Zobrazeno 1 - 10
of 192
pro vyhledávání: '"Nynke H. Dekker"'
Autor:
Humberto Sánchez, Zhaowei Liu, Edo van Veen, Theo van Laar, John F. X. Diffley, Nynke H. Dekker
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (O
Externí odkaz:
https://doaj.org/article/e9cfc90ea0f94fb5a860b8c50a778420
Autor:
Daniel Ramírez Montero, Humberto Sánchez, Edo van Veen, Theo van Laar, Belén Solano, John F. X. Diffley, Nynke H. Dekker
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the way at the front of replication forks. Understanding the motion of CMG on the DNA is therefore key to our understanding of DNA replication. In vivo, C
Externí odkaz:
https://doaj.org/article/ccb1945bc66942e4ba899cf6a738ad2d
Publikováno v:
PLoS ONE, Vol 18, Iss 11 (2023)
Externí odkaz:
https://doaj.org/article/7905c94bab774f578fefafb87cc9534e
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101606- (2022)
Summary: Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp). Here, we present a protocol for simultaneously probing the RNA synthesis dynamics of
Externí odkaz:
https://doaj.org/article/215853359616435f8cd0b037ab4fc12f
Autor:
Humberto Sánchez, Kaley McCluskey, Theo van Laar, Edo van Veen, Filip M. Asscher, Belén Solano, John F. X. Diffley, Nynke H. Dekker
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Eukaryotic DNA replication is regulated to ensure copying of the genome (only) once per cell cycle. Here the authors, using optical trapping and confocal microscopy, demonstrate the dynamics of the origin recognition complex and subsequent intermedia
Externí odkaz:
https://doaj.org/article/48341162ccdd441d85b494952e9dc0d4
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-12 (2020)
Torsional stress is generated during DNA replication and transcription, however, the propagation of twist in condensed chromatin is poorly understood. Here the authors measure how force and torque impact chromatin fibers and find that the fibers fold
Externí odkaz:
https://doaj.org/article/30a70ffb95644882808b1311d671a36e
Autor:
David Dulin, Jamie J. Arnold, Theo van Laar, Hyung-Suk Oh, Cheri Lee, Angela L. Perkins, Daniel A. Harki, Martin Depken, Craig E. Cameron, Nynke H. Dekker
Publikováno v:
Cell Reports, Vol 21, Iss 4, Pp 1063-1076 (2017)
Summary: RNA viruses pose a threat to public health that is exacerbated by the dearth of antiviral therapeutics. The RNA-dependent RNA polymerase (RdRp) holds promise as a broad-spectrum, therapeutic target because of the conserved nature of the nucl
Externí odkaz:
https://doaj.org/article/4bf1a74ecce14305982ecd8c80a25e31
Autor:
David Dulin, Igor D. Vilfan, Bojk A. Berghuis, Susanne Hage, Dennis H. Bamford, Minna M. Poranen, Martin Depken, Nynke H. Dekker
Publikováno v:
Cell Reports, Vol 10, Iss 6, Pp 983-992 (2015)
RNA viruses have specific mutation rates that balance the conflicting needs of an evolutionary response to host antiviral defenses and avoidance of the error catastrophe. While most mutations are known to originate in replication errors, difficulties
Externí odkaz:
https://doaj.org/article/a6b664ecbfa3478b90d40c81b2055d22
Publikováno v:
Cell Reports, Vol 10, Iss 2, Pp 216-225 (2015)
DNA wrapping around histone octamers generates nucleosomes, the basic compaction unit of eukaryotic chromatin. Nucleosome stability is carefully tuned to maintain DNA accessibility in transcription, replication, and repair. Using freely orbiting magn
Externí odkaz:
https://doaj.org/article/44239f5f47f34723a336e2c4b4480b21
Autor:
Humberto Sánchez, Zhaowei Liu, Edo van Veen, Theo van Laar, Belén Solano, John F. X. Diffley, Nynke H. Dekker
Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (ORC). In y
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8ea3fbd58239f0d2f6004221b0c1f434
https://doi.org/10.1101/2023.05.18.541303
https://doi.org/10.1101/2023.05.18.541303
