuck Seng Wong, Guest Editor; Miguel Alcalde, Guest Editor"

International journal of molecular sciences 21(5), 1589 (2020). doi:10.3390/ijms21051589
Published by Molecular Diversity Preservation International, Basel

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::87fa0896d36c01ec98c60b3276bb4058

A New Enzyme Preparation for Reducing the Viscosity of Whole-Grain Rye Extracts

Autor: O. G. Korotkova, Alexandra M. Rozhkova, D. Miarzlou, Arkady P. Sinitsyn, Ivan N. Zorov, I. A. Shashkov, A. V. Bashirova

Publikováno v: Moscow University Chemistry Bulletin. 73:53-57

New recombinant strains based on the lower fungus Penicillium canescens are engineered to produce an extracellular enzyme complex that contains a homologous endo-1,4-β-xylanase E (XylE, EC 3.2.1.8). Expression of the gene encoding XylE is controlled

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::becfb694426db8e5ad92cdb035b0fef3
https://doi.org/10.3103/s0027131418020141

Enhancement of thermostability of GH10 xylanase E Penicillium canescens directed by ΔΔG calculations and structure analysis

Autor: Anna S. Dotsenko, Yury A. Denisenko, Arkady P. Sinitsyn, O. G. Korotkova, Aleksandra M. Rozhkova, Ivan N. Zorov

Publikováno v: Enzyme and Microbial Technology. 152:109938

Hydrolytic enzymes are highly demanded in the industry. Thermostability is an important property of enzymes that affects the economic costs of the industrial processes. The rational design of GH10 xylanase E (XylE) Penicillium canescens for the therm

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0b368b255e25ba158736538516bb0b5
https://doi.org/10.1016/j.enzmictec.2021.109938