Zobrazeno 1 - 10
of 33
pro vyhledávání: '"Norbert Klauke"'
Autor:
Norbert Klauke, Huabing Yin, Guangcheng Wang, Lulu Cai, Neng Qiu, Andrew Glidle, Yongkui Zhang, Hang Song, Lijuan Chen, Wenwen Wang, Bozhi Ji, Liang Ma
Publikováno v:
Materials Science and Engineering: C. 32:2634-2640
Biogenic calcium carbonate has come to the attention of many researchers as a promising drug delivery system due to its safety, pH sensitivity and the large volume of information already in existence on its medical use. In this study, we employed bov
Publikováno v:
ELECTROPHORESIS. 31:1405-1413
An array of PDMS microchambers was aligned to an array of sensor electrodes and stimulating microelectrodes, which was used for the electrochemical monitoring of the metabolic activity of single isolated adult ventricular myocytes inside the chamber
Publikováno v:
Analytical Chemistry. 81:6390-6398
The regional manipulation of the microenvironment surrounding single adult cardiac myocytes in a microfluidic structure is described. The flow rates of laminar streams were adjusted such that the fluid interface between an injection flow and a perfus
Autor:
Helmut Plattner, Martin Hardt, Joachim Hentschel, Norbert Klauke, Marie-Pierre Blanchard, Marc R. Husser
Publikováno v:
Cell Calcium. 36:349-358
We asked to what extent Ca(2+) signals in two different domains of Paramecium cells remain separated during different stimulations. Wild-type (7S) and pawn cells (strain d4-500r, without ciliary voltage-dependent Ca(2+)-channels) were stimulated for
Publikováno v:
ResearcherID
Microchannels (40- microm wide, 10- microm high, 10-mm long, 70- microm pitch) were patterned in the silicone elastomer, polydimethylsiloxane on a microscope coverslip base. Integrated within each microchamber were individually addressable stimulatio
Autor:
Norbert Klauke, Godfrey L. Smith, Peter H. Cobbold, Xinxia Cai, Andrew Glidle, Jonathan M. Cooper
Publikováno v:
Analytical Chemistry. 74:908-914
The fabrication of microelectrodes integrated within ultra-low-volume microtiter chambers for the amperometric determination of metabolites continues to be of interest in the subject of single-cell and high-throughput screening. The microsystem descr
Autor:
Norbert Klauke, Helmut Plattner
Publikováno v:
The Journal of Membrane Biology. 176:237-248
The lipophilic fluorescent dye, FM1-43, as now frequently used to stain cell membranes and to monitor exo-endocytosis and membrane recycling, induces a cortical [Ca(2+)](i) transient and exocytosis of dense core vesicles ("trichocysts") in Paramecium
Autor:
Helmut Plattner, Norbert Klauke, Markus Gössringer, Massoud Momayezi, Karin Hauser, Roland Kissmehl
Publikováno v:
Histochemistry and Cell Biology. 110:1-8
We have localized a structure-bound fraction of the exocytosis-sensitive phosphoprotein, PP63/parafusin (PP63/pf), in Paramecium cells by widely different methods. We combined cell fractionation, western blots, as well as light and electron microscop
Publikováno v:
Cell Calcium. 23:349-360
This is a detailed characterization of a secretory mutant incapable of releasing secretory contents despite normal exocytotic membrane fusion performance. Trichocyst non-discharge strain tndl of Paramecium caudatum and its wildtype (wt) both show a t
Autor:
Norbert Klauke, Helmut Plattner
Publikováno v:
Journal of Membrane Biology. 161:65-81
Caffeine causes a [Ca2+]i increase in the cortex of Paramecium cells, followed by spillover with considerable attenuation, into central cell regions. From [Ca2+]resti approximately 50 to 80 nm, [Ca2+]acti rises within/=3 sec to 500 (trichocyst-free s