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Surface display vectors for selective detection and isolation of high level antibody producing cells
Autor:
Hans-Peter Knopf, Thomas Jostock, Sabine Lang, Johannes Wichter, Nommay Audrey Josiane, Delia Drewello, Burkhard Wilms
Publikováno v:
Biotechnology and Bioengineering. 113:2386-2393
Cell line generation for production of biopharmaceuticals in mammalian cells usually involves intensive screening of clones to identify the rare high producers. In order to facilitate efficient and selective fluorescence activated cell sorting (FACS)