Zobrazeno 1 - 10
of 60
pro vyhledávání: '"Nobutoshi Harata"'
Autor:
Nobutoshi Harata, Michael S. Chimenti, Sara B. Mitchell, Sadahiro Iwabuchi, Sjurson Ae, Thomas B. Bair, Knudtson Kl, Tom Yuen Tm, Kolbe D, Hiroyuki Kawano
In patients with the brain disorder dystonia, body movement is severely affected – with involuntary muscle contractions and abnormal postures, causing extensive deterioration of the patient’s quality of life. The most common inherited form of thi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22833b9eb8ecf5287e1d508c0245a030
https://doi.org/10.1101/825505
https://doi.org/10.1101/825505
Publikováno v:
Journal of visualized experiments : JoVE. (95)
High-resolution analysis of the morphology and function of mammalian neurons often requires the genotyping of individual animals followed by the analysis of primary cultures of neurons. We describe a set of procedures for: labeling newborn mice to be
Publikováno v:
Neuron. 49(2):243-256
SummaryThe kinetics of exo-endocytotic recycling could restrict information transfer at central synapses if neurotransmission were entirely reliant on classical full-collapse fusion. Nonclassical fusion retrieval by kiss-and-run would be kinetically
Publikováno v:
Current Eye Research. 30:789-798
To investigate the dynamics of the intracellular Ca2+ concentration ([Ca2+]i) during retinal ischemia in rats with streptozotocin (STZ)-induced diabetes and the effect of gliclazide, a sulfonylurea with a potent free-radical scavenging activity on is
Autor:
Richard W. Tsien, Gong Chen, Erika S. Piedras-Rentería, Geoffrey B. Smith, Yu-Qing Cao, Nobutoshi Harata
Publikováno v:
Neuron. 43:387-400
Several human channelopathies result from mutations in alpha1A, the pore-forming subunit of P/Q-type Ca2+ channels, conduits of presynaptic Ca2+ entry for evoked neurotransmission. We found that wild-type human alpha1A subunits supported transmission
Publikováno v:
Proceedings of the National Academy of Sciences. 101:1063-1068
At central synapses, quantal size is generally regarded as fluctuating around a fixed mean with little change during short-term synaptic plasticity. We evoked quantal release by brief electric stimulation at single synapses visualized with FM 1–43
Autor:
Nobutoshi Harata, Richard W. Tsien, Richard H. Scheller, Jeremy B. Bergsman, Stephen M. Smith
Publikováno v:
Neuron. 41(2):243-256
Synaptic activity causes reductions in cleft [Ca(2+)] that may impact subsequent synaptic efficacy. Using modified patch-clamp techniques to record from single neocortical nerve terminals, we report that physiologically relevant reductions of extrace
Publikováno v:
Neuropharmacology. 45:797-813
At synapses of the mammalian central nervous system, release of neurotransmitter occurs at rates transiently as high as 100 Hz, putting extreme demands on nerve terminals with only tens of functional vesicles at their disposal. Thus, the presynaptic
Publikováno v:
American Journal of Physiology-Cell Physiology. 282:C366-C373
A link between the circadian rhythm and the function of Cl−-permeable γ-aminobutyric acid (GABA) type A (GABAA) receptors on suprachiasmatic nucleus (SCN) neurons was studied by measuring intracellular activity of Cl−( aCl[Formula: see text]) at
Autor:
Richard W. Tsien, Marina Mozhayeva, Alex Aravanis, Jason L. Pyle, Ege T. Kavalali, Nobutoshi Harata
Publikováno v:
Trends in Neurosciences. 24:637-643
The tiny nerve terminals of central synapses contain far fewer vesicles than preparations commonly used for analysis of neurosecretion. Photoconversion of vesicles rendered fluorescent with the dye FM1-43 directly identified vesicles capable of engag