Zobrazeno 1 - 10
of 16
pro vyhledávání: '"Noriko Sasakawa"'
Autor:
Peter Gee, Mandy S. Y. Lung, Yuya Okuzaki, Noriko Sasakawa, Takahiro Iguchi, Yukimasa Makita, Hiroyuki Hozumi, Yasutomo Miura, Lucy F. Yang, Mio Iwasaki, Xiou H. Wang, Matthew A. Waller, Nanako Shirai, Yasuko O. Abe, Yoko Fujita, Kei Watanabe, Akihiro Kagita, Kumiko A. Iwabuchi, Masahiko Yasuda, Huaigeng Xu, Takeshi Noda, Jun Komano, Hidetoshi Sakurai, Naoto Inukai, Akitsu Hotta
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Expression of Cas9 and gRNA from viral vectors in vivo may cause off-target activity. Here the authors present NanoMEDIC, which uses nanovesicles to transiently deliver editing machinery to hard-to-transfect cells.
Externí odkaz:
https://doaj.org/article/4d0fe2ef9a604f0fb6865d1e08b1ed50
Autor:
Hiroyuki Morisaka, Kazuto Yoshimi, Yuya Okuzaki, Peter Gee, Yayoi Kunihiro, Ekasit Sonpho, Huaigeng Xu, Noriko Sasakawa, Yuki Naito, Shinichiro Nakada, Takashi Yamamoto, Shigetoshi Sano, Akitsu Hotta, Junji Takeda, Tomoji Mashimo
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Class 1 CRISPR systems are not as developed for genome editing as Class 2 systems are. Here the authors show that Cas3 can be used to generate functional knockouts and knock-ins, as well as Cas3-mediated exon-skipping in DMD cells.
Externí odkaz:
https://doaj.org/article/dce5c9fe636b4d0abf512a35936827ae
Autor:
Hongmei Lisa Li, Naoko Fujimoto, Noriko Sasakawa, Saya Shirai, Tokiko Ohkame, Tetsushi Sakuma, Michihiro Tanaka, Naoki Amano, Akira Watanabe, Hidetoshi Sakurai, Takashi Yamamoto, Shinya Yamanaka, Akitsu Hotta
Publikováno v:
Stem Cell Reports, Vol 4, Iss 1, Pp 143-154 (2015)
Summary: Duchenne muscular dystrophy (DMD) is a severe muscle-degenerative disease caused by a mutation in the dystrophin gene. Genetic correction of patient-derived induced pluripotent stem cells (iPSCs) by TALENs or CRISPR-Cas9 holds promise for DM
Externí odkaz:
https://doaj.org/article/af9517c42803477fb21eb40b3d01039e
Autor:
Hideto Matsui, Naoko Fujimoto, Noriko Sasakawa, Yasuhide Ohinata, Midori Shima, Shinya Yamanaka, Mitsuhiko Sugimoto, Akitsu Hotta
Publikováno v:
PLoS ONE, Vol 9, Iss 8, p e104957 (2014)
Viral vectors have been used for hemophilia A gene therapy. However, due to its large size, full-length Factor VIII (FVIII) cDNA has not been successfully delivered using conventional viral vectors. Moreover, viral vectors may pose safety risks, e.g.
Externí odkaz:
https://doaj.org/article/d34c366589754fad98ba278a41c0b6c9
Autor:
Akitsu Hotta, Miyuki Ono, Peter Gee, Noriko Sasakawa, Takahiro Iguchi, Mandy S. Y. Lung, Xiou H. Wang, Akihiro Kagita, Huaigeng Xu, Yuto Kita
Publikováno v:
Stem Cell Reports
Summary Combined with CRISPR-Cas9 technology and single-stranded oligodeoxynucleotides (ssODNs), specific single-nucleotide alterations can be introduced into a targeted genomic locus in induced pluripotent stem cells (iPSCs); however, ssODN knockin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e604dbc1a61902e6c12c6e8541669855
http://hdl.handle.net/2433/261986
http://hdl.handle.net/2433/261986
Autor:
Kumiko Iwabuchi, Akihiro Kagita, Lucy F. Yang, Nanako Shirai, Takahiro Iguchi, Yoko Fujita, Jun Komano, Takeshi Noda, Akitsu Hotta, Noriko Sasakawa, Yukimasa Makita, Matthew A. Waller, Naoto Inukai, Peter Gee, Mio Iwasaki, Mandy S. Y. Lung, Hidetoshi Sakurai, Yasuko O. Abe, Xiou H. Wang, Yasutomo Miura, Kei Watanabe, Masahiko Yasuda, Hiroyuki Hozumi, Huaigeng Xu, Yuya Okuzaki
Publikováno v:
Nature Communications, Vol 11, Iss 1, Pp 1-18 (2020)
Nature Communications
Nature Communications
Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellul
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6ee31e2e65e6f5c8105713123b385118
http://hdl.handle.net/2433/259354
http://hdl.handle.net/2433/259354
Autor:
Yulia Zhitnyuk, Noriko Sasakawa, Huaigeng Xu, Mandy S. Y. Lung, Hirohide Saito, Akitsu Hotta, Peter Gee
Publikováno v:
Biochemical and Biophysical Research Communications. 505:1097-1102
The delivery of mRNA is advantageous over DNA delivery as it is transient and does not carry the risk of genomic DNA integration. However, there are currently few efficient mRNA delivery options available, especially for hard-to-transfect cell types,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d0a2b1c6555f3abd76426ea10b81e127
https://doi.org/10.1016/j.bbrc.2018.09.113
https://doi.org/10.1016/j.bbrc.2018.09.113
Autor:
Peter Gee, Noriko Sasakawa, Huaigeng Xu, Akitsu Hotta, Julia Alexandra Kudryashev, Kentaro Ishida, Mandy S. Y. Lung
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018)
Scientific Reports
Scientific Reports
Randomized mutagenesis at an endogenous chromosomal locus is a promising approach for protein engineering, functional assessment of regulatory elements, and modeling genetic variations. In mammalian cells, however, it is challenging to perform site-s
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::03e592fb82cf4d8b254b3e044d29f065
http://link.springer.com/article/10.1038/s41598-017-18568-4
http://link.springer.com/article/10.1038/s41598-017-18568-4
Autor:
Shinichiro Nakada, Kazuto Yoshimi, Tomoji Mashimo, Yuki Naito, Yuya Okuzaki, Peter Gee, Takashi Yamamoto, Yayoi Kunihiro, Shigetoshi Sano, Akitsu Hotta, Hiroyuki Morisaka, Huaigeng Xu, Ekasit Sonpho, Junji Takeda, Noriko Sasakawa
Publikováno v:
Nature Communications, Vol 10, Iss 1, Pp 1-13 (2019)
Nature Communications
Nature Communications
Although single-component Class 2 CRISPR systems, such as type II Cas9 or type V Cas12a (Cpf1), are widely used for genome editing in eukaryotic cells, the application of multi-component Class 1 CRISPR has been less developed. Here we demonstrate tha
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9fe0f93a0ea6b763c2c5874885a001a6
http://hdl.handle.net/2433/251038
http://hdl.handle.net/2433/251038
Publikováno v:
The Journal of Immunology. 204:86.6-86.6
Lymphocytes infiltrating to the injured skeletal muscle have been shown to play an important role in muscle regeneration. Site-specific infiltrating/expanding lymphocytes and its molecular features are potential resources for bioengineering such as a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::02e5e4043d4b53ffc23fd4b97a45f2dd
https://doi.org/10.4049/jimmunol.204.supp.86.6
https://doi.org/10.4049/jimmunol.204.supp.86.6