Zobrazeno 1 - 10
of 192
pro vyhledávání: '"Nynke H Dekker"'
Autor:
Rifka Vlijm, Mina Lee, Orkide Ordu, Anastasiya Boltengagen, Alexandra Lusser, Nynke H Dekker, Cees Dekker
Publikováno v:
PLoS ONE, Vol 10, Iss 10, p e0141267 (2015)
Eukaryotic nucleosomes consists of an (H3-H4)2 tetramer and two H2A-H2B dimers, around which 147 bp of DNA are wrapped in 1.7 left-handed helical turns. During chromatin assembly, the (H3-H4)2 tetramer binds first, forming a tetrasome that likely con
Externí odkaz:
https://doaj.org/article/6a68b8bfe3fd44f2a984b38ac495987f
Autor:
Humberto Sánchez, Zhaowei Liu, Edo van Veen, Theo van Laar, John F. X. Diffley, Nynke H. Dekker
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (O
Externí odkaz:
https://doaj.org/article/e9cfc90ea0f94fb5a860b8c50a778420
Autor:
Daniel Ramírez Montero, Humberto Sánchez, Edo van Veen, Theo van Laar, Belén Solano, John F. X. Diffley, Nynke H. Dekker
Publikováno v:
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023)
Abstract The eukaryotic replicative helicase CMG centrally orchestrates the replisome and leads the way at the front of replication forks. Understanding the motion of CMG on the DNA is therefore key to our understanding of DNA replication. In vivo, C
Externí odkaz:
https://doaj.org/article/ccb1945bc66942e4ba899cf6a738ad2d
Publikováno v:
PLoS ONE, Vol 18, Iss 11 (2023)
Externí odkaz:
https://doaj.org/article/7905c94bab774f578fefafb87cc9534e
Publikováno v:
STAR Protocols, Vol 3, Iss 3, Pp 101606- (2022)
Summary: Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp). Here, we present a protocol for simultaneously probing the RNA synthesis dynamics of
Externí odkaz:
https://doaj.org/article/215853359616435f8cd0b037ab4fc12f
Autor:
Humberto Sánchez, Zhaowei Liu, Edo van Veen, Theo van Laar, Belén Solano, John F. X. Diffley, Nynke H. Dekker
Chromatin replication involves the assembly and activity of the replisome within the nucleosomal landscape. At the core of the replisome is the Mcm2-7 complex (MCM), which is loaded onto DNA after binding to the Origin Recognition Complex (ORC). In y
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::8ea3fbd58239f0d2f6004221b0c1f434
https://doi.org/10.1101/2023.05.18.541303
https://doi.org/10.1101/2023.05.18.541303
Autor:
Kaley McCluskey, Nynke H. Dekker
Publikováno v:
Optics Communications, 538
TIRF (Total Internal Reflection Fluorescence) microscopy is a powerful tool for measuring the intra- and intermolecular dynamics of fluorescently-labeled single molecules. As TIRF measurements move to more complex biological systems with more fluores
Autor:
Daniel Fan, Jelmer Cnossen, Shih-Te Hung, Dimitri Kromm, Nynke H. Dekker, Gerard J. Verbiest, Carlas S. Smith
Publikováno v:
Optics Communications. 542
To better understand the interactions between biological molecules, a high optical resolution in all three dimensions is crucial. The intrinsically lower axial resolution of microscopes however, is a limiting factor in fluorescence imaging, correspon
Autor:
Clément Rouillon, Bruna V Eckhardt, Leonie Kollenstart, Fabian Gruss, Alexander E E Verkennis, Inge Rondeel, Peter H L Krijger, Giulia Ricci, Alva Biran, Theo van Laar, Charlotte M Delvaux de Fenffe, Georgiana Luppens, Pascal Albanese, Koichi Sato, Richard A Scheltema, Wouter de Laat, Puck Knipscheer, Nynke H Dekker, Anja Groth, Francesca Mattiroli
Publikováno v:
Nucleic acids research, 51(8)
During every cell cycle, both the genome and the associated chromatin must be accurately replicated. Chromatin Assembly Factor-1 (CAF-1) is a key regulator of chromatin replication, but how CAF-1 functions in relation to the DNA replication machinery
Publikováno v:
Cell Reports, Vol 10, Iss 2, Pp 216-225 (2015)
DNA wrapping around histone octamers generates nucleosomes, the basic compaction unit of eukaryotic chromatin. Nucleosome stability is carefully tuned to maintain DNA accessibility in transcription, replication, and repair. Using freely orbiting magn
Externí odkaz:
https://doaj.org/article/44239f5f47f34723a336e2c4b4480b21