Zobrazeno 1 - 10
of 23
pro vyhledávání: '"Noriko Kusukawa"'
Autor:
Valerie Burland, Noriko Kusukawa
Publikováno v:
BioTechniques, Vol 23, Iss 6, Pp 1070-1075 (1997)
We demonstrate a rapid cloning and sequencing strategy for kilobase-size DNA segments using DNase I and long PCR. In a single-tube protocol, deletions were formed in a plasmid insert by two enzymatic cuts, one at a fixed site and one at random. The d
Externí odkaz:
https://doaj.org/article/55065fa9f01f4b55a42025fcdf745f33
Autor:
Hugh W. White, Noriko Kusukawa
Publikováno v:
BioTechniques, Vol 22, Iss 5, Pp 976-980 (1997)
Short tandem repeats (STRs) are traditionally analyzed on large polyacrylamide electrophoresis gels. We demonstrate in this study that a small (10-cm-long, 1-mm-thick) agarose gel is sufficient for analysis of multiplexed samples for several commonly
Externí odkaz:
https://doaj.org/article/7a203a3630174220883c9ff3bb07a4cc
Autor:
Minjie Wu, Noriko Kusukawa
Publikováno v:
BioTechniques, Vol 24, Iss 4, Pp 676-678 (1998)
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor® XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel stre
Externí odkaz:
https://doaj.org/article/345a17f4b10341b28ab51ca55c2e940c
Publikováno v:
BioTechniques, Vol 21, Iss 1, Pp 142-144 (1996)
Accurate resolution of PCR products in the range of 15–40 kb may be obtained in agarose gels without pulsed-field electrophoresis. A gel of 0.3% SeaKem® Gold agarose cast on GelBond® support film provides good resolution and sufficient gel streng
Externí odkaz:
https://doaj.org/article/1da290c884c54d5fa202c6f5102a9946
Autor:
Noriko Kusukawa, Carl T. Wittwer
Publikováno v:
Molecular Microbiology. :63-82
This chapter talks about the fundamentals of real-time PCR and melting analysis. It draws an analogy between bacterial growth and PCR and then considers the kinetic requirements of PCR. It provides an overview of real-time instrumentation and fluores
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::0988e29e32d9e5bcc7efa78c93644660
https://doi.org/10.1128/9781555816834.ch4
https://doi.org/10.1128/9781555816834.ch4
Publikováno v:
Electrophoresis. 20:1455-1461
The effect of gelation conditions on the structure and resolving properties of agarose-based DNA sequencing gels were studied. Gels that were formed by rapid cooling gave separation patterns with sharp, well-defined bands, and resolved base repeats t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::784cd6e4ae8503d4a91312e0c97927df
https://doi.org/10.1002/(sici)1522-2683(19990601)20:7<1455::aid-elps1455>3.0.co
https://doi.org/10.1002/(sici)1522-2683(19990601)20:7<1455::aid-elps1455>3.0.co
Autor:
Jacquelyn M. O'Connor, Noriko Kusukawa, Valerie Burland, Wendy R. Baubonis, W. Edward Highsmith, Mark M. Garner, Foner P. Curtis, Arun J. Nataraj, Qian Jin
Publikováno v:
Electrophoresis. 20:1186-1194
A systematic characterization of the effects of important physical parameters on the sensitivity and specificity of methods in searching for unknown base changes (mutations or single nucleotide polymorphisms) over a relatively long DNA segment has no
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::547efdacc6e5d5960432aa8509b4e82b
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1186::aid-elps1186>3.0.co
Publikováno v:
Electrophoresis. 20:1177-1185
Single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are popular electrophoretic methods for the identification of sequences. The principle reasons for the popularity of these two methods are their technical simplicity and th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::99b269e179ad157501be2b4719359495
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1177::aid-elps1177>3.0.co
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1177::aid-elps1177>3.0.co
Autor:
W. Edward Highsmith, Jacquelyn M. O'Connor, Mark M. Garner, Sohbi H. El-Nabi, Qian Jin, Noriko Kusukawa, Arun J. Nataraj
Publikováno v:
Electrophoresis. 20:1195-1203
Single-strand conformation polymorphism (SSCP) is one of the most commonly used methods for searching for unknown base changes (mutations). In order to characterize systematically the effects of important physical parameters on the sensitivity and sp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::be198c700a58efab9ed7af2690dda738
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1195::aid-elps1195>3.0.co
https://doi.org/10.1002/(sici)1522-2683(19990101)20:6<1195::aid-elps1195>3.0.co
Autor:
Gareth L. Gardiner, John W. Rose, Thomas B. Martins, Dee Husebye, Harry R. Hill, Noriko Kusukawa
Publikováno v:
Journal of interferoncytokine research : the official journal of the International Society for Interferon and Cytokine Research. 33(2)
Patients with therapy-induced neutralizing antibodies (NAbs) to interferon-beta (IFN-β) have reduced responses to IFN-β treatment, resulting in higher relapse rates, increased magnetic resonance imaging activity, and a higher risk of disease progre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bea2e344ce05c28d01b593dbcd3cae45
https://pubmed.ncbi.nlm.nih.gov/23153300
https://pubmed.ncbi.nlm.nih.gov/23153300