Zobrazeno 1 - 10
of 98
pro vyhledávání: '"Norman H. Giles"'
Publikováno v:
Journal of Heredity. 82:1-7
In Neurospora crassa, five structural genes and two regulatory genes control the use of quinic acid as a carbon source. All seven genes are tightly linked to form the qa gene cluster. The entire cluster, which has been cloned and sequenced, occupies
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::741cb6bf984502d6c3d70a7849c689cd
https://doi.org/10.1093/jhered/82.1.1
https://doi.org/10.1093/jhered/82.1.1
Publikováno v:
Cell. 36:493-502
The function of the qa-1F positive regulatory gene of Neurospora has been studied by mapping the initiation sites for transcription of the clustered qa structural genes in wild type, in qa-1F mutants, and in cis-acting activator protein-independent m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f89fe2e10c588cd772ef475d04e8419
https://doi.org/10.1016/0092-8674(84)90242-3
https://doi.org/10.1016/0092-8674(84)90242-3
Autor:
Norman H. Giles, Brett M. Tyler
Publikováno v:
Proceedings of the National Academy of Sciences. 82:5450-5454
We have developed soluble extracts from Neurospora crassa capable of accurately initiating the transcription of cloned Neurospora protein-encoding genes by RNA polymerase II in vitro. The genes encoding glutamate dehydrogenase (am) and histones H3 an
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3619ebc413fe8cb1ccf210a9d90d89fe
https://doi.org/10.1073/pnas.82.16.5450
https://doi.org/10.1073/pnas.82.16.5450
Publikováno v:
Gene. 4:241-259
Two hybrid plasmids which carry the gene for Neurospora crassa catabolic dehydroquinase (C-DHQase) and complement an aroD6 (dehydroquinase-deficient) auxotroph of Escherichia coli have been analyzed. One of these contains a 2.9 kilobase (kb) fragment
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4b29e64ded4cb0638fb2f224d25a3776
https://doi.org/10.1016/0378-1119(78)90021-5
https://doi.org/10.1016/0378-1119(78)90021-5
Publikováno v:
Gene. 14:73-80
A 5.6-kb PstI fragment containing the structural gene (aroD) for 5-dehydroquinate hydrolyase (DHQase) of Escherichia coli K-12 has been cloned into recombinant plasmid pJKK12. The bacterial fragment contains two BglII, one HpaII, one SalI and one Xho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::30f639ad5730f633337db2f22e7fc2e2
https://doi.org/10.1016/0378-1119(81)90149-9
https://doi.org/10.1016/0378-1119(81)90149-9
Publikováno v:
Nucleic Acids Research. 12:5757-5765
A single tRNALeu gene has been localized and sequenced from Neurospora crassa. It is located only 375 bp from the qa gene cluster and it is the only tRNA or 5S rRNA gene within this cloned 37 kb region. The gene encodes a tRNALeu with the anti-codon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e59ac63afb0dbc572245f61692ca8b14
https://doi.org/10.1093/nar/12.14.5757
https://doi.org/10.1093/nar/12.14.5757
Autor:
Norman H. Giles, Mary E. Case
Publikováno v:
Proceedings of the National Academy of Sciences. 72:553-557
The first three reactions in the catabolism of qainic acid in Neurospora crassa are under the genetic control of the qa gene cluster. This cluster consists of three structural genes encoding three inducible enzymes plus a regulatory gene (qa-1+) whos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8236447c0b54607ab4c1ec11b971614c
https://doi.org/10.1073/pnas.72.2.553
https://doi.org/10.1073/pnas.72.2.553
Publikováno v:
Proceedings of the National Academy of Sciences. 76:5259-5263
An efficient transformation system has been developed for Neurospora crassa that uses spheroplasts and pVK88 plasmid DNA. pVK88 is a recombinant Escherichia coli plasmid carrying the N. crassa qa-2 + gene which encodes catabolic dehydroquinase (3-deh
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9d6da9148cbb4ffdddf215b696ca619
https://doi.org/10.1073/pnas.76.10.5259
https://doi.org/10.1073/pnas.76.10.5259
Autor:
Norman H. Giles, Michael Schweizer, Virginia B. Patel, Sidney R. Kushner, Christine C. Dykstra
Publikováno v:
Proceedings of the National Academy of Sciences. 78:5783-5787
A transcription map of the qa gene cluster of Neurospora crassa has been constructed by using cloned DNA fragments as hybridization probes. The mRNAs encoded in the previously identified qa-2 (3-dehydroquinate hydro-lyase, EC 4.2.1.10), qa-4 (dehydro
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::796f7a198f7492e6c931c660f294db33
https://doi.org/10.1073/pnas.78.9.5783
https://doi.org/10.1073/pnas.78.9.5783
Publikováno v:
Gene. 14:23-32
A 22.2-kb insert of Neurospora crassa DNA containing at least two of the genes from the inducible catabolic quinic acid pathway has been cloned into the cosmid vehicle pHC79 resulting in a recombinant plasmid, pMSK-308. The qa-2 + locus (which encode
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e408def3fb1a8d82c72414cac2aed8e
https://doi.org/10.1016/0378-1119(81)90145-1
https://doi.org/10.1016/0378-1119(81)90145-1