Allosteric regulation by cooperative conformational changes of actin filaments drives mutually exclusive binding with cofilin and myosin.

Autor: Ngo KX; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan.; Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo 169-8555, Japan., Umeki N; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan., Kijima ST; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan.; Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan.; Miyakonojo National College of Technology, Miyakonojo, Miyazaki 885-8567, Japan., Kodera N; Department of Physics and Bio-AFM Frontier Research Center, Kanazawa University, Kanazawa, Ishikawa 920-1192, Japan.; PRESTO, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan., Ueno H; Department of Applied Sciences, Muroran Institute of Technology, Muroran, Hokkaido 050-8585, Japan., Furutani-Umezu N; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan., Nakajima J; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan.; Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan., Noguchi TQ; Miyakonojo National College of Technology, Miyakonojo, Miyazaki 885-8567, Japan., Nagasaki A; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan., Tokuraku K; Department of Applied Sciences, Muroran Institute of Technology, Muroran, Hokkaido 050-8585, Japan., Uyeda TQ; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki 305-8562, Japan.; Department of Physics, Faculty of Science and Engineering, Waseda University, Tokyo 169-8555, Japan.; Graduate School of Life and Environmental Sciences, University of Tsukuba, Ibaraki 305-8572, Japan.

Publikováno v: Scientific reports [Sci Rep] 2016 Oct 20; Vol. 6, pp. 35449. Date of Electronic Publication: 2016 Oct 20.

ADF/cofilin is not essential but is critically important for actin activities during phagocytosis in Tetrahymena thermophila.

Autor: Shiozaki N; Structural Biosciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan., Nakano K, Kushida Y, Noguchi TQ, Uyeda TQ, Wloga D, Dave D, Vasudevan KK, Gaertig J, Numata O

Publikováno v: Eukaryotic cell [Eukaryot Cell] 2013 Aug; Vol. 12 (8), pp. 1080-6. Date of Electronic Publication: 2013 May 31.

G146V mutation at the hinge region of actin reveals a myosin class-specific requirement of actin conformations for motility.

Autor: Noguchi TQ; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi, Tsukuba, Ibaraki 305-8562, Japan. t-noguchi@cc.miyakonojo-nct.ac.jp, Komori T, Umeki N, Demizu N, Ito K, Iwane AH, Tokuraku K, Yanagida T, Uyeda TQ

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2012 Jul 13; Vol. 287 (29), pp. 24339-45. Date of Electronic Publication: 2012 May 27.

Screening of novel dominant negative mutant actins using glycine targeted scanning identifies G146V actin that cooperatively inhibits cofilin binding.

Autor: Noguchi TQ; Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan., Toya R, Ueno H, Tokuraku K, Uyeda TQ

Publikováno v: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2010 Jun 11; Vol. 396 (4), pp. 1006-11. Date of Electronic Publication: 2010 May 13.

A novel system for expressing toxic actin mutants in Dictyostelium and purification and characterization of a dominant lethal yeast actin mutant.

Autor: Noguchi TQ; Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology Tsukuba Central 4, 1-1-1 Highashi, Tsukuba, Ibaraki 305-8562, Japan., Kanzaki N, Ueno H, Hirose K, Uyeda TQ

Publikováno v: The Journal of biological chemistry [J Biol Chem] 2007 Sep 21; Vol. 282 (38), pp. 27721-7. Date of Electronic Publication: 2007 Jul 26.