Design of Affinity Chromatography Peptide Ligands Through Combinatorial Peptide Library Screening

Autor: G R, Barredo, S L, Saavedra, M C, Martínez-Ceron, S L, Giudicessi, M M, Marani, F, Albericio, O, Cascone, S A, Camperi

Publikováno v: Methods in molecular biology (Clifton, N.J.). 2178

In this chapter, a protocol to design affinity chromatography matrices with short peptide ligands immobilized for protein purification is described. The first step consists of the synthesis of a combinatorial peptide library on the hydroxymethylbenzo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e29374d610fb68a8726888da736b904b
https://pubmed.ncbi.nlm.nih.gov/33128753

Two-step enzymatic strategy for the synthesis of a smart phenolic polymer and further immobilization of a β-galactosidase able to catalyze transglycosydation reaction

Autor: A. Glodowsky, G. Levin, S. Gómez, O. Cascone, M.J. Hernáiz

Publikováno v: International journal of biological macromolecules. 117

A rapid and efficient enzymatic procedure for the preparation of an immobilized β-galactosidase has been described. In a first step, soybean peroxidase was used to catalyze the polymerization of a strategically activated phenol (N–Succinimidyl 3�

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::eae8430870f3ecad0b5d11ed46996bb4
https://pubmed.ncbi.nlm.nih.gov/29807083

Construction of a mutant library of horseradish peroxidase gene by directed evolution and development of an in situ screening method

Autor: Héctor M. Targovnik, Fernando Mendive, O. Cascone, M.V. Miranda, M.M. Segura

Publikováno v: Brazilian Journal of Chemical Engineering, Volume: 20, Issue: 1, Pages: 33-38, Published: MAR 2003
Brazilian Journal of Chemical Engineering, Vol 20, Iss 1, Pp 33-38 (2003)
Brazilian Journal of Chemical Engineering v.20 n.1 2003
Brazilian Journal of Chemical Engineering
Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ

A process of directed evolution applied to obtain a library of mutants of horseradish peroxidase (HRP) enzyme is described. We have introduced slight variations into the original DNA shuffling protocol. A DNA template was prepared by PCR amplificatio

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::587b549b752ebe080e66c8c5884cfd2f
https://doi.org/10.1590/s0104-66322003000100007

Structural glance into a novel anti-staphylococcal peptide

Autor: N B, Iannucci, L M, Curto, F, Albericio, O, Cascone, J M, Delfino

Publikováno v: Biopolymers. 102(1)

Novel antimicrobial peptides are valuable molecules for developing anti-infective drugs to counteract the contemporary spread of microbial drug-resistance. Here we focus on a novel peptide (RKWVWWRNR-NH2) derived from the fragment 107-115 of the huma

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::bace49271c5bbdbcc2ac11138606ba0a
https://pubmed.ncbi.nlm.nih.gov/23982895

Peptide synthesis catalysed by a haloalkaliphilic serine protease from the archaeon Natrialba magadii (Nep)

Autor: D M, Ruiz, N B, Iannuci, O, Cascone, R E, De Castro

Publikováno v: Letters in applied microbiology. 51(6)

Haloarchaeal proteases function optimally in high salt (low water activity); thus, they offer an advantage over the nonhalophilic counterparts as biocatalysts for protease-catalysed peptide synthesis. The haloalkaliphilic archaeon Natrialba magadii s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::1897250a682fd90372054dc5e5e2e3ce
https://pubmed.ncbi.nlm.nih.gov/21039670