Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Norihiko Oguri"'
Publikováno v:
Equine Veterinary Journal. 25:91-94
Summary Blastocysts (5 unexpanded, 5 expanding, 2 hatched) were recovered non-surgically between 144 and 192 h after detection of ovulation. Unexpanded and expanding embryos were cultured (TCM199 + 10% foetal bovine serum) for 10 days. Mean diameter
Autor:
Norihiko Oguri, Shinichi Hochi, Kiyoshi Miyazawa, Mikiko Kobayashi, Young H. Choi, Kunitada Sato, Missaka P.B. Wijayagunawardane, Ana Yimiko Kojima, Mitsuhiro Takagi
Publikováno v:
Journal of Equine Science. 9:101-106
The present study was conducted to investigate the effects of skim milk glucose (SMG), percoll and Brackett and Oliphant medium supplemented with BSA (BO-BSA) washing and glycosaminoglycans treatments (GAG) on lysophosphatidylcholin (LPC)-induced acr
Publikováno v:
Theriogenology. 46:1217-1224
The present study was designed to examine the suitability of ethylene glycol as a cryoprotectant for equine embryos. Blastocysts recovered nonsurgically from Day 6 donor mares were cryopreserved by conventional 2-step freezing in the presence of 10%
Publikováno v:
Journal of Equine Veterinary Science. 16:244-248
Summary The purpose of this study was to evaluate the ability of various chemicals to induce capacitation of stallion spermatozoa using 2 different assay systems. In Experiment 1, freshly ejaculated spermatozoa were treated for 0, 3 and 6 h with 10
Publikováno v:
Theriogenology. 44:287-294
This study was conducted to clarify the effects of sperm concentration and media during preincubation on fertilization and development of porcine oocytes fertilized in vitro (IVF). The effect of porcine oviduct epithelial cell aggregates (POECA) on i
Autor:
J. Braun, Akio Oka, Kunitada Sato, Fernando Torres-Boggino, Shinichi Hochi, Norihiko Oguri, Yukio Kanno
Publikováno v:
Journal of Veterinary Medical Science. 57:225-229
Seminal characteristics, fertility and the response to semen preservation (liquid storage and cryopreservation) were evaluated in 4 Draft stallions (Percheron 2, Breton 2). Seminal characteristics (gel-free volume, sperm concentration, sperm morpholo
Publikováno v:
Journal of Reproduction and Development. 41:367-371
The ability of equine blastocysts to produce embryonic capsule was investigated in vitro. Blastocysts were nonsurgically recovered from Day-6 donor mares (Day-0; detection of ovulation), and were subjected to 5 days of culture at 37 C in 5% CO2 in ai
Publikováno v:
Journal of Reproduction and Development. 40:13-18
The influence of sera in culture medium on hatching of equine blastocysts was examined. Non-surgically recovered early blastocysts were cultured for 10 days in TCM199 supplemented with 10% (v/v) of: (1) fetal bovine serum (FBS, n=5), (2) fetal equine
Publikováno v:
Journal of Reproduction and Development. 40:243-249
This study was designed to investigate the relationship between embryonic volume changes in response to addition of glycerol and the in vitro survival of frozen-thawed equine blastocysts. Unexpanded blastocysts (diameter 300 μm; n=9) shrunk graduall
Publikováno v:
Journal of Equine Science. 5:77-81
Comparative experiments were conducted to assess the effect o f individual stallions on the fertilizing ability o f the spermatozoa in in-vitro-matured and partially zona-removed (PZR) equine oocytes (Experiment 1), as well as the effect o f mare bre