A novel N-acetylglutamate synthase architecture revealed by the crystal structure of the bifunctional enzyme from Maricaulis maris.

Autor: Dashuang Shi, Yongdong Li, Juan Cabrera-Luque, Zhongmin Jin, Xiaolin Yu, Gengxiang Zhao, Nantaporn Haskins, Norma M Allewell, Mendel Tuchman

Publikováno v: PLoS ONE, Vol 6, Iss 12, p e28825 (2011)

Novel bifunctional N-acetylglutamate synthase/kinases (NAGS/K) that catalyze the first two steps of arginine biosynthesis and are homologous to vertebrate N-acetylglutamate synthase (NAGS), an essential cofactor-producing enzyme in the urea cycle, we

Externí odkaz: https://doaj.org/article/de8dae987bdc4042b99fb83b43ae9ad3

The N-Acetylglutamate Synthase Family: Structures, Function and Mechanisms

Autor: Norma M. Allewell, Mendel Tuchman, Dashuang Shi

Publikováno v: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 16, Iss 6, Pp 13004-13022 (2015)
Volume 16
Issue 6
Pages 13004-13022

N-acetylglutamate synthase (NAGS) catalyzes the production of N-acetylglutamate (NAG) from acetyl-CoA and l-glutamate. In microorganisms and plants, the enzyme functions in the arginine biosynthetic pathway, while in mammals, its major role is to pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b32da58171a9c4fab28aef410b3b170c
http://europepmc.org/articles/PMC4490483

Structure and function ofEscherichia coliRimK, an ATP-grasp fold,l-glutamyl ligase enzyme

Autor: Mendel Tuchman, Yanli Wang, Norma M. Allewell, Dashuang Shi, Zhongmin Jin, Gengxiang Zhao

Publikováno v: Proteins: Structure, Function, and Bioinformatics. 81:1847-1854

We report herein the crystal structure of Escherichia coli RimK at a resolution of 2.85 A, an enzyme that catalyzes the post-translational addition of up to 15 C-terminal glutamate residues to ribosomal protein S6. The structure belongs to the ATP-gr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::ea4c722348b8afadf75e9c3ae5fcd5fc
https://doi.org/10.1002/prot.24311

Introduction to Biofilms Thematic Minireview Series

Autor: Norma M. Allewell

Publikováno v: The Journal of biological chemistry. 291(24)

The biofilms that many bacteria and fungi produce enable them to form communities, adhere tightly to surfaces, evade host immunity, and resist antibiotics. Pathogenic microorganisms that form biofilms are very difficult to eradicate and thus are a fr

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45f4ee44d1e150d099caac142f79117e
https://pubmed.ncbi.nlm.nih.gov/27129220

The Crystal Structure of N-Acetyl-L-glutamate Synthase from Neisseria gonorrhoeae Provides Insights into Mechanisms of Catalysis and Regulation

Autor: Xiaolin Yu, Hiroki Morizono, Mendel Tuchman, Vatsala Sagar, Dashuang Shi, Norma M. Allewell, Ljubica Caldovic, Zhongmin Jin

Publikováno v: Journal of Biological Chemistry. 283:7176-7184

The crystal structures of N-acetylglutamate synthase (NAGS) in the arginine biosynthetic pathway of Neisseria gonorrhoeae complexed with acetyl-CoA and with CoA plus N-acetylgluta-mate have been determined at 2.5- and 2.6-Å resolution, respectively.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6407fab15d05fc055901153fc8f99deb
https://doi.org/10.1074/jbc.m707678200