Zobrazeno 1 - 10
of 51
pro vyhledávání: '"Nobuyuki Okahashi"'
Publikováno v:
Metabolic Engineering Communications, Vol 18, Iss , Pp e00239- (2024)
Neutrophils are innate immune cells and the first line of defense for the maintenance of homeostasis. However, our knowledge of the metabolic rewiring associated with their differentiation and immune stimulation is limited. Here, quantitative 13C-met
Externí odkaz:
https://doaj.org/article/cdf96db4211a41458577834c482ff982
Autor:
Kazuki Nishimoto, Nobuyuki Okahashi, Masaharu Maruyama, Yoshihiro Izumi, Kohta Nakatani, Yuki Ito, Junko Iida, Takeshi Bamba, Fumio Matsuda
Publikováno v:
Scientific Reports, Vol 13, Iss 1, Pp 1-10 (2023)
Abstract 4-hydroxytamoxifen (OHT) is an anti-cancer drug that induces apoptosis in breast cancer cells. Although changes in lipid levels and mitochondrial respiration have been observed in OHT-treated cells, the overall mechanisms underlying these me
Externí odkaz:
https://doaj.org/article/a1e968cedfe94a57a8d6d4cda9a2426f
Publikováno v:
Microbial Cell Factories, Vol 22, Iss 1, Pp 1-15 (2023)
Abstract Background “ATP wasting” has been observed in 13C metabolic flux analyses of Saccharomyces cerevisiae, a yeast strain commonly used to produce ethanol. Some strains of S. cerevisiae, such as the sake strain Kyokai 7, consume approximatel
Externí odkaz:
https://doaj.org/article/3f86fb82051040f48e464361b3ac590f
Autor:
Masahiko Sugimura, Taisuke Seike, Nobuyuki Okahashi, Yoshihiro Izumi, Takeshi Bamba, Jun Ishii, Fumio Matsuda
Publikováno v:
International Journal of Molecular Sciences, Vol 24, Iss 22, p 16378 (2023)
Saccharomyces cerevisiae is a promising host for the bioproduction of higher alcohols, such as 2,3-butanediol (2,3-BDO). Metabolically engineered S. cerevisiae strains that produce 2,3-BDO via glycolysis have been constructed. However, the specific 2
Externí odkaz:
https://doaj.org/article/0bace5781b90425084df2e3e5a26227c
Autor:
Fumio Matsuda, Kousuke Maeda, Takeo Taniguchi, Yuya Kondo, Futa Yatabe, Nobuyuki Okahashi, Hiroshi Shimizu
Publikováno v:
Metabolic Engineering Communications, Vol 13, Iss , Pp e00177- (2021)
13C-based metabolic flux analysis (13C-MFA) is an essential tool for estimating intracellular metabolic flux levels in metabolic engineering and biology. In 13C-MFA, a metabolic flux distribution that explains the observed isotope labeling data was c
Externí odkaz:
https://doaj.org/article/7b89c38c628645b7977410b088a13e6e
Autor:
Shota Isogai, Nobuyuki Okahashi, Ririka Asama, Tomomi Nakamura, Tomohisa Hasunuma, Fumio Matsuda, Jun Ishii, Akihiko Kondo
Publikováno v:
Metabolic Engineering Communications, Vol 12, Iss , Pp e00169- (2021)
Reconstitution of prenylflavonoids using the flavonoid biosynthetic pathway and prenyltransferases (PTs) in microbes can be a promising attractive alternative to plant-based production or chemical synthesis. Here, we demonstrate that promiscuous micr
Externí odkaz:
https://doaj.org/article/89ab52b2946141c69be1255b6649bb39
Publikováno v:
STAR Protocols, Vol 2, Iss 2, Pp 100492- (2021)
Summary: We describe a protocol for identifying bacteria-derived lipid metabolites produced in the guts using antibiotic-treated mice, liquid chromatography tandem mass spectrometry-based lipidomics, and feature-based molecular spectrum networking (F
Externí odkaz:
https://doaj.org/article/290c041fa3404b84a4fae6224d048033
Isotope Calculation Gadgets: A Series of Software for Isotope-Tracing Experiments in Garuda Platform
Publikováno v:
Metabolites, Vol 12, Iss 7, p 646 (2022)
Isotope tracing is a powerful technique for elucidating intracellular metabolism. Experiments utilizing this technique involve various processes, such as the correction of natural isotopes. Although some previously developed software are available fo
Externí odkaz:
https://doaj.org/article/61fe4fd469f3486183371d244862e98d
Autor:
Shu Yasuda, Nobuyuki Okahashi, Hiroshi Tsugawa, Yusuke Ogata, Kazutaka Ikeda, Wataru Suda, Hiroyuki Arai, Masahira Hattori, Makoto Arita
Publikováno v:
iScience, Vol 23, Iss 12, Pp 101841- (2020)
Summary: Host-microbiota interactions create a unique metabolic milieu that modulates intestinal environments. Integration of 16S ribosomal RNA (rRNA) sequences and mass spectrometry (MS)-based lipidomics has a great potential to reveal the relations
Externí odkaz:
https://doaj.org/article/69fab6c217dc4271b87603e74769e5af
Autor:
Shin Nishiumi, Yoshihiro Izumi, Akiyoshi Hirayama, Masatomo Takahashi, Motonao Nakao, Kosuke Hata, Daisuke Saigusa, Eiji Hishinuma, Naomi Matsukawa, Suzumi M. Tokuoka, Yoshihiro Kita, Fumie Hamano, Nobuyuki Okahashi, Kazutaka Ikeda, Hiroki Nakanishi, Kosuke Saito, Masami Yokota Hirai, Masaru Yoshida, Yoshiya Oda, Fumio Matsuda, Takeshi Bamba
Publikováno v:
Metabolites, Vol 12, Iss 2, p 135 (2022)
In mass spectrometry-based metabolomics, the differences in the analytical results from different laboratories/machines are an issue to be considered because various types of machines are used in each laboratory. Moreover, the analytical methods are
Externí odkaz:
https://doaj.org/article/8145885c47ea46799181ed43e06d661b