Expression, purification, and characterization of human mannose-6-phosphate receptor - Extra cellular domain from a stable cell line utilizing a small molecule biomimetic of the mannose-6-phosphate moiety.

Autor: Dwyer B; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA. Electronic address: brian.dwyer@Takeda.com., Lundberg D; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA., Iskenderian A; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA., Strack-Logue B; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA., Pescatore B; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA., Norton AW; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA., Xu J; University of Massachusetts, Lowell, MA, 01854, USA., Meiyappan M; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA., Concino MF; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA., Zhang B; Takeda Pharmaceuticals, Cambridge, MA, 02140, USA.

Publikováno v: Protein expression and purification [Protein Expr Purif] 2020 Jun; Vol. 170, pp. 105589. Date of Electronic Publication: 2020 Feb 03.

Protein Engineering on Human Recombinant Follistatin: Enhancing Pharmacokinetic Characteristics for Therapeutic Application.

Autor: Shen C; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Iskenderian A; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Lundberg D; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., He T; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Palmieri K; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Crooker R; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Deng Q; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Traylor M; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Gu S; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Rong H; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Ehmann D; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Pescatore B; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Strack-Logue B; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Romashko A; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Baviello G; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Gill J; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Zhang B; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Meiyappan M; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Pan C; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.)., Norton AW; Departments of Discovery Therapeutics (C.S., A.I., D.L., B.P., B.S.-L., A.R., G.B., J.G., B.Z., M.M., C.P., A.W.N.), Bioanalytical and Biomarker Development (T.H.), Discovery Biology and Translational Research (K.P., R.C., Q.D., D.E.), Analytical Development (M.T., S.G.), and Drug Metabolism and Pharmacokinetics (H.R.), Shire, Lexington, Massachusetts (H.R.) anorton@shire.com.

Publikováno v: The Journal of pharmacology and experimental therapeutics [J Pharmacol Exp Ther] 2018 Aug; Vol. 366 (2), pp. 291-302. Date of Electronic Publication: 2018 May 11.

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Recombinant human complement component C2 produced in a human cell line restores the classical complement pathway activity in-vitro: an alternative treatment for C2 deficiency diseases.

Autor: Martini PG; Department of Protein Expression and Purification Research, Shire Human Genetic Therapies Inc, 700 Main Street, Cambridge, MA 02139, USA. pmartini@shire.com, Cook LC, Alderucci S, Norton AW, Lundberg DM, Fish SM, Langsetmo K, Jönsson G, Lood C, Gullstrand B, Zaleski KJ, Savioli N, Lottherand J, Bedard C, Gill J, Concino MF, Heartlein MW, Truedsson L, Powell JL, Tzianabos AO

Publikováno v: BMC immunology [BMC Immunol] 2010 Aug 20; Vol. 11, pp. 43. Date of Electronic Publication: 2010 Aug 20.

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